In order to understand the contamination status, virulence gene profile and antimicrobial resistance of Cronobacter spp. in imported food from Xishuangbanna, Yunnan, this study used VITEK 2 compact system and 16S rDNA sequencing to isolate and identify Cronobacter spp., amplified four virulence genes by PCR, and performed the antibiotic susceptibility test of 14 antibiotics by Kirby Bauer Method. And whole genome sequencing was performed for strain BQ10. The results showed that 2.91% (5/172) of the samples were contaminated with Cronobacter spp., and the carriage rate of virulence genes ompX, cpa, and hly was 100%, and none of the sip was detected. isolates were most resistant to cefothiophene (CFT), with a resistance rate of 69.23% (9/13), they were generally sensitive to antibiotics such as ampicillin, amoxicillin, ticarcillin, imipenem, and ciprofloxacin, and no multi-antibiotic resistant strains were found. Whole genome sequencing showed that the genome size of BQ10 was 4.75 Mb, the GC content was 54.69%, and the sequence type was ST431. A variety of virulence genes and antibiotic resistance genes were predicted by VFDB and CARD. The results of this study provide reference for the risk assessment, early warning and relevant prevention and control measures of foodborne pathogens in imported food from Xishuangbanna.
ZHANG Xi
,
CHEN Yun
,
LYU Tingbao
,
GUAN Dingru
,
LI Wei
,
YANG Lingchun
,
ZHU Renjun
,
HU Yongjin
. Identification, virulence genes, and antibiotic resistance of Cronobacter spp. in imported food from Xishuangbanna, Yunnan[J]. Food and Fermentation Industries, 2023
, 49(6)
: 186
-192
.
DOI: 10.13995/j.cnki.11-1802/ts.031880
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