Lacticaseibacillus paracasei PC-01 is a potential probiotic candidate isolated from naturally fermented yak milk in Lhasa, Tibet, China. In this study, we investigated the effect of different doses of strain PC-01 on the recovery of dextran sulphate sodium-induced colitis in a mouse model. The survival rate, body weight, disease activity index score, colon length, tissue sections, serum concentrations of myeloperoxidase and cytokines, and fecal lactate and short-chain fatty acid contents of mice were measured during the test period, and the effects of different doses of strain PC-01 on the recovery of colitis were evaluated. The results showed that a high dose of strain PC-01(1×109 CFU/d) increased the survival rate, accelerated the body weight gain, significantly improved the fecal morphology and bloody stool, promoted the recovery of colonic tissue lesions in mice, reduced the extent of inflammation, lowered the serum concentrations of myeloperoxidase and pro-inflammatory factors IL-1β and IL-6, and significantly increased the fecal contents of lactic acid and short-chain fatty acids. Our data confirmed that a high- PC-01 dose had the most significant effect on the recovery of colitis in mice.
[1] RAMOS G P, PAPADAKIS K A.Mechanisms of disease:Inflammatory bowel diseases[J].Mayo Clinic Proceedings, 2019, 94(1):155-165.
[2] KHAN I, ULLAH N, ZHA L J, et al.Alteration of gut microbiota in inflammatory bowel disease (IBD):Cause or consequence? IBD treatment targeting the gut microbiome[J].Pathogens (Basel, Switzerland), 2019, 8(3):126.
[3] 杨勇, 刘作义.炎症性肠病[J].中国临床医生, 2009, 37(2):21-24.
YANG Y, LIU Z Y.Inflammatory bowel disease[J].Chinese Journal for Clinicians, 2009, 37(2):21-24.
[4] FRIEDRICH M, POHIN M, POWRIE F.Cytokine networks in the pathophysiology of inflammatory bowel disease[J].Immunity, 2019, 50(4):992-1 006.
[5] NEURATH M F.Cytokines in inflammatory bowel disease[J].Nature Reviews Immunology, 2014, 14(5):329-342.
[6] ERREA A, CAYET D, MARCHETTI P, et al.Lactate inhibits the pro-inflammatory response and metabolic reprogramming in murine macrophages in a GPR81-independent manner[J].PLoS One, 2016, 11(11):e0163694.
[7] RANGANATHAN P, SHANMUGAM A, SWAFFORD D, et al.GPR81, a cell-surface receptor for lactate, regulates intestinal homeostasis and protects mice from experimental colitis[J].Journal of Immunology (Baltimore, Md.:1950), 2018, 200(5):1 781-1 789.
[8] PARADA VENEGAS D, DE LA FUENTE M K, LANDSKRON G, et al.Short chain fatty acids (SCFAs)-mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases[J].Frontiers in Immunology, 2019, 10:277.
[9] ZHUANG X J, LI T, LI M Y, et al.Systematic review and meta-analysis:Short-chain fatty acid characterization in patients with inflammatory bowel disease[J].Inflammatory Bowel Diseases, 2019, 25(11):1 751-1 763.
[10] 田春雨. 乳酸杆菌对DSS-诱导的小鼠溃疡性结肠炎的抑制作用[D].哈尔滨:哈尔滨工业大学, 2018.
TIAN C Y.Lactobacillus inhibits dss-induced ulcerative colitis in mice[D].Harbin:Harbin Institute of Technology, 2018.
[11] CHEN C L, HSU P Y, PAN T M.Therapeutic effects of Lactobacillus paracasei subsp.paracasei NTU 101 powder on dextran sulfate sodium-induced colitis in mice[J].Journal of Food and Drug Analysis, 2019, 27(1):83-92.
[12] HUANG J, YANG Z Y, LI Y Y, et al.Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance[J].Journal of Translational Medicine, 2021, 19(1):356.
[13] 刘凯龙, 康小红, 张哲, 等.副干酪乳杆菌PC-01益生特性和安全性研究[J].中国食品学报, 2021, 21(11):47-52.
LIU K L, KANG X H, ZHANG Z, et al.Studies on probiotic properties and safety of Lactobacillus paracasei PC-01[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(11):47-52.
[14] MURANO M, MAEMURA K, HIRATA I, et al.Therapeutic effect of intracolonically administered nuclear factor κB (p65) antisense oligonucleotide on mouse dextran sulphate sodium (DSS)-induced colitis[J].Clinical and Experimental Immunology, 2001, 120(1):51-58.
