Food and Fermentation Industries >

2023 , Vol. 49 >Issue 8: 342 - 350

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032358

Research progress on pretreatment and detection techniques of fumonisins in food

  • HE Lu ,
  • PAN Leiming ,
  • ZHENG Jing ,
  • XU Shuangyang ,
  • LI Yuanfei
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  • 1(Zhejiang Hongzheng Testing Co.Ltd., Ningbo 315100, China)
    2(Zanyu Technology Group Co.Ltd., Hangzhou 310009, China)

Received date: 2022-05-19

  Revised date: 2022-06-16

  Online published: 2023-05-16

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Abstract

Fumonisins (FBs) are a class of water-soluble secondary metabolites produced by Fusarium species such as Fusarium oxysporum and Fusarium verticillioides, with stable structure and properties, which have neurotoxicity, hepatotoxicity, nephrotoxicity, carcinogenicity, and other toxic side effects. As a major food production and consumption country, FBs widely exists in corn, rice, and other food crops and their products in China, the pollution rate and level of FBs are high, and the maximum value even far beyond the European maximum limit of FBs, seriously affecting the development of Chinese agricultural economy and food quality and safety. This paper summarized the pretreatment and detection techniques of FBs that had been widely used in recent years. The pretreatment techniques included immunoaffinity column, solid phase extraction, magnetic solid phase extraction, molecular imprinting technology, and QuEChERS, and the detection techniques included high-performance liquid chromatography, liquid chromatography-tandem mass spectrometry, enzyme-linked immunosorbent assay, immunochromatography assay, and biosensor method. The study focused on summarizing the principles, advantages, and limitations of different techniques, and provided an outlook on the research directions of pretreatment and detection techniques of FBs, proposing novel pretreatment materials and high-throughput rapid detection techniques, which provided support for the detection of FBs.

Key words： fumonisins; pretreatment; detection technique

Cite this article

HE Lu , PAN Leiming , ZHENG Jing , XU Shuangyang , LI Yuanfei . Research progress on pretreatment and detection techniques of fumonisins in food[J]. Food and Fermentation Industries, 2023 , 49(8) : 342 -350 . DOI: 10.13995/j.cnki.11-1802/ts.032358

