Food and Fermentation Industries >

2023 , Vol. 49 >Issue 9: 164 - 170

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032847

High sensitive detection of dichlorvos by acetylcholinesterase sensor based on carbon aerogel@gold nanomaterials/ionic liquid

  • LI Xiaoqian ,
  • SHAO Zheng
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  • 1(Puyang Vocational and Technical College, Puyang 457000, China)
    2(Changyuan Cooking Vocational and Technical College, Xinxiang 453400, China)

Received date: 2022-07-02

  Revised date: 2022-08-26

  Online published: 2023-06-05

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Abstract

Nitrogen doped carbon aerogel(N-CAs) was prepared based on carbon aerogels and gold nanorods (Au) was loaded on N-CAs for a new nitrogen doped carbon aerogel@gold nanorods (N-CA@Au). Acetylcholinesterase chloride (AChE) sensor AChE/N-CA@Au-[BSMIM]HSO4/BDD was prepared for detecting dichlorvos in vegetables using boron doped diamond film electrode (BDD) as working electrode, N-CA@Au and 1-(4-sulfonic acid) butyl-3-methylimidazole bisulfate ionic liquid ([BSMIM]HSO4) as modified materials. The study found that N-CA@Au had a good conductivity and adsorption characteristics, [BSMIM]HSO4 could provide a stable electrochemical reaction environment for AChE/N-CA@Au-[BSMIM]HSO4/BDD and make AChE, N-CA@Au and BDD play a good synergy, which can effectively improve the sensitivity of the sensor. Under the optimum test conditions, there was a linear relationship between the concentration of dichlorvos and the negative logarithm of the inhibition rate of AChE/N-CA@Au-[BSMIM]HSO4/BDD, and the detection limit was 1.967 4×10-11 g/L (calculated according to the inhibition rate of 10%), the linear range was wide, detection limit was low, stability and reproducibility was good, and the sensor had a good anti-interference ability to common heavy metal ions. AChE/N-CA@Au-[BSMIM]HSO4/BDD was simple, convenient and fast, can be used for rapid quantitative analysis of dichlorvos.

Key words： dichlorvos; enzyme inhibition; carbon aerogel; ionic liquids; gold nanomaterials; sensors

Cite this article

LI Xiaoqian , SHAO Zheng . High sensitive detection of dichlorvos by acetylcholinesterase sensor based on carbon aerogel@gold nanomaterials/ionic liquid[J]. Food and Fermentation Industries, 2023 , 49(9) : 164 -170 . DOI: 10.13995/j.cnki.11-1802/ts.032847

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