Food and Fermentation Industries >

2023 , Vol. 49 >Issue 13: 266 - 271

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034835

An indirect competitive enzyme-linked immunosorbent assay for determination of indomethacin and acemetacin in health food

  • LIU Fengyin ,
  • LU Manzhi ,
  • LIANG Shuiyuan ,
  • HU Yulu ,
  • HUAGN Gengsheng ,
  • ZOU Hongli ,
  • LI Gongke
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  • 1(College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China)
    2(School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China)
    3(College of Agriculture, Shanxi Agricultural University, Taigu 030801, China)

Received date: 2023-01-06

  Revised date: 2023-02-21

  Online published: 2023-08-07

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Abstract

This study focused on the illegal addition of indomethacin and acemetacin in health food. Indomethacin as an immune hapten was coupled to bovine serum albumin to produce immunogen, and acemetacin as a coating hapten was coupled to ovalbumin to produce coating antigen. New Zealand white rabbits were immunized and a polyclonal antibody was obtained which could recognize both indomethacin and acemetacin. Then an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for simultaneously detecting indomethacin and acemetacin residues in antirheumatic pain relieving health food samples was developed. The ic-ELISA demonstrated a half inhibition concentration (IC50) of 5.6 ng/mL for indomethacin and 7.4 ng/mL for acemetacin, respectively. The linear ranges were 0.9-32.9 ng/mL with a limit of detection (LOD) of 0.3 ng/mL for indomethacin, and 2.2-24.3 ng/mL with a LOD of 1.1 ng/mL for acemetacin. No cross-reactivity was observed to all the non-steroidal anti-inflammatory drugs tested. The antirheumatic pain relieving health food in tablets, hard capsules and soft capsules were extracted with methanol and the matrix effect was eliminated by diluting with standard solutions. The average recoveries of indomethacin and acemetacin in spiked samples were 80.0%-119.7%, with relative standard deviation below 15%, respectively. Good correlation between the ic-ELISA and liquid chromatography-tandem mass spectrometry was obtained for spiked health food samples, and the correlation coefficient were greater than 0.99. This proposed ic-ELISA was suitable for rapid screening for indomethacin and acemetacin in health food samples.

Key words： indomethacin; acemetacin; anti-rheumatic; health food; indirect competitive enzyme-linked immunosorbent assay(ic-ELISA); antibody

Cite this article

LIU Fengyin , LU Manzhi , LIANG Shuiyuan , HU Yulu , HUAGN Gengsheng , ZOU Hongli , LI Gongke . An indirect competitive enzyme-linked immunosorbent assay for determination of indomethacin and acemetacin in health food[J]. Food and Fermentation Industries, 2023 , 49(13) : 266 -271 . DOI: 10.13995/j.cnki.11-1802/ts.034835

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