Food and Fermentation Industries >

2023 , Vol. 49 >Issue 9: 304 - 309

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032640

Rapid determination of 11 biotoxins in maize and its products by ultra-performance liquid chromatography-mass spectrometry

  • LI Caihong ,
  • WANG Yonghong ,
  • ZHAO Rulang ,
  • ZHANG Weijun ,
  • ZHAO Jian ,
  • LIU Xia ,
  • ZHAO Zidan
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  • 1(Institute of Quality Standard and Testing Technology for Agro-Products of Ningxia, Yinchuan 750002, China)
    2(The Crop Research Institute, Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China)
    3(Key Laboratory of Agricultural Product Quality Standard Research and Evaluation of Ningxia Hui Autonomous Region, Yinchuan 750002, China)

Received date: 2022-06-11

  Revised date: 2022-07-16

  Online published: 2023-06-05

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Abstract

To establish a rapid method for determining 11 mycotoxins in corn kernels using ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry. Samples were extracted with 80% acetonitrile, separated on an HSS T3 chromatographic column (2.1 mm×100 mm, 1.8 μm), and gradient eluted with 0.1% methanol containing formic acid-5 mmol/L ammonium acetate using 0.1% formic acid in water as mobile phase. Electrospray ionization (ESI), multiple reaction monitoring (MRM) in positive and negative ion modes and the external standard method were used for mass spectrometry. The 11 mycotoxins showed a good linear relationship in a certain concentration range, with the correlation coefficient r>0.999 5, and the limit of quantitation<0.001 mg/kg. At the addition level of 0.001-2.0 mg/kg, each addition level was repeated for 5 times, revealing that the mean recovery was 72%-88% and the relative standard deviation was 0.8%-13.4%. This method is accurate, rapid and efficient for quantifying the 11 mycotoxins in corn kernels, with high practicability.

Key words： maize; maize products; ultra performance liquid chromatography-mass spectrometry/mass spectrometry; biotoxin; rapid determination

Cite this article

LI Caihong , WANG Yonghong , ZHAO Rulang , ZHANG Weijun , ZHAO Jian , LIU Xia , ZHAO Zidan . Rapid determination of 11 biotoxins in maize and its products by ultra-performance liquid chromatography-mass spectrometry[J]. Food and Fermentation Industries, 2023 , 49(9) : 304 -309 . DOI: 10.13995/j.cnki.11-1802/ts.032640

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