Food and Fermentation Industries >

2023 , Vol. 49 >Issue 11: 298 - 305

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033098

Research progress of nucleic acid amplification technology in animal food adulteration

  • MENG Xiaoshuai ,
  • HU Hezhi ,
  • YAN Tingting ,
  • LI Hui ,
  • LI Shanshan
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  • 1(Hebei Key Laboratory of Smart Sensing and Human-robot Interactions, Hebei University of Technology, Tianjin 300130, China)
    2(State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300132, China)
    3(Hebei University of Technology, Langfang 065000, China)

Received date: 2022-07-25

  Revised date: 2022-08-22

  Online published: 2023-06-30

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Abstract

Nucleic acid amplification technology is a molecular biology technique to rapidly amplify specific DNA fragments in vitro, which has been widely used in research fields such as infectious disease detection, biological survey, food safety detection, clinical diagnosis, and public health monitoring. Among them, food safety issues have gradually become the focus, especially the phenomenon of animal food adulteration. In the past scientific research, the application of nucleic acid amplification technology for food adulteration of animal origin has developed rapidly and made great technical progress. This article reviews the principles of gel electrophoresis PCR, quantitative real-time PCR, droplet digital PCR, loop-mediated isothermal amplification, cross prime amplification, rolling loop amplification, and recombinase polymerase amplification among nucleic acid amplification techniques, and their applications in the detection of animal food adulteration. The key advantages and limitations of various nucleic acid amplification techniques are discussed, and the existing challenges and further research progress are outlined, aiming to point the way for the development of nucleic acid amplification techniques for animal food adulteration.

Key words： nucleic acid amplification technology; animal meat food; adulteration identification; polymerase chain reaction; isothermal amplification technology

Cite this article

MENG Xiaoshuai , HU Hezhi , YAN Tingting , LI Hui , LI Shanshan . Research progress of nucleic acid amplification technology in animal food adulteration[J]. Food and Fermentation Industries, 2023 , 49(11) : 298 -305 . DOI: 10.13995/j.cnki.11-1802/ts.033098

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