Food and Fermentation Industries >

2023 , Vol. 49 >Issue 7: 153 - 158

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032302

The Cd chelating capability of siderophores secreted by microorganisms was identified via a CAS-Cd plate covering

  • GUAN Wenhao ,
  • WANG Aowei ,
  • FANG Zhijia ,
  • SUN Lijun ,
  • LIU Ying ,
  • LIAO Jianmeng
Expand
  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)
    3(Zhanjiang Institute of Food and Drug Control, Zhanjiang 524088, China)

Received date: 2022-05-11

  Revised date: 2022-05-28

  Online published: 2023-04-28

Fold

Abstract

In order to more intuitively and stably identify the ability of microorganisms to secrete siderophores for chelating cadmium, CAS-Fe (Ⅲ) plate culture method was used to confirm that Burkholderia cepacia had the ability to secrete siderophores, and CAS-Cd (Ⅱ) detection solution was modified based on the working principle of CAS-Fe (Ⅲ) detection solution. On the basis of CAS-Cd (Ⅱ) detection solution, bacterial supernatant detection method, plate method and plate covering method were used to identify whether the siderophore secreted by the bacterium had the ability of chelating cadmium. The results showed that B. cepacia had the ability to secrete siderophore. The liquid detection method had interference factors, and the plate method could not detect or culture bacteria, but the plate covering method could ensure the growth of microorganisms, and obvious fading circles due to cadmium chelation were observed around the bacterial colonies after 12 h treatment. It was suggested that the CAS-Cd (Ⅱ) plate covering method could clearly and stably identify the ability of chelating cadmium in microbes. This study verified the effect of siderophores secreted by microorganisms on chelating cadmium, providing a new method and idea for screening microorganisms to chelate heavy metals.

Key words： cadmium; Burkholderia cepacian; siderophore; CAS-Cd (Ⅱ) detection solution

Cite this article

GUAN Wenhao , WANG Aowei , FANG Zhijia , SUN Lijun , LIU Ying , LIAO Jianmeng . The Cd chelating capability of siderophores secreted by microorganisms was identified via a CAS-Cd plate covering[J]. Food and Fermentation Industries, 2023 , 49(7) : 153 -158 . DOI: 10.13995/j.cnki.11-1802/ts.032302

