Food and Fermentation Industries >

2023 , Vol. 49 >Issue 14: 25 - 34

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033241

Isolation, purification, structure, and functional activity of the exopolysaccharides from a space mutant of Leuconostoc enterica

  • PENG Jiayi ,
  • LI Yao ,
  • ZHANG Mengyu ,
  • WANG Qingqing ,
  • ZHONG Qingping
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  • (Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)

Received date: 2022-08-08

  Revised date: 2022-09-25

  Online published: 2023-08-30

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Abstract

A space mutation strain of Leuconostoc enterica L21-49 that is of high-yield of exopolysaccharide (EPS) was token as the research object, its EPSs was extracted and purified by sequential column chromatography on DEAE-Sepharose Fast Flow and Sephadex G-200. The molecular mass and the structure of the purified EPSs were determined by gel permeation chromatography, infrared spectroscopy and liquid chromatography. Meanwhile, the anti-biofilm, anti-oxidation, and the inhibitory activities on α-amylase and α-glucosidase were analyzed. The results showed that EPS-A, EPS-B, and EPS-C were purified from the exopolysaccharides produced by L21-49, with the relative molecular weights of 60 629, 36 959 and 24 714 Da, respectively. EPS-A mainly contained mannose and glucose, EPS-B mainly contained mannose and galactose, and EPS-C mainly contained glucose and galactose. In vitro functional activity studies showed that the purified EPSs exhibited good inhibitory activities against pathogenic biofilms. The antioxidant capacity and inhibitory activities on α-amylase and α-glucosidase of EPS-C were higher than those of EPS-A and EPS-B, it might be related to its molecular weight and functional groups. The findings might be of positive significance for the development and utilization of the EPSs of lactic acid bacteria.

Key words： lactic acid bacteria; exopolysaccharide; separation and purification; structural analysis; functional activity

Cite this article

PENG Jiayi , LI Yao , ZHANG Mengyu , WANG Qingqing , ZHONG Qingping . Isolation, purification, structure, and functional activity of the exopolysaccharides from a space mutant of Leuconostoc enterica[J]. Food and Fermentation Industries, 2023 , 49(14) : 25 -34 . DOI: 10.13995/j.cnki.11-1802/ts.033241

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