Aerobic treatment is an effective method for kitchen waste treatment. In this study, six strains with high degrading rate were screened, namely BLE, BI1, BI2, LZM-B, B10-5, and WL-BA-1) that can efficiently degrade more than 90% of the solids of kitchen waste in 20 h from a library of high enzyme-producing bacteria kept in the laboratory by simulating the degradation process of biochemical treatment with the help of shaking flasks with constant temperature and shaking culture. BLE and BI2 strains can efficiently degrade more than 90% of kitchen waste containing 5.0% salt and 6.0% oil within 20 h. In addition, the strains BLE and BI2 can still degrade the kitchen waste in 12 consecutive batches, and the degradation rate of both of them is higher than 90%. By molecular biological identification, BLE and BI2 strains were identified as Bacillus amyloliquefaciens. Finally, the hydrolytic enzymes produced by BLE and BI2 strains at different times were analyzed, and it was found that both strains reached the peak of enzyme production within 24 h of fermentation, which is an important reason for their rapid and efficient degradation of kitchen waste, and also provided technical support for the rapid reduction of kitchen waste.
[1] 国家统计局. 中国统计摘要-2020[M].北京:中国统计出版社, 2020.
National Bureau of Statistics.China Statistical Yearbook-2020[M].Beijing:China Statistics Press, 2020.
[2] 赵振振, 张红亮, 殷俊, 等.对我国城市生活垃圾分类的分析及思考[J].资源节约与环保, 2021(8):128-131.
ZHAO Z Z, ZHANG H L, YIN J, et al.Analysis and thinking on the classification of municipal solid waste in China[J].Resources Economization & Environmental Protection, 2021(8):128-131.
[3] 余培斌,吴殿辉,蔡国林,等.餐厨垃圾发酵产乳酸菌种的筛选、鉴定及应用[J].食品与发酵工业,2023,49(3):53-59.
YU P B, WU D H, CAI G L, et al. Screening, identification and application of lactic acid producing bacteria from food waste fermentation[J].Food and Fermentation Industry, 2023,49(3):53-59.
[4] WU M K, HU J G, SHEN F, et al.Conceptually integrating a multi-product strategy for the valorization of kitchen waste towards a more sustainable management[J].Journal of Cleaner Production, 2021, 306:127292.
[5] 袁彧, 陆新生.垃圾分类政策实施后国内厨余垃圾性质及处置现状研究[J].可持续发展, 2021(3):348-354.
YUAN Y, LU X.Research on the characteristic and disposal status of domestic kitchen waste after the implementation of the waste classification policy[J].Sustainable Development, 2021(3):348-354.
[6] 崔文静, 陆敏博.餐厨垃圾处理现状及今后发展趋势[J].广东化工, 2021, 48(19):140-141.
CUI W J, LU M B.The present disposing situation and development trend of kitchen waste[J].Guangdong Chemical Industry, 2021, 48(19):140-141.
[7] 咸芳. 餐厨垃圾高效处理复合微生物菌剂的研究[D].长春:吉林大学, 2009.
XIAN F.Research on effective microorganism agent for degradation of food wastes[D].Changchun:Jilin University, 2009.
[8] 阚丹, 司马小峰.我国餐厨垃圾分类现状及处理技术展望[J].环境保护前沿, 2020(1):98-105.
DAN D, SIMA X F.Classification status and technology outlook of kitchen waste in China[J].Advances in Environmental Protection, 2020(1):98-105.
[9] LU D, ZHOU Z.Discussion on intelligent disposal system of high-fat food waste[J].Journal of Physics:Conference Series, 2021, 1 852(2):022001.
[10] 郝鑫, 苏婧, 孙源媛, 等.餐厨垃圾与污泥、秸秆不同配比联合厌氧发酵对产气性能的影响[J].环境科学研究, 2020, 33(1):235-242.
HAO X, SU J, SUN Y Y, et al.Biogas production of anaerobic Co-digestion with different ratios of kitchen waste, sewage sludge and rice straw[J].Research of Environmental Sciences, 2020, 33(1):235-242.
[11] 赵紫萱, 邱卫华, 王攀.餐厨垃圾营养复配及其在复合微生物菌剂作用下的好氧降解[J].环境工程, 2021, 39(4):92-99.
ZHAO Z X, QIU W H, WANG P.The aerobic degradation of nutritional complexed kitchen waste by mixed microbial flora[J].Environmental Engineering, 2021, 39(4):92-99.
