Food and Fermentation Industries >

2023 , Vol. 49 >Issue 12: 251 - 257

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032728

Simultaneous determination of 96 drug residues in bean sprouts by dispersive-SPE-ultra performance liquid chromatography-tandem mass spectrometry

  • MA Kai ,
  • YANG Changbiao ,
  • CUI Shanshan ,
  • YANG Chunping
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  • (Guizhou Academy of Testing and Analysis, Guiyang 550000, China)

Received date: 2022-06-20

  Revised date: 2022-08-05

  Online published: 2023-07-13

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Abstract

A modified dispersive-SPE coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of 96 kinds of plant growth regulators, antibiotics, and fungicides in bean sprouts.Samples were extracted with 1% formic acid-acetonitrile and salted out with anhydrous sodium sulfate and sodium chloride, and then the supernatant was purified with C18 as the adsorbent.The analytes were separated on a C18 chromatographic column using gradient elution with acetonitrile-5 mmol/L ammonium acetate in water containing 0.1% formic acid aqueous solution as the mobile phase, detected by MS/MS in both positive ion and negative ion mode using dynamic multiple reaction monitoring (DMRM), and finally quantified by the matrix-matched external standard method.Results showed that good linearity was observed for the 96 compounds with correlation coefficients greater than 0.990 in the corresponding mass concentration ranges. The limits of quantitation were between 5 and 50 μg/kg. The average recoveries at three spiked levels of 10, 50, and 200 μg/kg were in the range of 75.8%-117.7%, 64.3%-114.5%, and 64.3%-114.4%, with relative standard deviations (RSDs) of 2.3%-12.9%, 1.2%-14.1%, and 2.4%-17.5%.The method was applied to the determination of 72 bean sprout samples.In all, 8 drug residues were detected in the 19 samples.This method is simple, rapid, accurate, and reliable with wide coverage, which can effectively evaluate the types and content of drug residues in bean sprouts, and provide technical support for risk supervision of drug residues in bean sprouts.

Key words： bean sprouts; dispersive-SPE; ultra-high performance liquid chromatography-tandem mass spectrometry; drug residues

Cite this article

MA Kai , YANG Changbiao , CUI Shanshan , YANG Chunping . Simultaneous determination of 96 drug residues in bean sprouts by dispersive-SPE-ultra performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2023 , 49(12) : 251 -257 . DOI: 10.13995/j.cnki.11-1802/ts.032728

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