Antrodia cinnamomea is a precious and popular edible and medicinal mushroom. Submerged fermentation based on spore inoculation is considered the most efficient and popular artificial cultivation method to produce A. cinnamomea. Improving the yield and speed of sporulation is beneficial to improve the inoculum preparation efficiency and industrial production benefit of A. cinnamomea in submerged fermentation. Thus, the metal ions that can significantly promote sporulation of A. cinnamomea were selected by single factor method, then the response surface methodology was used to optimize the concentrations of the metal ion combination. It was found that separately adding 1.0, 0.1, 0.1 mmol/L of Ca2+, Fe2+, or Zn2+ to the medium markedly increased the sporulation of A. cinnamomea by 87.21%, 72.39%, and 35.05%, respectively. Adding 0.149, 2.02, 0.135 mmol/L of Fe2+, Ca2+, and Zn2+ together, resulted in an 8.67×107 spores/mL sporulation of A. cinnamomea, 133.62% higher compared to that of the control. Meanwhile, the time for sporulation of A. cinnamomea reaching the maximum was shortened by 24 h. The present study is easy to operate, low-cost, and effective, thereby is of great value in development and application.
LI Huaxiang
,
WANG Juanjuan
,
SHI Yu
,
JI Dan
,
GAO Yajun
,
JIA Luqiang
,
RAO Shengqi
,
YANG Zhenquan
. Metal ions promoting sporulation of Antrodia cinnamomea in submerged fermentation[J]. Food and Fermentation Industries, 2023
, 49(10)
: 55
-63
.
DOI: 10.13995/j.cnki.11-1802/ts.033127
[1] 谢春芹, 凡军民, 宋振潇, 等.牛樟芝液体发酵培养最适营养条件优化[J].北方园艺, 2019(19):106-113.
XIE C Q, FAN J M, SONG Z X, et al.Optimization of optimal nutritional conditions for liquid fermentation culture of Antrodia camphorate[J].Northern Horticulture, 2019(19):106-113.
[2] LU C L, LI H X, ZHU X Y, et al.Regulatory effect of intracellular polysaccharides from Antrodia cinnamomea on the intestinal microbiota of mice with antibiotic-associated diarrhea[J].Quality Assurance and Safety of Crops & Foods, 2022, 14(3):124-134.
[3] 黄兴连. 浅谈牛樟芝研究现状[J].南方农业, 2021, 15(21):27-28.
HUANG X L.A brief talk on the present research situation of Antrodia[J].South China Agriculture, 2021, 15(21):27-28.
[4] 李华祥, 石瑀, 罗志珊, 等. 牛樟树提取物促进樟芝深层发酵无性产孢及其应用[J]. 食品与发酵工业, 2022, 48(16):65-71.
LI H X, SHI Y, LUO Z S, et al. Cinnamomum camphora extract promotes asexual sporulation of Antrodia camphorata by submerged fermentation and its application[J]. Food and Fermentation Industries, 2022, 48(16):65-71.
[5] LU Z M, LEI J Y, XU H Y, et al.Optimization of fermentation medium for triterpenoid production from Antrodia camphorata ATCC 200183 using artificial intelligence-based techniques[J].Applied Microbiology and Biotechnology, 2011, 92(2):371-379.
[6] LI H X, LU Z M, GENG Y, et al.Efficient production of bioactive metabolites from Antrodia camphorata ATCC 200183 by asexual reproduction-based repeated batch fermentation[J].Bioresource Technology, 2015, 194:334-343.
[7] GANESAN N, BASKARAN R, VELMURUGAN B K, et al.Antrodia cinnamomea—An updated minireview of its bioactive components and biological activity[J].Journal of Food Biochemistry, 2019, 43(8):e12936.
[8] LI H X, LU Z M, ZHU Q, et al.Comparative transcriptomic and proteomic analyses reveal a flug-mediated signaling pathway relating to asexual sporulation of Antrodia camphorata[J].Proteomics 2017, 17:1700256.
[9] HSIAO H Y, WENG Y M.Hepatoprotective effect of an Antrodia cinnamomea product via a novel process on carbon tetrachloride-induced hepatotoxicity in rats[J].International Journal of Nutrition and Food Sciences, 2018, 7(4):134.
[10] 李华祥, 陆震鸣, 朱青, 等.钙离子调控樟芝深层发酵无性产孢及其分子机制[J].生物工程学报, 2017, 33(7):1 124-1 135.
