This study developed a simple and efficient method that converted pineapple leaves into bioethanol, yeast protein, and fiber materials. Pineapple leaves produced leaf juice and fiber materials through mechanical pressing. Each ton of fresh pineapple leaves could produce 0.75 t leaf juice and 0.12 t dry fiber. The nutrient components of the pineapple leaf juice could completely provide the requirements for the growth of Saccharomyces cerevisiae. The sugar contained in the pineapple leaf juice was mainly glucose and fructose. Saccharomyces cerevisiae was adopted for anaerobic fermentation to produce bioethanol and yeast protein. Through the optimization of fermentation parameters, under the conditions of temperature 30 ℃, initial pH 4.0, and inoculum size of 10% (volume fraction), the maximum yield of ethanol was up to 12.88 g/L and the dry yeast powder was up to 3.83 g/L. The result of this study can provide an experimental reference for the comprehensive utilization of pineapple leaves.
WANG Liuhe
,
ZHANG Yuyu
,
GAO Chen
,
LI Song
,
CHEN A'na
. Study on process of producing biological products by high-value utilization of pineapple leaves[J]. Food and Fermentation Industries, 2023
, 49(13)
: 250
-254
.
DOI: 10.13995/j.cnki.11-1802/ts.032350
[1] SHU H Y, SUN W, XU G Y, et al.The situation and challenges of pineapple industry in China[J].Agricultural Sciences, 2019, 10(5):683-688.
[2] 刘传和, 贺涵, 何秀古, 等.我国菠萝品种结构与新品种自主选育推广[J].中国热带农业, 2021(4):13-15;76.
LIU C H, HE H, HE X G, et al.Pineapple cultivar structure and extension of new independent breeding pineapple cultivars in China[J].China Tropical Agriculture, 2021(4):13-15;76.
[3] 汪泽, 崔丽虹, 付调坤, 等.菠萝叶的化学成分及生物活性研究进展[J].化工新型材料, 2016, 44(11):258-260.
WANG Z, CUI L H, FU T K, et al.Research progress in chemical component and biological activity of pineapple leaf[J].New Chemical Materials, 2016, 44(11):258-260.
[4] 何运燕, 欧仕益.菠萝茎营养成分的测定[J].现代食品科技, 2008, 24(10):1061-1062;972.
HE Y Y, OU S Y.Determination of nutrients in pineapple peduncle[J].Modern Food Science and Technology, 2008, 24(10):1061-1062;972.
[5] SOMMA D, LOBKOWICZ H, DEASON J P.Growing America's fuel:An analysis of corn and cellulosic ethanol feasibility in the United States[J].Clean Technologies and Environmental Policy, 2010, 12(4):373-380.
[6] WEINBERG Z G, KHANAL P, YILDIZ C, et al. Effects of stage of maturity at harvest, wilting and LAB inoculant on aerobic stability of wheat silages[J]. Animal Feed Science and Technology, 2010, 158(1-2):29-35.
[7] KARP S G, MEDINA J D C, LETTI L A J, et al.Bioeconomy and biofuels:The case of sugarcane ethanol in Brazil[J].Biofuels, Bioproducts and Biorefining, 2021, 15(3):899-912.
[8] KEIL M, KLENK I, KUNZ M, et al.European bioethanol from grain and sugarbeet from an economic and ecological viewpoint (summary of Parts 1-3)[J].Zuckerindustrie Sugar Industry, 2009, 134(2):127-130.
[9] 蔡柳, 曾璐, 张婷婷, 等.薯类原料生物转化燃料乙醇研究进展[J].中国酿造, 2011, 30(3):1-5.
CAI L, ZENG L, ZHANG T T, et al.Review on fuel ethanol production from tuber crop[J].China Brewing, 2011, 30(3):1-5.
[10] 马欢, 刘伟伟, 张无敌, 等.燃料乙醇的研究进展及存在问题[J].能源工程, 2006(2):29-33.
MA H, LIU W W, ZHANG W D, et al.Present situation and existing problem of fuel alcohol[J].Energy Engineering, 2006(2):29-33.
[11] 王秀奇. 基础生物化学实验[M].2版.北京:高等教育出版社, 1999.
WANG X Q.Basic Biochemistry experiment edition[M].2nd ed.Beijing:Higher Education Press,1999.
[12] 吴丽荣, 陈肇娜, 苏丽婷, 等.DB-WAX毛细管柱气相色谱法检查藿香正气水中乙醇量[J].化学与粘合, 2020, 42(2):150-152.
WU L R, CHEN Z N, SU L T, et al.The determination of ethanol amount in huoxiangzhengqi liquid by gas chromatographic method(GC) with DB-WAX capillary column[J].Chemistry and Adhesion, 2020, 42(2):150-152.
[13] SLUITER A, HAMES B, RUIZ R, et al. Determination of Structural Carbohydrates and Lignin in Biomass Laboratory Analytical Procedure (LAP)[EB/OL](2008-04-25)[2022-05-16] https://www.nrel.gov/docs/gen/fy13/42618.pdf.
[14] 陶庭先. 工科化学实验[M].北京:化学工业出版社, 2015.
TAO T X.Engineering Chemistry Experiment[M].Beijing:Chemical Industry Press, 2015.
[15] CHEN A N, GUAN Y J, BUSTAMANTE M, et al.Production of renewable fuel and value-added bioproducts using pineapple leaves in Costa Rica[J].Biomass and Bioenergy, 2020, 141:105675.
[16] JOHNSTON J H, BARFORD J P.Continuous growth of Saccharomyces cerevisiae on a mixture of glucose and fructose[J].The Journal of General and Applied Microbiology, 1991, 37(2):133-140.
[17] BERTHELS N J, CORDERO OTERO R R, BAUER F F, et al.Correlation between glucose/fructose discrepancy and hexokinase kinetic properties in different Saccharomyces cerevisiae wine yeast strains[J].Applied Microbiology and Biotechnology, 2008, 77(5):1083-1091.
[18] CRAGO C L, KHANNA M, BARTON J, et al.Competitiveness of Brazilian sugarcane ethanol compared to US corn ethanol[J].Energy Policy, 2010, 38(11):7404-7415.
[19] 杨铮. 我国乙醇产业发展概述[J].化学工业, 2021, 39(4):44-48;75.
YANG Z.A review of ethanol industry in China[J].Chemical Industry, 2021, 39(4):44-48;75.
[20] 郭孝孝, 武国庆.玉米燃料乙醇技术进展[J].生物产业技术, 2017(1):95-99.
GUO X X, WU G Q.Technology progress of global fuel ethanol industry[J].Biotechnology & Business, 2017(1):95-99.
