A qualitative and quantitative method for formaldehyde in alcohol was established based on ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS). Formaldehyde in the sample reacts with 2,4-dinitrophenylhydrazine under acidic conditions to form a stable derivative formaldehyde-2,4-dinitrophenylhydrazone. The product was analyzed by UPLC-MS/MS and external standard method was used for quantification. The results showed that formaldehyde had a good linear relationship in 0.01-0.2 mg/L, the correlation coefficient was more than 0.999, the detection limit was 0.002 mg/L, and the recovery rate of standard addition in beer, liquor, wine and rice wine could reach 93.7%-97.5%, the relative standard deviation (RSD) of the precision test results was 2.71% to 4.98%. The presented method is easy to operate with high sensitivity, and can accurately and rapid quantitative analysis of formaldehyde in different wine samples, which provides a reference for the detection of formaldehyde content in wine.
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