[12] 吉雨霁, 王娜, 杨宁, 等.餐厨垃圾高温好氧生物减量菌种的筛选及特性[J].微生物学通报, 2022, 49(11):4 513-4 524.
JI Y J, WANG N, YANG N, et al.Screening of bacteria for thermophilic aerobic biodegradation of food waste and the degradation characteristics[J].Microbiology China, 2022, 49(11):4 513-4 524.
[13] 时朝辉. 餐厨垃圾微生物减量化处理研究[D].上海:华东师范大学, 2017.
SHI C H.Research on minimizing kitchen waste with microbial technology[D].Shanghai:East China Normal University, 2017.
[14] 张雪娇, 田欢, 刘春叶, 等.可见分光光度法测定α-淀粉酶活力[J].化学与生物工程, 2020, 37(3):65-68.
ZHANG X J, TIAN H, LIU C Y, et al.Determination of the activity of α-amylase by visible spectrophotometry[J].Chemistry & Bioengineering, 2020, 37(3):65-68.
[15] 刘永康. 碱性蛋白酶高产菌株的诱变选育[D].洛阳:河南科技大学, 2020.
LIU Y K.Mutagenesis and breeding of alkaline protease high-yield strain[D].Luoyang:Henan University of Science and Technology, 2020.
[16] 许燕博. 中间苍白杆菌产生物乳化剂的发酵条件及产物分离[D].大连:大连工业大学, 2016.
XU Y B.Fermentation conditions and separation of bioemulsifier from Ochrobactrum intermedium[D].Dalian:Dalian Polytechnic University, 2016.
[17] 李颖, 王德培, 王勇, 等.一种简单准确的酵母微量活菌计数方法[J].中国酿造, 2008, 27(18):75-77.
LI Y, WANG D P, WANG Y, et al.A new method for counting living yeast cell number[J].China Brewing, 2008, 27(18):75-77.
[18] 李江东. 餐厨垃圾好氧堆肥资源化利用及无害化处理的研究[D].南昌:南昌大学, 2021.
LI J D.Research on resource utilization and harmless treatment of food waste[D].Nanchang:Nanchang University, 2021.
[19] DEN B J, GUDRUN O, SEBASTIAN G, et al.Enhancement of food waste management and its environmental consequences[J].Energies, 2021, 14(6):1790.
[20] ZENG Z T, LIU Y, ZHONG H, et al.Mechanisms for rhamnolipids-mediated biodegradation of hydrophobic organic compounds[J].Science of the Total Environment, 2018, 634:1-11.
[21] 程春平. 餐厨垃圾油脂降解菌的筛选及其应用[D].合肥:安徽农业大学, 2020.
CHENG C P.Screening and application for oil degradation bacteria in food waste[D].Hefei:Anhui Agricultural University, 2020.
[22] 胡新军, 张敏, 余俊锋, 等.中国餐厨垃圾处理的现状、问题和对策[J].生态学报, 2012, 32(14):4 575-4 584.
HU X J, ZHANG M, YU J F, et al.Food waste management in China:Status, problems and solutions[J].Acta Ecologica Sinica, 2012, 32(14):4 575-4 584.
[23] 邓蒙轩, 周爱娟, 万方, 等.优化餐厨垃圾乳化液深度去油方案[J].科学技术与工程, 2020, 20(4):1 704-1 710.
DENG M X, ZHOU A J, WAN F, et al.Optimization of deeply oil removal method from food residue emulsion[J].Science Technology and Engineering, 2020, 20(4):1 704-1 710.
[24] 张志波. 表面活性剂对堆肥中微生物产酶及有机质降解的影响研究[D].长沙:湖南大学, 2007.
ZHANG Z B.Effects on the production of enzymes from microorganisms and biodegradation by surfactants[D].Changsha:Hunan University, 2007.
[25] 刘婕. 除油菌的筛选鉴定及油脂废水处理研究[D].广州:华南理工大学, 2010.
LIU J.Study on screening and identification of oil degradated strains and treatment of oil wastewater[D].Guangzhou:South China University of Technology, 2010.
[26] 何晓铮. 盐分对餐厨垃圾厌氧产酸发酵的影响研究[D].杭州:浙江工商大学, 2020.
HE X Z.Study on the effect of salt on acidogenic fermentation of food waste[D].Hangzhou:Zhejiang Gongshang University, 2020.
