Food and Fermentation Industries >

2023 , Vol. 49 >Issue 8: 170 - 176

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032703

Screening, identification, and toxin-producing ability analysis of aflatoxin producing strains in brine of Xiangpai brined products

  • LIN Lidan ,
  • ZHAO Liangzhong ,
  • HUANG Zhanrui ,
  • ZHOU Xiaojie ,
  • AI Daoying ,
  • ZHOU Hengping ,
  • ZHOU Jinsong ,
  • ZHENG Yirou ,
  • ZHOU Kai
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  • 1(College of Food and Chemical Engineering, Shaoyang University, Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Shaoyang 422000, China)
    2(Pingjiang jinzai Food Co.Ltd., Yueyang 414000, China)
    3(Shenzhen Total-Test Technology Co.Ltd., Guangdong Engineering Research Center of High Specificity Biological Rapid Detection, Shenzhen 518038, China)

Received date: 2022-06-16

  Revised date: 2022-07-09

  Online published: 2023-05-16

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Abstract

Taking the brine of Xiangpai brined products as the research object, the microorganisms producing aflatoxin (AFs) during storage were screened and identified, and the toxin-producing ability was determined. The plate dilution coating method and ultraviolet fluorescence method were used to screen the toxin-producing microorganism in Xiangpai brine. The ITS sequences of the preliminarily screened strains were amplified by PCR and sequenced. The sequencing results by NCBI homology comparison and the phylogenetic tree was constructed by MEGA6.0 software for molecular biological identification. HPLC-MS/MS was used to detect the content of AFs and to determine the toxin-producing ability of toxin-producing strains. Five toxin-producing strains were screened and identified by plate dilution coating method, ultraviolet fluorescence method, and molecular biology. Four strains were Aspergillus flavus and one strain was Aspergillus parasiticus. None of the four A. flavus strains produced AFs based on the result of HPLC-MS/MS, but the A. parasiticus produced AFB1 and AFG1. The contents of AFB1 on the 3rd and 7th days were 6.92 μg/L and 8.08 μg/L, respectively. The contents of AFG1 on the 3rd and 7th days were 5.80 μg/L and 7.24 μg/L, respectively. Aspergillus producing AFs exists in Xiangpai brine due to improper storage conditions, and it is vulnerable to the influence of moldy raw materials and environment, which warns the safety risk of AFs pollution in Xiangpai brined products.

Key words： brine; toxigenic bacteria; toxin-producing capability; aflatoxin; Aspergillus flavus; Aspergillus parasiticus

Cite this article

LIN Lidan , ZHAO Liangzhong , HUANG Zhanrui , ZHOU Xiaojie , AI Daoying , ZHOU Hengping , ZHOU Jinsong , ZHENG Yirou , ZHOU Kai . Screening, identification, and toxin-producing ability analysis of aflatoxin producing strains in brine of Xiangpai brined products[J]. Food and Fermentation Industries, 2023 , 49(8) : 170 -176 . DOI: 10.13995/j.cnki.11-1802/ts.032703

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