Food and Fermentation Industries >

2023 , Vol. 49 >Issue 7: 174 - 180

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032219

Preparation of a high purity small molecule anti-alcoholic liver protecting peptide

  • WEN Qingshi ,
  • WEI Hefen ,
  • ZHOU Jingwei ,
  • YING Hanjie ,
  • CHEN Yong ,
  • LIU Qingguo ,
  • YUAN Tong ,
  • LIU Miao ,
  • SHEN Caihong ,
  • WANG Songtao
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  • 1(Jiangsu Jicui Industrial Biotechnology Research Institute Co.Ltd., Nanjing 211800, China)
    2(Nanjing Hi Tech University Biotechnology Research Institute Co.Ltd.,Nanjing 211800, China)
    3(National Biochemical Engineering Technology Research Center, Nanjing University of Technology, Nanjing 210000, China)
    4(Luzhou Laojiao Co.Ltd., Luzhou 646000, China)

Received date: 2022-05-05

  Revised date: 2022-06-23

  Online published: 2023-04-28

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Abstract

At present, the research of marine bioactive peptides in functional foods has become popular. To solve the problem of the deficiency in traditional Chinese and Western anti-alcoholics, a small molecule anti-alcoholic liver protecting peptide with high purity and activity was prepared in this study. Squid protein was moderately hydrolyzed by multiple enzymes, and the protein recovery rate and alcohol dehydrogenase (ADH) activation rate were used as indicators. The optimum enzymatic hydrolysis conditions were∶pepsin∶alkaline protease∶flavor protease=1∶1∶1, enzyme addition 0.2% (mass fraction), solid-liquid ratio 1∶10 (g∶mL) and reaction time 3 h. Under the optimum conditions, the protein recovery rate was 80.34% and the ADH activation rate was 83.37%. In order to further purify the small peptide and improve its functional activity, the refining process of the enzymatic hydrolysate was studied. The hydrophobic peptide components were adsorbed by resin, eluted with gradient ethanol, and the collected solution was concentrated and dried to obtain a light yellow peptide powder. The purity of its protein peptide was 95.33%, peptide with the molecular weight under 3 000 Da was 99.96%, the ADH activation ratio was 122.71%, and the scavenging rate of DPPH free radical was 92.01%. The small peptide products prepared in this study have great application potential in the field of functional food.

Key words： moderate enzyme-complex hydrolysis; small molecule peptide; ADH activation rate; DPPH free radicals; protect liver therapy

Cite this article

WEN Qingshi , WEI Hefen , ZHOU Jingwei , YING Hanjie , CHEN Yong , LIU Qingguo , YUAN Tong , LIU Miao , SHEN Caihong , WANG Songtao . Preparation of a high purity small molecule anti-alcoholic liver protecting peptide[J]. Food and Fermentation Industries, 2023 , 49(7) : 174 -180 . DOI: 10.13995/j.cnki.11-1802/ts.032219

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