The utilization of carbohydrates by Bifidobacterium has always been a research focus, but the overall study on the utilization ability of carbohydrates by Bifidobacterium pseudocatenulatum is still lacking. In order to deepen the understanding of carbohydrate utilization in B. pseudocatenulatum, the growth curve and in vitro fermentation experiments were used to study the utilization ability of 12 strains of B. pseudocatenulatum to 17 carbohydrates. B. pseudocatenulatum can achieve high biomass in glucose, lactose, galactooligosaccharides (GOS) medium (OD600≥0.99), and can reach a high growth rate (generation time≤150 min) in sucrose and GOS medium. The study also revealed significant inter-strain differences in carbohydrate utilization among the 12 strains, with FJSNT36M3 utilizing only five carbohydrates, while three strains (FHNBA14M1, FGZ16I1M1, FXJWS49M21) utilize more than 10 carbohydrates. On the generation time and OD600 indicator, three strains (FGSYC12M4, FGZ16I1M1, FFJND17M1) has a strong ability to utilize nine kinds of carbohydrates. Most of the glycoside hydrolases encoded by the 12 strains of B. pseudocatenulatum degrade plant-derived carbohydrates. The differences in carbohydrate utilization among strains may due to the differences in the enzymes that degrade and transport carbohydrates. In vitro fermentation experiments of human feces proves that the difference in carbohydrate utilization ability of B. pseudocatenulatum relates to its potential to occupy abundance in gut.
[1] TURRONI F, FORONI E, PIZZETTI P, et al.Exploring the diversity of the bifidobacterial population in the human intestinal Tract[J].Applied and Environmental Microbiology, 2009, 75(6):1 534-1 545.
[2] 金红芝, 范小兵, 杭晓敏, 等.儿童肠道双歧杆菌和乳杆菌种群结构分析[J].微生物学报, 2005,45(4):567-570.
JIN H Z, FAN X B, HANG X M, et al.Analysis of the probiotic Bifidobacterium and Lactobacillus community in child intestinal flora[J].Acta Microbiologica Sinica, 2005, 45(4):567-570.
[3] 张敏, 范小兵, 杭晓敏, 等.青年人肠道菌群分布及关键益生菌群落结构分析[J].微生物学报,2004,44(5):621-626.
ZHANG M, FAN X B, HANG X M, et al.Distribution of youth intestinal flora and analysis of key probiotics community[J].Acta Microbiologica Sinica, 2004, 44(5):621-626.
[4] 张和春, 王劲松, 杨国, 等.健康老年人肠道双歧杆菌和乳杆菌种群多样性分析[J].中国微生态学杂志, 2006, 18(6):446-448.
ZHANG H C, WANG J S, YANG G, et al.Analysis of the probiotics Bifidobacterium and Lactobacillus community in healthy elderly intestinal flora[J].Chinese Journal of Microecology, 2006, 18(6):446-448.
[5] SANCHIS-CHORDÁ J, DEL PULGAR E M G, CARRASCO-LUNA L J, et al.Bifidobacterium pseudocatenulatum CECT 7765 supplementation improves inflammatory status in insulin-resistant obese children[J].European Journal of Nutrition, 2019, 58(7):2 789-2 800.
[6] 吴欢, 王睿瑞, 赵宇峰, 等.一名肥胖儿童营养干预后肠道内假小链双歧杆菌碳水化合物利用能力的探究[J].基因组学与应用生物学, 2015, 34(7):1 384-1 391.
WU H, WANG R R, ZHAO Y F.Exploring carbohydrate utilization capacity of Bifidobacterium pseudocaten ulatum isolated from a morbidly obese child after dietary intervention[J].Genomics and Applied Biology, 2015, 34(7):1 384-1 391.
[7] 薛欣合, 翟芮, 张晨虹.一株人源假小链双歧杆菌缓解小鼠 DSS 致溃疡性结肠炎[J].基因组学与应用生物学, 2019, 38(10):4 520-4 529.
XUE X H, ZHAI R, ZHANG C H.Amelioration effects of one human Bifidobacterium pseudocatenulatum strain on murine DSS-induced colitis[J].Genomics and Applied Biology, 2019, 38(10):4 520-4 529.