References

[1] 坚乃丹, 常晓娇, 孙晶, 等.伏马毒素的危害及防控技术研究进展[J].食品工业科技, 2018, 39(7):335-339:347.
JIAN N D, CHANG X J, SUN J, et al.Research advances on the hazards and control of fumonisins[J].Science and Technology of Food Industry, 2018, 39(7):335-339;347.
[2] VOSS K A, RILEY R T, GARDNER N M, et al.Reproductive and Developmental Toxicology[M].Amsterdam:Elsevier, 2017:925-943.
[3] 黎晓雯, 罗俊崇, 张梦丹, 等.伏马毒素B1的毒性和检测方法[J].动物医学进展, 2019, 40(6):112-115.
LI X W, LUO J C, ZHANG M D, et al.Toxicity and detection methods of fumonisin B1[J].Progress in Veterinary Medicine, 2019, 40(6):112-115.
[4] 赫丹, 徐剑宏, 仇剑波, 等.伏马毒素的理化性质、检测方法及在我国玉米和玉米制品中的污染现状综述[J].江苏农业科学, 2021, 49(21):33-39.
HE D, XU J H, QIU J B, et al.Physical and chemical properties, detection methods and pollution status of fumonisins in corn and its products in China:A review[J].Jiangsu Agricultural Sciences, 2021, 49(21):33-39.
[5] CHEN J, WEN J, TANG Y T, et al.Research progress on fumonisin B1 contamination and toxicity:A review[J].Molecules (Basel, Switzerland), 2021, 26(17):5 238.
[6] 郭志青, 张霞, 刁立功, 等.镰刀菌及其伏马毒素的危害和防控[J].山东农业科学, 2022, 54(1):157-164.
GUO Z Q, ZHANG X, DIAO L G, et al.Hazards and control of Fusarium spp.and its metabolites fumonisins[J].Shandong Agricultural Sciences, 2022, 54(1):157-164.
[7] IARC. Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene[M]. Lyon: IARC Press, 2002: 64-78.
[8] 袁艺, 陆廷瑾, 沈腾腾, 等.免疫亲和柱的制备及在真菌毒素检测中应用的研究进展[J].食品工业科技, 2013, 34(24):396-400.
YUAN Y, LU T J, SHEN T T, et al.Preparation of immunoaffinity column and advances in application of mycotoxin analysis[J].Science and Technology of Food Industry, 2013, 34(24):396-400.
[9] 施雅. UPLC-MS/MS测定乳制品中伏马毒素B1、B2残留[J].食品工业, 2019, 40(11):312-315.
SHI Y.Determination of fumonisin B1, B2 in dairy products by UPLC-MS/MS[J].The Food Industry, 2019, 40(11):312-315.
[10] 张太, 毛丹, 王少敏, 等.免疫亲和柱净化-柱前衍生-高效液相色谱法测定中药薏苡仁中的伏马毒素[J].分析科学学报, 2022, 38(2):233-236.
ZHANG T, MAO D, WANG S M, et al.Determination of fumonisins in Coix seed by HPLC coupled with immunoaffinity column purification-precolumn derivatization[J].Journal of Analytical Science, 2022, 38(2):233-236.
[11] 陈悦铭, 黄景初, 徐婷, 等.多功能免疫亲和柱净化-超高效液相色谱串联质谱法快速检测咖啡豆中的11种真菌毒素[J].食品工业科技, 2021, 42(16):285-293.
CHEN Y M, HUANG J C, XU T, et al.Rapid determination of 11 mycotoxins in coffee bean through multifunctional purification and immunoaffinity column coupled to high-performance liquid chromatography tandem mass spectrometry[J].Science and Technology of Food Industry, 2021, 42(16):285-293.
[12] 田苗. 免疫亲和柱、固相萃取-高效液相色谱法测定粮谷饲料中伏马毒素B1、B2的研究[C]. 长沙:第十七届全国色谱学术报告会及仪器展览会会议论文集,2009.
TIAN M. Determination of fumonisins B1 and B2 in cereals and feeds by high performance liquid chromatography with immunoaffinity column and solid phase extraction cleanup[C]. Changsha:Proceedings of the 17th National Chromatography Symposium and Instrument Exhibition,2009.
[13] 薛秋艳, 徐剑宏, 祭芳, 等.玉米中伏马毒素FB1液相色谱-电喷雾串联质谱测定方法的建立[J].江苏农业学报, 2010, 26(3):627-630.
XUE Q Y, XU J H, JI F, et al.Establishment of high performance liquid chromatography-electrospray ionization tandem mass spectrometry for detecting fumonisin B1 in maize[J].Jiangsu Journal of Agricultural Sciences, 2010, 26(3):627-630.
[14] 林维宣, 裴轶君, 董伟峰.固相萃取-高效液相色谱法测定玉米中伏马毒素B1[J].大连轻工业学院学报, 2004, 23(1):15-17.
LIN W X, PEI Y J, DONG W F.Determination of fumonisins B1 in corn by solid phase extraction-high performance liquid chromatography[J].Journal of Dalian Institute of Light Industry, 2004, 23(1):15-17.
[15] 孙晓冬, 郝杰, 毛婷, 等.固相萃取柱净化-超高效液相色谱-串联质谱法快速测定液态乳中14种真菌毒素[J].食品科学, 2018, 39(18):292-301.
SUN X D, HAO J, MAO T, et al.Determination of 14 mycotoxins in liquid milk by solid-phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry[J].Food Science, 2018, 39(18):292-301.
