There are rich varieties of trace components in Xifeng liquor, but there is little research on the effects and dose-effect relationships of these trace components. Therefore, the effects of trace components in Xifeng liquor (represented by Hongxifeng) on LO2 cells and intestinal flora of healthy mice were investigated based on cell and animal experiments. The results of cell experiments showed that the higher ethanol concentration, the greater the cell inhibition rate, ALT and AST leakage. At the same ethanol concentration, there was a significant difference between Hongxifeng and edible alcohol (control group) intervention groups (P<0.05). The results of animal experiment showed that intervention with different doses of Hongxifeng and edible alcohol could reduce body weight and HDL-C of mice, increase liver weight ratio, AST, ALT, TC, TG, and LDL-C, induce different degrees of steatosis in liver tissues, and significantly change intestinal flora (at the phylum level, the ratio of Firmicutes to Bacteroides decreased, while at the genus level, the relative abundance of Lactobacillus and Lachnospira decreased, and the relative abundance of Helicobacter and Streptococcus increased). LEfSe analysis results showed that the difference in intestinal flora of mice was related to the intervention dose of Hongxifeng and edible alcohol, and the effect of high-dose intervention on intestinal flora of mice tended to be expanded. However, at the same dose, the damage degree of Hongxifeng intervention on mice was lighter than that of edible alcohol, and the effect of low-dose Hongxifeng intervention on mice was the mildest, suggesting that each trace component in Hongxifeng might regulate the intestinal flora of mice to reduce the damage to the liver. The above results indicated that moderate intake of Hongxifeng could alleviate the negative effects of alcohol on LO2 cells and intestinal flora of mice to a certain extent, and this study would provide a theoretical basis for the healthy consumption of Xifeng liquor.
FU Huan
,
HU Fengrui
,
LIU Lili
,
CHEN Xuefeng
,
JIA Zhiyong
,
YAN Zongke
,
CHEN Mengyin
,
ZHOU Duan
,
ZHAO Yanni
,
CHENG Wei
. Effect of Xifeng liquor intervention on LO2 cells and intestinal flora of healthy mice[J]. Food and Fermentation Industries, 2023
, 49(10)
: 213
-222
.
DOI: 10.13995/j.cnki.11-1802/ts.032750
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