Food and Fermentation Industries >

2023 , Vol. 49 >Issue 13: 40 - 48

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033466

Screening of a marine chitinase-producing bacterium and its enzymatic properties

  • YAN Jiajia ,
  • YOU Tian ,
  • ZHANG Xuemei ,
  • LI Xinge ,
  • XUE Xianli ,
  • WANG Depei
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  • 1(College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China)
    2(Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin 300457, China)
    3(Tianjin Microbial Metabolism and Fermentation Process Control Technology Engineering Center, Tianjin 300457, China)

Received date: 2022-08-29

  Revised date: 2022-09-23

  Online published: 2023-08-07

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Abstract

Ocean is a major source of chitin in nature, which encourages a wide variety of microorganisms capable of degrading chitinase. Therefore, screening bacteria with high activity of chitinase from the ocean is an effective way to obtain microorganisms with high yield of chitinase. In this paper, a bacterial strain Y-8, which can degrade chitin, was screened from the sea crab and its habitant-Bohai mudflats, with colloidal chitin as the sole carbon source, and its identification and the enzymatic properties of its chitinase were studied. Strain Y-8 was identified as Vibrio parahaemolyticus by molecular identification. SDS-PAGE and protein spectrum analysis of the fermentation broth supernatant revealed the presence of two chitinases. Chi1 chitinase, with amino acid residue of 848, has theoretical molecular weight of 87.6 kDa. Chi2 chitin endonuclease has a residual amino acid number of 1 054 and a theoretical molecular weight of 112.9 kDa. The chitinase produced by Y-8 could effectively degrade colloidal chitin to obtain a single product N-acetyl glucosamine. The optimal reaction temperature was 55 ℃, and the enzyme retained more than 70% activity after 1 h at 45 ℃. The optimum pH was 6.0, and the enzyme retained more than 55% relative enzyme retained after 1 h at pH 4.0-9.0 37 ℃, showing good stability. 10 mmol/L Mn2+ could increase chitinase activity by 362%, while EDTA, SDS, tween-20, tween-80 could inhibit chitinase activity.

Key words： strain screening; Vibrio parahaemolyticus; chitin; chitinase; enzymatic properties

Cite this article

YAN Jiajia , YOU Tian , ZHANG Xuemei , LI Xinge , XUE Xianli , WANG Depei . Screening of a marine chitinase-producing bacterium and its enzymatic properties[J]. Food and Fermentation Industries, 2023 , 49(13) : 40 -48 . DOI: 10.13995/j.cnki.11-1802/ts.033466

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