[16] 张宇, 包小兵, 王冬梅, 等.超高效液相色谱串联质谱法同时测定小麦及其制品中玉米赤霉烯酮和伏马毒素的方法优化[J].河南预防医学杂志, 2021, 32(8):612-615.
ZHANG Y, BAO X B, WANG D M, et al.Optimization of the method for simultaneous determination of Zearalenone and fumonisins in wheat and its products by ultra high performance liquid chromatography-tandem mass spectrometry[J].Henan Journal of Preventive Medicine, 2021, 32(8):612-615.
[17] 马皎洁, 胡骁, 邵兵, 等.超高效液相色谱-串联质谱法测定面粉、玉米及其制品中的伏马菌素研究[J].山东大学学报(医学版), 2012, 50(4):115-120.
MA J J, HU X, SHAO B, et al.Ultra performance liquid chromatography-tandem mass spectrometry method for determination of fumonisins in wheat flour, corn and corn-based products[J].Journal of Shandong University (Health Sciences), 2012, 50(4):115-120.
[18] YU X, ZHONG T, ZHANG Y J, et al.Design, preparation, and application of magnetic nanoparticles for food safety analysis:A review of recent advances[J].Journal of Agricultural and Food Chemistry, 2022, 70(1):46-62.
[19] ZHAO Y, WAN L H, BAI X L, et al.Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction[J].Food Additives & Contaminants.Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2017, 34(7):1 201-1 210.
[20] WANG W H, ZHANG Q, MA F, et al.Simultaneous determination of aflatoxins, fumonisin B1, T-2 and cyclopiazonic acid in agri-products by immunomagnetic solid-phase extraction coupled with UHPLC-MS/MS[J].Food Chemistry, 2022, 378:132020.
[21] 郝铖, 王思齐, 李腾飞, 等.分子印迹技术在黄曲霉毒素检测中的最新研究进展[J].分析试验室, 2022, 41(3):350-356.
HAO C, WANG S Q, LI T F, et al.Recent research advances in aflatoxin detection by molecular imprinting technique[J].Chinese Journal of Analysis Laboratory, 2022, 41(3):350-356.
[22] MAO L B, JI K L, YAO L L, et al.Molecularly imprinted photoelectrochemical sensor for fumonisin B1 based on GO-CdS heterojunction[J].Biosensors and Bioelectronics, 2019, 127:57-63
[23] MUNAWAR H, SAFARYAN A H M, DE GIROLAMO A, et al.Determination of fumonisin B1 in maize using molecularly imprinted polymer nanoparticles-based assay[J].Food Chemistry, 2019, 298:125044.
[24] SINGH A K, LAKSHMI G B V S, FERNANDES M, et al.A simple detection platform based on molecularly imprinted polymer for AFB1 and FuB1 mycotoxins[J].Microchemical Journal, 2021, 171:106730.
[25] RAMALHO R R F, PEREIRA I, DA S LIMA G, et al.Fumonisin B1 analysis in maize by molecularly imprinted polymer paper spray ionization mass spectrometry(MIP-PSI-MS)[J].Journal of Food Composition and Analysis, 2022, 107:104362.
[26] 李俊超, 秦学磊, 吴圣江, 等.QuEChERS前处理方法在食品检测中的应用进展[J].食品研究与开发, 2021, 42(24):206-212.
LI J C, QIN X L, WU S J, et al.Progress in the applications of the QuEChERS method in the food determination process[J].Food Research and Development, 2021, 42(24):206-212.
[27] 李莉, 李硕.QuEChERS-超高效液相色谱-串联质谱法测定玉米油中伏马毒素B1、B2、B3[J].食品安全质量检测学报, 2020, 11(19):7 006-7 011.
LI L, LI S.Determination of fumonisins B1, B2 and B3 in corn oil by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality, 2020, 11(19):7 006-7 011.
[28] 吴基任, 潘望, 谭高好, 等.QuEChERS-超高效液相色谱-串联质谱法测定花生及土榨花生油中9种真菌毒素[J].食品安全质量检测学报, 2021, 12(10):3 927-3 935.
WU J R, PAN W, TAN G H, et al.Determination of 9 kinds of mycotoxins in peanuts and flavor peanut oil by QuEChERS-ultra performance liquid chromatographytandem mass spectrometry[J].Journal of Food Safety & Quality, 2021, 12(10):3 927-3 935.
[29] LAGO L O, NIEVIEROWSKI T H, MALLMANN L P, et al.QuEChERS-LC-QTOFMS for the simultaneous determination of legislated and emerging mycotoxins in malted barley and beer using matrix-matched calibration as a solution to the commercial unavailability of internal standards for some mycotoxins[J].Food Chemistry, 2021, 345:128744.
