Food and Fermentation Industries >

2023 , Vol. 49 >Issue 12: 85 - 91

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033092

Optimization of Volvariella volvacea liquid culture medium and its cultivation comparison with solid starter

  • TAN Qiangfei ,
  • WANG Wenpei ,
  • CHENG Zhihong ,
  • WANG Jing ,
  • WANG Qiaoli ,
  • YUN Jianmin ,
  • ZHAO Fengyun
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  • 1(College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)
    2(Special Agricultural Development Center of Kangle County, Linxia Hui Autonomous Prefecture, Gansu Province, Linxia 731100, China)

Received date: 2022-07-25

  Revised date: 2022-09-01

  Online published: 2023-07-13

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Abstract

Liquid strains were more and more used in the factory and automated production of edible fungi.In this paper, through single factor and response surface tests, medium formula of liquid starter of Volvariella volvacea was optimized, and the cultivation comparison was carried out with liquid and solid starters of V.volvacea.The results showed that the optimal carbon source, nitrogen source, and inorganic salt of V.volvacea liquid culture medium were glucose, yeast powder, and KH2PO4, respectively.The optimal formulation of the medium for liquid starter was 20.60 g/L glucose, 4.10 g/L yeast powder, and 2.30 g/L KH2PO4 into potato dextrose broth, the dry weight of V.volvacea mycelium under this formula condition was 6.45 g/L.Cultivation experiments showed that, compared with solid starter, liquid starter could shorten the production cycle of V.volvacea by about 4 days, and significantly increase the diameter of fruiting bodies and the weight of single mushroom (P<0.05), but the difference in biological efficiency between the two was not significant (P>0.05).This study can provide a reference for the development and industrial utilization of V.volvacea liquid strains.

Key words： Volvariella volvacea; liquid strain; solid strain; response surface optimization; cultivation comparison

Cite this article

TAN Qiangfei , WANG Wenpei , CHENG Zhihong , WANG Jing , WANG Qiaoli , YUN Jianmin , ZHAO Fengyun . Optimization of Volvariella volvacea liquid culture medium and its cultivation comparison with solid starter[J]. Food and Fermentation Industries, 2023 , 49(12) : 85 -91 . DOI: 10.13995/j.cnki.11-1802/ts.033092

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