Food and Fermentation Industries >

2023 , Vol. 49 >Issue 12: 194 - 201

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033135

Isolation and purification of angiotensin-converting enzyme inhibitory peptides from the digestive hydrolysate of simulated gastrointestinal fluid of the Enteromorpha clathrata

  • ZHANG Di ,
  • LUO Wei ,
  • LIANG Haidi ,
  • WANG Yuhan ,
  • GUO Tianfu
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China)
    2(Zhanjiang Preschool Education College, Zhanjiang 524084, China)
    3(College of Basic Education, Lingnan Normal University, Zhanjiang 524037, China)
    4(Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China)
    5(Marine Food Engineering and Technology Research Center of Guangdong Provence, Zhanjiang 524088, China)
    6(Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, China)

Received date: 2022-07-29

  Revised date: 2022-08-31

  Online published: 2023-07-13

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Abstract

In order to prepare angiotensin-converting enzyme (ACE) inhibitory peptides from simulated gastrointestinal digestion products of Enteromorpha clathrata and to characterize their structures, the E.clathrata was used as raw material for preparation of hydrolysis of simulated gastrointestinal digestion products.And in vitro ACE inhibition rate was used as an indicator.The amino acid sequences of the ACE inhibitory peptides were analyzed and identified by high performance liquid-quadrupole time-of-flight tandem mass spectrometry.The results showed that the ACE inhibitory active peptide in the hydrolysate of simulated gastrointestinal digestion of E.clathrata was mainly distributed in the ultrafiltration fraction with H4.A new ACE inhibitory peptide was found with molecular weight of 348.1 Da and peptide sequence of Leu-Tyr was identified after isolation and purification.And the IC50 value of ACE inhibitory peptide was (0.12±0.01) mg/mL and purified by 11.17-fold.Thus, E.clathrata is a kind of beneficial and nutritional food and its simulated gastrointestinal digestive product contains ACE inhibitory peptide, which is a pharmaceutical component for the treatment of hypertension.E.clathrata has potential as an ingredient for hypotensive health products.

Key words： Enteromorpha clathrata; simulation of gastrointestinal digestion; angiotensin-converting enzyme inhibitory peptide; purification and identification

Cite this article

ZHANG Di , LUO Wei , LIANG Haidi , WANG Yuhan , GUO Tianfu . Isolation and purification of angiotensin-converting enzyme inhibitory peptides from the digestive hydrolysate of simulated gastrointestinal fluid of the Enteromorpha clathrata[J]. Food and Fermentation Industries, 2023 , 49(12) : 194 -201 . DOI: 10.13995/j.cnki.11-1802/ts.033135

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