[30] 徐子婷, 郝莉花, 马静, 等.QuEChERS-SPE-超高效液相色谱-串联质谱法测定调味面制品中的12种真菌毒素[J].食品工业科技, 2022, 43(6):311-319.
XU Z T, HAO L H, MA J, et al.Determination of 12 mycotoxins in seasoning flour products by QuEChERS-SPE-ultra performance liquid chromatography-tandem mass spectrometry[J].Science and Technology of Food Industry, 2022, 43(6):311-319.
[31] 王军淋, 胡玲玲, 蔡增轩, 等.超高压液相色谱法同时检测玉米中的伏马毒素B1、B2、B3[J].食品安全质量检测学报, 2013, 4(1):215-223.
WANG J L, HU L L, CAI Z X, et al.Simultaneous determination of fumonisin B1, B2 and B3 in maize by ultra pressure liquid chromatography[J].Journal of Food Safety & Quality, 2013, 4(1):215-223.
[32] 陈同强, 郭锦材, 李灿, 等.柱前衍生-高效液相色谱法测定婴幼儿配方乳粉中3种伏马毒素[J].乳业科学与技术, 2021, 44(6):11-14.
CHEN T Q, GUO J C, LI C, et al.Determination of three fumonisins in infant formula by high performance liquid chromatography with precolumn derivatization[J].Journal of Dairy Science and Technology, 2021, 44(6):11-14.
[33] 郑嘉, 王红旗, 刘继红, 等.轻简化液质联用法检测玉米中的伏马毒素B1、B2和B3[J].河南农业科学, 2021, 50(9):172-180.
ZHENG J, WANG H Q, LIU J H, et al.Simplified liquid-mass coupling method for detection of fumonisins B1, B2 and B3 in corn[J].Journal of Henan Agricultural Sciences, 2021, 50(9):172-180
[34] 胡琳, 师真, 赵丽, 等.液相色谱-串联质谱法同时测定普洱茶中16种真菌毒素[J].浙江农业学报, 2019, 31(10):1 700-1 708.
HU L, SHI Z, ZHAO L, et al.Simultaneous detection and analysis of 16 kinds of mycotoxins in Pu-erh tea[J].Acta Agriculturae Zhejiangensis, 2019, 31(10):1 700-1 708.
[35] 王蕾, 张莉蕴, 王玉可, 等.快速检测技术在食品真菌毒素检测中的研究进展[J].食品研究与开发, 2021, 42(4):187-192.
WANG L, ZHANG L Y, WANG Y K, et al.Research progress of rapid detection technology in the detection of mycotoxins in food[J].Food Research and Development, 2021, 42(4):187-192.
[36] 权英, 詹月华, 张根华, 等.伏马毒素B1酶标试纸条检测方法[J].食品研究与开发, 2011, 32(6):97-100.
QUAN Y, ZHAN Y H, ZHANG G H, et al.Development of a dipstick immunoassay for rapid detecting fumonisin B1 in food samples[J].Food Research and Development, 2011, 32(6):97-100.
[37] CHEN X R, LIANG Y, ZHANG W J, et al.A colorimetric immunoassay based on glucose oxidase-induced AuNP aggregation for the detection of fumonisin B1[J].Talanta, 2018, 186:29-35.
[38] 管笛, 潘灿平, 王文, 等.磁微粒酶联免疫吸附法测定玉米中的伏马毒素B1[J].食品科学, 2014, 35(8):208-211.
GUAN D, PAN C P, WANG W, et al.Development of a magnetic particle-based enzyme-linked immunosorbent assay for determining fumonisin B1 in corn[J].Food Science, 2014, 35(8):208-211.
[39] 于瑶, 李岩松, 卢士英, 等.玉米中伏马毒素B1、B2间接竞争酶联免疫吸附方法的建立[J].食品与发酵工业, 2020, 46(23):193-197.
YU Y, LI Y S, LU S Y, et al.An indirect competitive ELISA method for detecting fumonisin B1 and B2 in maize[J].Food and Fermentation Industries, 2020, 46(23):193-197.
[40] 李向梅, 刘志威, 陈晓敏, 等.食品安全免疫层析检测技术研究进展[J].食品安全质量检测学报, 2020, 11(15):4 939-4 955.
LI X M, LIU Z W, CHEN X M, et al.Advances of immunochromatography assay for food safety[J].Journal of Food Safety & Quality, 2020, 11(15):4 939-4 955.
[41] 刘畅, 杨琳燕, 王艺霞, 等.免疫层析技术研究及应用[J].动物医学进展, 2021, 42(2):117-121.
LIU C, YANG L Y, WANG Y X, et al.Research and application of immunochromatographic analysis[J].Progress in Veterinary Medicine, 2021, 42(2):117-121.
[42] 董旭旭, 孙威, 曹攀, 等.胶体金免疫层析试纸条技术在病毒检测领域的应用研究现状[J].生物工程学报, 2022, 38(9):3 243-3 254.
DONG X X, SUN W, CAO P, et al.Colloidal gold immunochromatographic test strip for virus detection:A review[J].Chinese Journal of Biotechnology, 2022, 38(9):3 243-3 254.
[43] YAO J J, SUN Y N, LI Q M, et al.Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize[J].Journal of the Science of Food and Agriculture, 2017, 97(7):2 223-2 229.
[44] GUO L L, WANG Z X, XU X X, et al.Europium nanosphere-based fluorescence strip sensor for ultrasensitive and quantitative determination of fumonisin B1[J].Analytical Methods:Advancing Methods and Applications, 2020, 12(43):5 229-5 235.
