A mixture of glucose and sophorose (MGD) as developed from glucose through the transglycosylation reaction catalyzed by β-glucosidase, resulting in the overproduction of cellulase by Trichodema reesei Rut C30. However, proteomics showed that the significantly low expression of the SWO1 gene resulted in a low efficiency of cellulase hydrolysis compared with that of cellulose as an inducer. Therefore, the endogenous Trswo1 gene was overexpressed in T. reesei Rut C30 using a constitutive promoter pyruvate decarboxylase 1(PDC1), and the SWO1 transcription levels of the two transformants T. reesei OEswo1-1 and OEswo1-2 were increased by 4.65 times and 3.91 times, respectively. There was no significant change in filter paper activity, endoglucanases, or cellobiohydrolases, but the crude enzymes of T. reesei OEswo1-1 and OEswo1-2 were applied to hydrolyze corn stover with a solid loading of 5%, and the hydrolysis efficiencies were increased by 6.98% and 13.93%, respectively. Furthermore, the X-ray diffraction (XRD) and the scanning electron microscope demonstrated that the enhanced hydrolysis efficiency was primarily triggered by the decrease in the crystallinity of lignocellulose. Furthermore, the fiber structure exhibited varying degrees of loosening and disintegration. The results of this study have implications for the low content of auxiliary protein that degrade cellulose in cellulase systems synthesized by T. reesei with MGD as an inducer.
MO Yi
,
RAN Xiaoqin
,
WANG Zancheng
,
CHEN Yudian
,
PENG Nian
,
LI Jianghong
,
LI Yonghao
. Over-expressing of the endogenous Trswo1 gene in Trichoderma reesei to increase the cellulose degrading capability[J]. Food and Fermentation Industries, 2023
, 49(14)
: 60
-66
.
DOI: 10.13995/j.cnki.11-1802/ts.033370
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