References

[1] 曾思燕, 于昊辰, 马静, 等.中国耕地表层土壤重金属污染状况评判及休耕空间权衡[J].土壤学报, 2022, 59(4):1 036-1 047.
ZENG S Y, YU H C, MA J, et al.Identifying the status of heavy metal pollution of cultivated land for tradeoff spatial fallow in China[J].Acta Pedologica Sinica, 2022, 59(4):1 036-1 047.
[2] KAMAL S, PRASAD R, VARMA A.Soil microbial diversity in relation to heavy metals[M]//Soil Biology.Berlin, Heidelberg:Springer Berlin Heidelberg, 2009:31-63.
[3] IZRAEL-ŽIVKOVIĆ L, RIKALOVIĆ M, GOJGIĆ-CVIJOVIĆ G, et al.Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa San ai[J].RSC Advances, 2018, 8(19):10 549-10 560.
[4] 张笑雨, 朱建明, 蔡中华, 等.海洋微生物铁载体的研究进展[J].生物化学与生物物理进展, 2022, 49(9):1 658-1 671.
ZHANG X Y, ZHU J M, CAI Z H, et al.The research advance of siderophores in marine microbes[J].Progress in Biochemistry and Biophysics,2022, 49(9):1 658-1 671.
[5] HESSE E, O’BRIEN S, TROMAS N, et al.Ecological selection of siderophore-producing microbial taxa in response to heavy metal contamination[J].Ecology Letters, 2018, 21(1):117-127.
[6] BHATTACHARYA A.Siderophore mediated metal uptake by Pseudomonas fluorescens and its comparison to Iron (III) chelation[J].Ceylon Journal of Science (Biological Sciences), 2011, 39(2):147.
[7] KURUCZ V, KISS B, SZIGETI Z M, et al.Physiological background of the remarkably high Cd2+ tolerance of the Aspergillus fumigatus Af293 strain [J].Journal of Basic Microbiology, 2018, 58(11):957-967.
[8] SINHA S, MUKHERJEE S K.Cadmium-induced siderophore production by a high Cd-resistant bacterial strain relieved Cd toxicity in plants through root colonization [J].Current Microbiology, 2008, 56(1):55-60.
[9] SCHWYN B, NEILANDS J B.Universal chemical assay for the detection and determination of siderophores[J].Analytical Biochemistry, 1987, 160(1):47-56.
[10] 慈云祥, 杨明敏.多元络合物显色反应的研究Ⅸ镉(Ⅱ)-铬天青S-2, 2’-联吡啶-溴化十六烷基吡啶体系[J].化学试剂, 1982, 4(4):209-213;257.
CI Y X, YANG M M.Studies on the colour reactions of multicom ponent complexes Ⅸ.Cadmium (Ⅱ)-chromeazurol S-2, 2’-dipyridine-cetylpyridinium bromide system[J].Chemical Reagents, 1982, 4(4):209-213;257.
[11] 王迪民. 阴离子交换分离—CAS—Phen—CTMAB分光光度法测定闪锌矿中微量镉[J].冶金分析, 1986, 6(2):26-29.
WANG D M.Spectrophotometric determination of micro amount of cadmium in blende with O-phenanthroline-chromazurol S-cetyltrimethylammonlum bromide by anion exck-ange separation[J].Metallurgical Analysis, 1986, 6(2):26-29.
[12] MEESUNGNOEN O, CHANTIRATIKUL P, THUMANU K, et al.Elucidation of crude siderophore extracts from supernatants of Pseudomonas sp.ZnCd2003 cultivated in nutrient broth supplemented with Zn, Cd, and Zn plus Cd [J].Archives of Microbiology, 2021, 203(6):2 863-2 874.
[13] SCHWABE R, ANKE M K, SZYMAŃSKA K, et al.Analysis of desferrioxamine-like siderophores and their capability to selectively bind metals and metalloids:Development of a robust analytical RP-HPLC method[J].Research in Microbiology, 2018, 169(10):598-607.
[14] MULAR A, SHANZER A, KOZĿOWSKI H, et al.Cyclic analogs of desferrioxamine E siderophore for68Ga nuclear imaging:coordination chemistry and biological activity in Staphylococcus aureus [J].Inorganic Chemistry, 2021, 60(23):1 7 846-1 7857.
[15] BRAVO D, BRAISSANT O.Cadmium-tolerant bacteria:Current trends and applications in agriculture [J].Letters in Applied Microbiology, 2022, 74(3):311-333.
[16] ZHANG J H, LI Q, ZENG Y F, et al.Bioaccumulation and distribution of cadmium by Burkholderia cepacia GYP1 under oligotrophic condition and mechanism analysis at proteome level[J].Ecotoxicology and Environmental Safety, 2019, 176:162-169.
[17] HUANG W, LI Y Q, et al.Isolation, characterization, and evaluation of a high-siderophore-yielding bacterium from heavy metal-contaminated soil[J].Environmental Science and Pollution Research, 2022, 29(3):3 888-3 899.
[18] 许佳露, 张平, 李美芳, 等.产铁载体菌株的分离、培养条件优化及初步应用[J].微生物学通报, 2022, 49(3):1 004-1 016.
XU J L, ZHANG P, LI M F, et al.Isolation, culture condition optimization, and preliminary application of siderophore-producing strains[J].Microbiology China, 2022, 49(3):1 004-1 016.
[19] 王东升, 王立立, 李取生, 等.产铁载体菌对龙葵修复土壤Cd污染的促进效应[J].环境工程学报, 2018, 12(8):2 311-2 319.
WANG D S, WANG L L, LI Q S, et al.Enhancing effect of siderophore-producting bacteria on remediation of cadmium-contaminated soil by Solanum nigrum L [J].Chinese Journal of Environmental Engineering, 2018, 12(8):2 311-2 319.
[20] CUNNINGHAM T M, KOEHL J L, SUMMERS J S, et al.pH-dependent metal ion toxicity influences the antibacterial activity of two natural mineral mixtures [J].PLoS One, 2010, 5(3):e9456.
[21] PAYNE S M.Detection, isolation, and characterization of siderophores [J].Methods in Enzymology, 1994, 235:329-344.
[22] LUTTER E, LEWENZA S, DENNIS J J, et al.Distribution of quorum-sensing genes in the Burkholderia cepacia complex[J].Infection and Immunity, 2001, 69(7):4 661-4 666.
[23] PISHCHANY G, HALEY K P, SKAAR E P.Staphylococcus aureus growth using human hemoglobin as an iron source[J].Journal of Visualized Experiments:JoVE, 2013(72):50072.
[24] MYERS G E, PYRCH D.Residual bactericidal activity of cetyl pyridinium bromide (Fixanol C) against Staphylococcus aureus [J].The American Journal of the Medical Sciences, 1962, 243:475-479.
[25] MILAGRES A M F, MACHUCA A, NAPOLEÃO D.Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay[J].Journal of Microbiological Methods, 1999, 37(1):1-6.
[26] 王卫星, 周晓伦, 李忠玲, 等.CAS平板覆盖法检测氢氧化细菌铁载体 [J].微生物学通报, 2014, 41(8):1 692-1 697.
WANG W X, ZHOU X L, LI Z L, et al.Detection of siderophore production from hydrogen-oxidizing bacteria with CAS overlay plate method [J].Microbiology China, 2014, 41(8):1 692-1 697.
[27] ALI S S, VIDHALE N N.Bacterial siderohore and their application:A review [J].International Journal of Current Microbiology and Applied Sciences, 2013, 2(12):303-312.
[28] GHOSH S K, BERA T, CHAKRABARTY A M.Microbial siderophore-A boon to agricultural sciences[J].Biological Control, 2020, 144:104214.
[29] 魏本杰, 曾晓希, 刘志成, 等.产铁载体菌的筛选鉴定及活化镉的性能探究[J].环境科学与技术, 2014, 37(11):26-31.
WEI B J, ZENG X X, LIU Z C, et al.Isolation, identification and study on cadmium-mobilization ability of sidorophores-producing bacteria[J].Environmental Science & Technology, 2014, 37(11):26-31.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.