Food and Fermentation Industries >

2023 , Vol. 49 >Issue 6: 162 - 169

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031971

Solid-state fermentation to Pu-erh big leaf tea with Eurotium cristatum SP-5 to produce pectinase

  • YUE Yanchao ,
  • YANG Yunjuan ,
  • HAO Dahai ,
  • HUANG Zunxi ,
  • TANG Xianghua
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  • 1(College of Life Sciences, Yunnan Normal University, Kunming 650500, China)
    2(Yunnan Key Laboratory of Potato Biology, Kunming 650500, China)
    3(Key Lab of Yunnan Province for Biomass Energy and Environmental Biotechnology, Kunming 650500, China)

Received date: 2022-04-18

  Revised date: 2022-05-29

  Online published: 2023-04-14

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Abstract

In the BIOLOG system, 75 kinds of carbon sources were used for Eurotium cristatum SP-5 to screen the new enzyme species. The available carbon sources were screened out through chromogenic reactions. The polymeric substrates were designed based on the carbon source structure, which could be used in the induction culture of E. cristatum for directing enzyme production. E. cristatum SP-5 was used for solid-state fermentation to produce pectinase. The results showed that pectin, xylan, and cellulose were used as carbon sources to culture SP-5, and its colony morphology grew well. Based on the pectinase induced by SP-5 with pectin, solid-state fermentation was used to produce the enzyme by SP-5 with Pu-erh big leaf tea of Yunnan, and its enzymatic dynamics were analyzed. The experiments showed that E. cristatum SP-5 grew best in the pectin medium and had the highest pectinase activity in tea medium with 20% water content, reaching 12.5 U/g. The optimum reaction temperature and pH of pectinase from SP-5 were 50 ℃ and 3.5, respectively. The half-life of pectinase's heat and acid resistance was 30.28 min at 80 ℃ and 15.3 min at pH 2.0. The Km of SP-5 pectinase was 1.21 mg/mL, and the maximum reaction rate vmax was 6.15 μmol/(U·min). Metal ion magnesium, copper, iron, calcium, potassium, and silver ions have inhibitory effects on pectinase.

Key words： BIOLOG system; Eurotium cristatum; pectinase; dynamics; half-life

Cite this article

YUE Yanchao , YANG Yunjuan , HAO Dahai , HUANG Zunxi , TANG Xianghua . Solid-state fermentation to Pu-erh big leaf tea with Eurotium cristatum SP-5 to produce pectinase[J]. Food and Fermentation Industries, 2023 , 49(6) : 162 -169 . DOI: 10.13995/j.cnki.11-1802/ts.031971

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