Food and Fermentation Industries >

2023 , Vol. 49 >Issue 13: 114 - 120

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034245

Optimization of low temperature spray drying process and characteristics of Lactiplantibacillus plantarum P-8

  • LIU Qingxuan ,
  • ZHENG Xinfei ,
  • ZHANG Jingwen ,
  • ZHANG Heping ,
  • YAO Guoqiang
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  • (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China)

Received date: 2022-11-10

  Revised date: 2022-11-23

  Online published: 2023-08-07

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Abstract

The preparation of probiotic preparations by spray drying has the advantages of low cost, large processing capacity and continuous production. However, its application is limited by the high temperature damage to the activity of the bacteria. To reduce the thermal damage and improve the survival rate of the bacteria, low temperature spray drying was applied in this study. Through single factor experiment and response surface center combination design, the optimum conditions to prepare Lactiplantibacillus plantarum P-8 active bacteria powder were obtained. The morphological changes and storage stability of strain were concerned. Tolerance in a dynamic extracorporeal bionic gastrointestinal digestive model was explored. The highest survival rate of L. plantarum P-8 was 81.50% at the feed rate of 9 mL/min, drying temperature of 75 ℃ and atomization pressure of 0.3 MPa, while the form of bacteria powder particles remained complete. Good tolerance showed after continuous digestion for 4 h in a dynamic in vitro bionic gastrointestinal digestion model. The survival rates of the bacterial powder were 92.18% and 87.49% after 35 days of storage at 4 and 25 ℃, respectively. The low-temperature spray drying technology can be used for the preparation of highly active bacterial powder of L. plantarum P-8. The optimized process parameters provide a reference for the pilot preparation of L. plantarum P-8.

Key words： Lactiplantibacillus plantarum P-8; spray drying at low temperature; process optimization; dynamic biomimetic digestive system in vitro; storage activity

Cite this article

LIU Qingxuan , ZHENG Xinfei , ZHANG Jingwen , ZHANG Heping , YAO Guoqiang . Optimization of low temperature spray drying process and characteristics of Lactiplantibacillus plantarum P-8[J]. Food and Fermentation Industries, 2023 , 49(13) : 114 -120 . DOI: 10.13995/j.cnki.11-1802/ts.034245

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