LI H X, LU Z M, ZHU Q, et al.Effect of calcium on sporulation of Taiwanofungus camphoratus in submerged fermentation[J].Chinese Journal of Biotechnology, 2017, 33(7):1 124-1 135.
[11] 李华祥, 陆震鸣, 耿燕, 等.樟芝深层发酵工艺研究进展[J].菌物学报, 2017, 36(10):1 332-1 345.
LI H X, LU Z M, GENG Y, et al.Recent advance in submerged fermentation of Taiwanofungus camphoratus[J].Mycosystema, 2017, 36(10):1 332-1 345.
[12] LIU X F, XIA Y J, LAI P F H, et al.An increase in cell membrane permeability in the in situ extractive fermentation improves the production of antroquinonol from Antrodia camphorata S-29[J].Journal of Industrial Microbiology and Biotechnology, 2020, 47(2):197-207.
[13] XIA Y J, CHEN Y, LIU X F, et al.Enhancement of antroquinonol production during batch fermentation using pH control coupled with an oxygen vector[J].Journal of the Science of Food and Agriculture, 2019, 99(1):449-456.
[14] ZHANG Y T, LI D Y, WANG Z, et al.Alpha-terpineol affects synthesis and antitumor activity of triterpenoids from Antrodia cinnamomea mycelia in solid-state culture[J].Food & Function, 2018, 9(12):6 518-6 526.
[15] LU Z M, HE Z, LI H X, et al.Modified arthroconidial inoculation method for the efficient fermentation of Antrodia camphorata ATCC 200183[J].Biochemical Engineering Journal, 2014, 87:41-49.
[16] 李华祥. 樟芝无性孢子介导的高效循环发酵策略及其产孢分子机制解析[D].无锡:江南大学, 2017.
LI H X.Arthroconidia-mediated repeated batch fermentation strategy for Antrodia camphorate and underlying mechanism of asexual sporulation[D].Wuxi:Jiangnan University, 2017.
[17] 朱炎平, 邱君志, 谢雪钦, 等.碳源、氮源、金属离子和维生素对虫生真菌粉虱座壳孢孢子萌发、菌丝生长和产孢的影响[C].中国第六届海峡两岸菌物学学术研讨会.北京:中国菌物学会, 2004:123-127.
ZHU J P, QIU J Z, XIE X Q, et al.Effects of carbon sources, nitrogen sources, metal ions and vitamins on spore germination, hyphal growth and sporulation of entomopathogenic fungus Aschersonia aleyrodis Webber[C].Proceedings of the Sixth Cross-strait Symposium on Bacteriology in China.Beijing:Mycological Society of China, 2004:123-127.
[18] 刘雨佳. 玉米小斑病菌(Cochliobolus heterostrophus)转录因子ChhapX基因功能初步研究[D].沈阳:沈阳农业大学, 2020.
LIU Y J.Functional study on transcription factor ChhapX of Cochliobolus heterostrophus[D].Shenyang:Shenyang Agricultural University, 2020.
[19] 李芳, 张珏锋, 钟海英, 等.锌离子响应转录激活因子ZafA对球孢白僵菌锌离子利用及生防潜能的影响[J].菌物学报, 2022, 41(4):570-586.
LI F, ZHANG J F, ZHONG H Y, et al.Effects of zinc-responsive transcriptional activator ZafA on zinc utilization and biocontrol potential of Beauveria bassiana[J].Mycosystema, 2022, 41(4):570-586.
[20] 王震. 扁座壳孢菌生理特性及乳悬剂的初步研究[D].福州:福建农林大学, 2009.
WANG Z.Preliminary study on physiological characteristics and emulstfiable suspension of Aschersonia placenta[D].Fuzhou:Fujian Agriculture and Forestry University, 2009.
[21] ČÍK G, BUJDÁKOVA H, ŠERŠEŇ F.Study of fungicidal and antibacterial effect of the Cu (II)-complexes of thiophene oligomers synthesized in ZSM-5 zeolite channels[J].Chemosphere, 2001, 44(3):313-319.
[22] LU Z M, GENG Y, LI H X, et al.Alpha-terpineol promotes triterpenoid production of Antrodia cinnamomea in submerged culture[J].FEMS Microbiology Letters, 2014, 358(1):36-43.
