Dynamic changes of flavor substances in pericarps of three cultivars of Zanthoxylum bungeanum with growth and development in Wudu areas of Bailongjiang River

  • HOU Yingying ,
  • MA Junyi ,
  • ZHU Jianzhao ,
  • HU Cuilan ,
  • HUANG Panpan ,
  • ZHAO Lin
Expand
  • 1(College of Life Science, Northwest Normal University, Lanzhou 730070, China)
    2(Institute of Walnut, Longnan Economic Forest Research Institute, Wudu 746000, China)

Received date: 2021-07-29

  Revised date: 2022-06-04

  Online published: 2023-08-07

Abstract

Three Zanthoxylum bungeanum (Sichuan pepper) cultivars of Shizitou, Maowen Dahongpao, and Shisheng Dahongpao from the Germplasm Bank of Longnan Economic Forest Research Institute in Gansu Province were used as the test materials. Reversed phase-high performance liquid chromatography (RP-HPLC) was used to detect numb-taste substances. Electronic nose and gas chromatography-mass spectrometry (GC-MS) were used to detect and analyze volatile substances. The dynamic changes of flavor substances in pericarps of three cultivars of Z. bungeanum were analyzed during growth and development. At the same time, numb-taste substances and volatile substances were used as the evaluation indexes to compare and analyze the quality of the three cultivars. Results showed that the contents of numb-taste substances in the pericarps of the three cultivars increased with the fruit ripening during the growth and development, showing a trend of continuous accumulation, which was consistent with the local harvest time. The contents of numb-taste substances from high to low were Maowen Dahongpao, Shizitou, and Shisheng Dahongpao. The electronic nose sensors of W2W, W1W, W2S, W1S, and W5S exhibited good responses to volatile substances from the three cultivars. There were seven main common volatile substances in Shizitou, Maowen Dahongpao, and Shisheng Dahongpao during their growth and development. Linalool, limonene, and 2-aminobenzoic acid-3,7-dimethyl-1,6-octandien-3-ester were detected continuously with high relative contents. The dynamic changes of volatile substance contents in the three kinds of Z. bungeanum were significantly different. Based on the principal component analysis, and using numb-taste substances and volatile substances in the maturity stage as the quality evaluation index, the quality of the three cultivated peppers was in the order of Maowen Dahongpao, Shizitou, and Shisheng Dahongpao.

Cite this article

HOU Yingying , MA Junyi , ZHU Jianzhao , HU Cuilan , HUANG Panpan , ZHAO Lin . Dynamic changes of flavor substances in pericarps of three cultivars of Zanthoxylum bungeanum with growth and development in Wudu areas of Bailongjiang River[J]. Food and Fermentation Industries, 2023 , 49(13) : 297 -303 . DOI: 10.13995/j.cnki.11-1802/ts.028593

