Food and Fermentation Industries >

2023 , Vol. 49 >Issue 7: 66 - 72

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031731

In vitro study on the effect of baicalein combined with nisin on biofilm of Staphylococcus aureus

  • WANG Zhao ,
  • ZHU Zekang ,
  • NIE Rong ,
  • LIU Guorong
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  • (Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China)

Received date: 2022-04-22

  Revised date: 2022-05-02

  Online published: 2023-04-28

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Abstract

The biofilm of Staphylococcus aureus is an important reason for its proliferation and toxin production. This paper explores the combined use of nisin and baicalein to enhance the inhibitory effect on S. aureus biofilm. It’s also screened and determined the inhibitory effect and optimal concentration ratio of nisin combined with traditional Chinese medicine ingredients on biofilm, and explored the mechanism of action in inhibiting biofilm formation. The results showed that the MIC50 values of nisin and baicalein were 0.5 and 0.1 mg/mL, respectively. These multiple concentration ratios have synergistic inhibitory effect on biofilm and obtain content anti-biofilm effect with lower dose. The optimal concentration combination was 0.062 5 mg/mL nisin and 3.125 μg/mL baicalein. This combination also reduced the adhesion, hydrophobic effect, autofocusing ability, extracellular polysaccharide ,and eDNA production of biofilm, indicating that they could inhibit the adhesion, growth ,and maturation of biofilm. At 18 h, the inhibition and scavenging effect on biofilm was better than others. Scanning electron microscope results showed that the biofilm attachment was greatly reduced and the colonies were distinct. In conclusion, the combination of nisin and baicalein can synergistically inhibit the formation of S. aureus biofilm, further establish the foundation for the development of more efficient natural food preservative.

Key words： nisin; baicalein; Staphylococcus aureus; bacterial biofilm; obtain content anti-biofilm effect with lower dose

Cite this article

WANG Zhao , ZHU Zekang , NIE Rong , LIU Guorong . In vitro study on the effect of baicalein combined with nisin on biofilm of Staphylococcus aureus[J]. Food and Fermentation Industries, 2023 , 49(7) : 66 -72 . DOI: 10.13995/j.cnki.11-1802/ts.031731

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