[15] 王俊通. 产共轭亚油酸乳酸菌对DSS诱导小鼠结肠炎的改善作用[D].无锡:江南大学, 2016.
WANG J T.Ameliorating effects of CLA-producing lactic acid bacteria on DSS-induced colitis in mice[D].Wuxi:Jiangnan University, 2016.
[16] 蒿雨. 植物乳杆菌P9对农药暴露人群粪便胆汁酸和短链脂肪酸代谢的影响[D].呼和浩特:内蒙古农业大学, 2021.
HAO Y.Effect of Lactobacillus plantarum P9 on fecal bile acid and short-chain fatty acids metabolism of populations exposed to pesticide[D].Hohhot:Inner Mongolia Agricultural University, 2021.
[17] KIM D H, KIM S, AHN J B, et al.Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice[J].International Journal of Medical Microbiology, 2020, 310(2):151391.
[18] PAN T, GUO H Y, ZHANG H, et al.Oral administration of Lactobacillus paracasei alleviates clinical symptoms of colitis induced by dextran sulphate sodium salt in BALB/c mice[J].Beneficial Microbes, 2014, 5(3):315-322.
[19] 吴思琪. 植物乳杆菌Zhang-LL对DSS诱导大鼠慢性溃疡性结肠炎预防效果评价[D].北京:北京农学院, 2018.
WU S Q.Effect on DSS-induced chronic ulcerative colitis in rats of Lactobacillus plantarum Zhang-LL[D].Beijing:Beijing University of Agriculture, 2018.
[20] CHAMI B, MARTIN N J J, DENNIS J M, et al.Myeloperoxidase in the inflamed colon:A novel target for treating inflammatory bowel disease[J].Archives of Biochemistry and Biophysics, 2018, 645:61-71.
[21] BOUGHTON-SMITH N K, WALLACE J L, WHITLE B J R.Relationship between arachidonic acid metabolism, myeloperoxidase activity and leukocyte infiltration in a rat model of inflammatory bowel disease[J].Agents and Actions, 1988, 25(1):115-123.
[22] HANSBERRY D R, SHAH K, AGARWAL P, et al.Fecal myeloperoxidase as a biomarker for inflammatory bowel disease[J].Cureus, 2017, 9(1):e1004.
[23] 王碧莹. 四株乳酸杆菌对溃疡性肠道炎症的调节作用研究[D].哈尔滨:哈尔滨工业大学, 2017.
WANG B Y.Effect of four strains of Lactobacillus on ulcerative intestinal inflammation[D].Harbin:Harbin Institute of Technology, 2017.
[24] MUDTER J, NEURATH M F.Il-6 signaling in inflammatory bowel disease:Pathophysiological role and clinical relevance[J].Inflammatory Bowel Diseases, 2007, 13(8):1 016-1 023.
[25] ROERS A, SIEWE L, STRITTMATTER E, et al.T cell-specific inactivation of the interleukin 10 gene in mice results in enhanced T cell responses but normal innate responses to lipopolysaccharide or skin irritation[J].The Journal of Experimental Medicine, 2004, 200(10):1 289-1 297.
[26] GABRYŠOVÁ L, HOWES A, SARAIVA M, et al. Interleukin-10 in Health and Disease.Berlin[M]. Heidelberg:Springer, 2014:157-190.
[27] WATANABE T, NISHIO H, TANIGAWA T, et al.Probiotic Lactobacillus casei strain Shirota prevents indomethacin-induced small intestinal injury:Involvement of lactic acid[J].American Journal of Physiology.Gastrointestinal and Liver Physiology, 2009, 297(3):G506-G513.
[28] 王彦博. 功能各异的益生菌复合缓解DSS诱导的溃疡性结肠炎机制研究[D].长春:吉林大学, 2019.
WANG Y B.Study on the mechanism of alleviating DSS-induced ulcerative colitis by the combination of probiotics with different functions[D].Changchun:Jilin University, 2019.
[29] 郑雨星, 朱慧越, 焦婷, 等.鼠李糖乳杆菌FZJTZ46L6通过影响肠道内短链脂肪酸的水平缓解小鼠由葡聚糖硫酸钠导致的结肠炎[J].食品与发酵工业, 2020, 46(10):44-52.
ZHENG Y X, ZHU H Y, JIAO T, et al.Lactobacillus rhamnosus FZJTZ46L6 alleviates colitis induced by dextran sulfate sodium in mice through short-chain fatty acids regulation[J].Food and Fermentation Industries, 2020, 46(10):44-52.
