Food and Fermentation Industries >

2023 , Vol. 49 >Issue 7: 16 - 25

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032969

Biosynthesis pathway of cordycepin in Cordyceps militaris based on metabonomics

  • LIU Guijun ,
  • QIAO Yuchen ,
  • ZHOU Sijing ,
  • WANG Ping ,
  • SONG Meifang ,
  • YANG Suling ,
  • GU Haike ,
  • HOU Li
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  • (Institute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, China)

Received date: 2022-07-13

  Revised date: 2022-08-18

  Online published: 2023-04-28

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Abstract

In this study, the cordycepin biosynthesis pathway was studied by analyzing the metabolome data of Cordyceps militaris strains. The starting strain CM08, the high-yield cordycepin positive mutant strain CM09 and the low-yield cordycepin negative mutant strain CM10 obtained by radiation mutagenesis were studied. The untargeted metabolomics data of CM08, CM09 ,and CM10 were determine by ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF/MS), and multivariate statistical analysis was conducted. The results of principal component analysis showed that there were significant differences among metabolites of CM08, CM09, and CM10. Differential metabolites were distinguished by variable importance for the projection (VIP) obtained by orthogonal partial least squares discrimination analysis model (VIP>1 and P<0.05), and there were 64 differential metabolites ofCM09 vs CM08 and 147 differential metabolites of CM10 vs CM08, including amino acids, saccharides and nucleoside compounds. Enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways showed that differential metabolites were significantly enriched in purine metabolic pathway, pentose phosphate pathway and ABC transporter pathway. The relative contents down-regulated and up-regulated of enriched differential metabolites adenosine, ADP-ribose, 2′-deoxyadenosine, adenosine monophosphate (AMP), 3′-AMP and cordycepin were reversed in positive and negative mutant strains, which showed that these differential metabolites were closely related to the yield of cordycepin, Combined with literature reports and the results of this study, the biosynthesis pathway of cordycepin is speculated as follows: ADP-Ribose→Ribose-5-P→PRPP→IMP→Adenylosuccinate→AMP→Adenosine→3′-AMP→2′-C-3′-dA→cordycepin, which provides important basis for the study of cordycepin biosynthesis pathway.

Key words： Cordyceps militaris; cordycepin; metabolomics; differential metabolites; biosynthesis pathway

Cite this article

LIU Guijun , QIAO Yuchen , ZHOU Sijing , WANG Ping , SONG Meifang , YANG Suling , GU Haike , HOU Li . Biosynthesis pathway of cordycepin in Cordyceps militaris based on metabonomics[J]. Food and Fermentation Industries, 2023 , 49(7) : 16 -25 . DOI: 10.13995/j.cnki.11-1802/ts.032969

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