[8] CHEN Y, YANG B, STANTON C, et al.Bifidobacterium pseudocatenulatum ameliorates DSS-induced colitis by maintaining intestinal mechanical barrier, blocking proinflammatory cytokines, inhibiting TLR4/NF-κ signaling, and altering gut microbiota[J].Journal of Agricultural and Food Chemistry, 2021, 69(5):1 496-1 512.
[9] GARCÍA-MANTRANA M I, MONEDERO V, HAROS M.Myo-inositol hexakisphosphate degradation by Bifidobacterium pseudocatenulatum ATCC 27919 improves mineral availability of high fibre rye-wheat sour bread[J].Food Chemistry, 2015, 178:267-275.
[10] FINEGOLD S M, SUTTER V L, MATHISEN G E.Normal Indigenous Intestinal Flora[M].Human Intestinal Microflora in Health and Disease. Amsterdam: Elsevier, 1983, 1:3-31.
[11] POKUSAEVA K, FITZGERALD G F, VAN SINDEREN D.Carbohydrate metabolism in bifidobacteria[J].Genes & Nutrition, 2011, 6(3):285-306.
[12] SCHELL M A, KARMIRANTZOU M, SNEL B, et al.The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract[J].Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(22):14 422-14 427.
[13] MILANI C, TURRONI F, DURANTI S, et al.Genomics of the genus Bifidobacterium reveals species-specific adaptation to the glycan-rich gut environment[J].Applied and Environmental Microbiology, 2015, 82(4):980-991.
[14] ALEGRÍA Á, DELGADO S, GUADAMURO L, et al.The genome of Bifidobacterium pseudocatenulatum IPLA 36007, a human intestinal strain with isoflavone-activation activity[J].Gut Pathogens, 2014, 6:31.
[15] WU G J, ZHANG C H, WU H, et al.Genomic microdiversity of Bifidobacterium pseudocatenulatum underlying differential strain-level responses to dietary carbohydrate intervention[J].mBio, 2017, 8(1):e02 348-e02 316.
[16] 刘时捷. 双歧杆菌碳源利用特性及种间互养机制研究[D].无锡:江南大学,2021.
LIU S J.Study on utilization characteristics of carbon source and interspecific mutualism mechanism of Bifidobacterium[D].Wuxi:Jiangnan University, 2021.
[17] GOODMAN A L, KALLSTROM G, FAITH J J, et al.Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice[J].Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(15):6 252-6 257.
[18] LIKOTRAFITI E, TUOHY K M, GIBSON G R, et al.An in vitro study of the effect of probiotics, prebiotics and synbiotics on the elderly faecal microbiota[J].Anaerobe, 2014, 27:50-55.
[19] MAO B Y, YIN R M, LI X S, et al.Comparative genomic analysis of Lactiplantibacillus plantarum isolated from different niches[J].Genes, 2021, 12(2):241.
[20] 肖越. 长双歧杆菌肠道定殖的研究[D].无锡:江南大学,2021.
XIAO Y.Study on intestinal colonization of Bifidobacterium longum[D].Wuxi:Jiangnan University, 2021.
[21] ISHIZUKA A, TOMIZUKA K, AOKI R, et al.Effects of administration of Bifidobacterium animalis subsp.lactis GCL2505 on defecation frequency and bifidobacterial microbiota composition in humans[J].Journal of Bioscience and Bioengineering, 2012, 113(5):587-591.
[22] 张英春. 益生菌功能特性及共生性研究[D].哈尔滨:东北农业大学,2003.
ZHANG Y C.Study on functional characteristics and symbiosis of probiotics[D].Haerbin:Northeast Agricultural University, 2003.
[23] ZHU Y T, ZHANG S L, MA Q, et al.Global genome and comparative transcriptomic analysis reveal the inulin consumption strategy of Lactiplantibacillus plantarum QS7T[J].Food Research International, 2022, 151:110846.
[24] POKUSAEVA K, FITZGERALD G F, VAN SINDEREN D.Carbohydrate metabolism in bifidobacteria[J].Genes and Nutrition, 2011, 6(3):285-306.
[25] SONNENBURG J L, BÄCKHED F.Diet-microbiota interactions as moderators of human metabolism[J].Nature, 2016, 535(7610):56-64.