[45] 胡高爽, 吴天琪, 苏丹, 等.基于新型标记材料的免疫分析技术在真菌毒素检测中应用的研究进展[J].食品工业科技, 2021, 42(12):398-404.
HU G S, WU T Q, SU D, et al.Research progress on application of new labeling materials based immunoassay on the detection of mycotoxin[J].Science and Technology of Food Industry, 2021, 42(12):398-404.
[46] HOU S L, MA J J, CHENG Y Q, et al.Quantum dot nanobead-based fluorescent immunochromatographic assay for simultaneous quantitative detection of fumonisin B1, dexyonivalenol, and Zearalenone in grains[J].Food Control, 2020, 117:107331.
[47] 关桦楠, 龚德状, 韩博林, 等.上转换纳米粒子在食品分析中的应用研究进展[J].食品工业, 2019, 40(3):207-211.
GUAN H N, GONG D Z, HAN B L, et al.Research progress in application of up-conversion nanoparticles in food analysis[J].The Food Industry, 2019, 40(3):207-211.
[48] LI J Z, ZHAO X D, WANG Y, et al.A highly sensitive immunofluorescence sensor based on bicolor upconversion and magnetic separation for simultaneous detection of fumonisin B1 and Zearalenone[J].The Analyst, 2021, 146(10):3 328-3 335.
[49] AZIM M A U, HASAN M, ANSARI I H, et al.Chemiluminescence immunoassay:Basic mechanism and applications[J].Bangladesh Journal of Nuclear Medicine, 2018, 18(2):171-178.
[50] XIAO Q, XU C X.Research progress on chemiluminescence immunoassay combined with novel technologies[J].TrAC Trends in Analytical Chemistry, 2020, 124:115780.
[51] 徐杰. 基于水凝胶光子晶体微球低背景化学发光技术在农产品真菌毒素检测中的应用研究[D].南京:南京师范大学, 2015.
XU J.Low background chemiluminescence detection for mycotoxins in agriculture productions based on hydrogel photonic beads[D].Nanjing:Nanjing Normal University, 2015.
[52] JIE M S, YU S C, YU F, et al.An ultrasensitive chemiluminescence immunoassay for fumonisin B1 detection in cereals based on gold-coated magnetic nanoparticles[J].Journal of the Science of Food and Agriculture, 2018, 98(9):3 384-3 390.
[53] 窦博鑫, 张云亮, 王艳, 等.生物传感器在食品检测领域的应用研究进展[J].食品安全质量检测学报, 2022, 13(3):845-851.
DOU B X, ZHANG Y L, WANG Y, et al.Advances in the application of biosensors in the field of food detection[J].Journal of Food Safety & Quality, 2022, 13(3):845-851.
[54] 钱佳婕, 黄迪, 徐颖华, 等.食源性致病微生物检测技术研究进展[J].食品安全质量检测学报, 2021, 12(12):4 775-4 785.
QIAN J J, HUANG D, XU Y H, et al.Research progress of detection technologies for foodborne pathogens[J].Journal of Food Safety & Quality, 2021, 12(12):4 775-4 785.
[55] LI M, LI D Y, LI Z Y, et al.A visual peroxidase mimicking aptasensor based on Pt nanoparticles-loaded on iron metal organic gel for fumonisin B1 analysis in corn meal[J].Biosensors and Bioelectronics, 2022, 209:114241.
[56] YU Y, LI Y S, ZHANG Q, et al.Colorimetric immunoassay via smartphone based on Mn2+-Mediated aggregation of AuNPs for convenient detection of fumonisin B1[J].Food Control, 2022, 132:108481.
[57] 王琦, 杨庆利, 吴薇.基于氧化石墨烯的荧光适配体传感器检测食品中真菌毒素[J].食品科学, 2021, 42(24):318-322.
WANG Q, YANG Q L, WU W.A graphene oxide-based fluorescent aptasensor for determination of mycotoxins in foods[J].Food Science, 2021, 42(24):318-322.
[58] QIN Y K, LI S, WANG Y, et al.A highly sensitive fluorometric biosensor for fumonisin B1 detection based on upconversion nanoparticles-graphene oxide and catalytic hairpin assembly[J].Analytica Chimica Acta, 2022, 1207:339811.
[59] DONG N, LIU D, MENG S Y, et al.Tetrahedral DNA nanostructure-enabled electrochemical aptasensor for ultrasensitive detection of fumonisin B1 with extended dynamic range[J].Sensors and Actuators B:Chemical, 2022, 354:130984.
[60] MUNAWAR H, GARCIA-CRUZ A, MAJEWSKA M, et al.Electrochemical determination of fumonisin B1 using a chemosensor with a recognition unit comprising molecularly imprinted polymer nanoparticles[J].Sensors and Actuators B:Chemical, 2020, 321:128552.
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