References

[1] 边甜甜, 司昕蕾, 曹瑞, 等.花椒挥发油提取、成分分析及药理作用研究概述[J].中国中医药信息杂志, 2018, 25(8):129-132.
BIAN T T, SI X L, CAO R, et al.Review of extraction, component analysis and pharmacological effects of volatile oil in Zanthoxyli pericarpium[J].Chinese Journal of Information on Traditional Chinese Medicine, 2018, 25(8):129-132.
[2] 朱妞. 花椒活性成分研究现状及发展前景[J].粮食与油脂, 2020, 33(4):4-6.
ZHU N.Current situation and development prospect of active components of Zanthoxylum bungeanum Maxim[J].Cereals & Oils, 2020, 33(4):4-6.
[3] NOOREEN Z, TANDON S, YADAV N P, et al.Zanthoxylum:A systematic review of its traditional uses, naturally occurring constituents and pharmacological properties[J].Current Organic Chemistry.2019, 23(12):1307-1341.
[4] 王秋亚, 景晓卉.花椒精油化学成分、提取方法及抑菌活性研究进展[J].中国调味品, 2018, 43(12):187-190;195.
WANG Q Y, JING X H.Research progress of the chemical compositions, extraction methods and antibacterial activities of essential oil from Zanthoxylum bungeanum[J].China Condiment, 2018, 43(12):187-190;195.
[5] 赵二劳, 徐未芳, 刘乐, 等.花椒抑菌作用研究进展[J].中国调味品, 2019, 44(3):185-188.
ZHAO E L, XU W F, LIU L, et al.Research progress of the bacteriostasis of Zanthoxylum bungeanum[J].China Condiment, 2019, 44(3):185-188.
[6] 郭静, 阚建全.高效液相色谱(HPLC)定量检测花椒麻味物质含量方法的建立[J].中国食品添加剂, 2019, 30(2):142-147.
GUO J, KAN J Q.Determination of the numb-taste components from Zanthoxylum by HPLC[J].China Food Additives, 2019, 30(2):142-147.
[7] 杨清山, 翟彦伟, 栗星, 等.花椒及其提取物中花椒麻素的HPLC测定方法[J].食品工业, 2019, 40(8):328-332.
YANG Q S, ZHAI Y W, LI X, et al.The determination method of sanshool in Sichuan pepper and its extract by HPLC[J].The Food Industry, 2019, 40(8):328-332.
[8] ZHANG X X, ZHOU X Q, XI Z Y, et al.Surfactant-assisted enzymatic extraction of the flavor compounds from Zanthoxylum bungeanum[J].Separation Science and Technology, 2020, 55(9):1667-1676.
[9] 朱羽尧, 张国琳, 钱骅, 等.采收后加工对大红袍花椒中芳香成分和麻味物质含量及组成的影响[J].中国调味品, 2018, 43(10):74-80.
ZHU Y Y, ZHANG G L, QIAN H, et al.Effect of post-harvest processing on the content and composition of aromatic components and pungent substances in Zanthoxylum bungeanum[J].China Condiment, 2018, 43(10):74-80.
[10] ZHU L, WANG L, CHEN X, et al.Comparative studies on flavor substances of leaves and pericarps of Zanthoxylum bungeanum Maxim.at different harvest periods[J].Tropical Journal of Pharmaceutical Research, 2019, 18(2):279.
[11] 张凤芳, 马力.花椒麻度量化分级及麻味食品生产工艺研究[J].中国调味品, 2015, 40(9):72-74;80.
ZHANG F F, MA L.Study on quantitative and classification of numb-taste degree of pepper and production process of numb-taste food[J].China Condiment, 2015, 40(9):72-74;80.
[12] FEI X T, SHI Q Q, YANG T X, et al.Expression stabilities of ten candidate reference genes for RT-qPCR in Zanthoxylum bungeanum maxim[J].Molecules (Basel, Switzerland), 2018, 23(4):802.
[13] ZHANG L L, ZHAO L, WANG H Y, et al.The relationship between alkylamide compound content and pungency intensity of Zanthoxylum bungeanum based on sensory evaluation and ultra-performance liquid chromatography-mass spectrometry/ mass spectrometry (UPLC-MS/MS) analysis[J].Journal of the Science of Food and Agriculture, 2019, 99(4):1475-1483.
[14] 阚建全, 陈科伟, 任廷远, 等.花椒麻味物质的生理作用研究进展[J].食品科学技术学报, 2018, 36(1):11-17;44.
KAN J Q, CHEN K W, REN T Y, et al.Review on physiological function of alkylamide compounds from Zanthoxylum bungeanum[J].Journal of Food Science and Technology, 2018, 36(1):11-17;44.
[15] 肖志强, 李小军, 焦素慧, 等.陇南山区特色农业气候适宜性区划研究[J].现代农业科技, 2017(21):215-216;219.
XIAO Z Q, LI X J, JIAO S H, et al.Study on climatic suitability regionalization of characteristic agriculture in Longnan mountainous area[J].Modern Agricultural Science and Technology, 2017(21):215-216;219.
[16] 张正武, 王勃, 林云, 等.基于电子鼻技术的陇南花椒品种区分[J].经济林研究, 2019, 37(4):188-193.
ZHANG Z W, WANG B, LIN Y, et al.Identification of Longnan pepper cultivars based on electronic nose technology[J].Non-Wood Forest Research, 2019, 37(4):188-193.
[17] 陈茜, 陶兴宝, 黄永亮, 等.花椒香气研究进展[J].中国调味品, 2018, 43(1):189-194.
CHEN Q, TAO X B, HUANG Y L, et al.Research progress on the aroma of Chinese prickly ash[J].China Condiment, 2018, 43(1):189-194.
[18] JIANG L H, KUBOTA K.Differences in the volatile components and their odor characteristics of green and ripe fruits and dried pericarp of Japanese pepper (Xanthoxylum piperitum DC.)[J].Journal of Agricultural and Food Chemistry, 2004, 52(13):4197-4203.
[19] LIU Y L, WEI A Z.The complete chloroplast genome sequence of an economically important plant, Zanthoxylum bungeanum (Rutaceae)[J].Conservation Genetics Resources, 2017, 9(1):25-27.
[20] HU Y, TIAN L, SHI J W, et al.Genetic structure of cultivated Zanthoxylum species investigated with SSR markers[J].Tree Genetics & Genomes, 2018, 14(6):89.
[21] FENG S J, LIU Z S, CHEN L, et al.Phylogenetic relationships among cultivated Zanthoxylum species in China based on cpDNA markers[J].Tree Genetics & Genomes, 2016, 12(3):45.
[22] 乐薇, 吴士筠, 高欣.大红袍花椒挥发油的提取及化学成分的气相色谱-质谱分析[J].食品科学, 2014, 35(2):261-265.
LE W, WU S J, GAO X.Extraction and composition analysis by gas chromatography-mass spectrometry of volatile oil from Zanthoxylum bungeanum Maxim[J].Food Science, 2014, 35(2):261-265.
[23] 王利平, 李占杰.陕西韩城大红袍花椒挥发油化学成分的研究[J].食品工业科技, 2003(12):20-23.
WANG L P, LI Z J, Study on the chemical constituents of the volatile oil of Zanthoxylum bungeanum from Hancheng Dahongpao, Shanxi Province[J].Science and Technology of Food Industry, 2003(12):20-23.
[24] 乔明锋, 刘阳, 袁小钧, 等.茂县花椒化学成分分析及抑菌活性研究[J].中国调味品, 2017, 42(4):59-63;73.
QIAO M F, LIU Y, YUAN X J, et al.Study on chemical constituents and antibacterial activity of Zanthoxylum bungeanum in Maoxian[J].China Condiment, 2017, 42(4):59-63;73.
[25] 霍归国, 梁婷玉, 史金涵, 等.武都‘大红袍'花椒品质研究[J].中国农学通报, 2021, 37(22):120-125.
HUO G G, LIANG T Y, SHI J H, et al.Study on the quality of Zanthoxylum bungeanum ‘Dahongpao' from Wudu[J].Chinese Agricultural Science Bulletin, 2021, 37(22):120-125.
Outlines

/