Food and Fermentation Industries >

2023 , Vol. 49 >Issue 14: 110 - 117

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035417

Dynamic quorum-sensing regulation to enhance the production of salidroside in Escherichia coli

  • SHEN Yuping ,
  • HE Xi ,
  • ZHOU Ziwei ,
  • HE Chunlan ,
  • ZHANG Zujiao
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  • 1(College of chemistry and bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China)
    2(Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling area, Yongzhou 425199, China)
    3(Hunan Provincial Engineering Research Center for Ginkgo Biloba, Yongzhou 425199, China)

Received date: 2023-03-09

  Revised date: 2023-03-28

  Online published: 2023-08-30

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Abstract

Salidroside is a high value-added natural product with great potential of application and development. However, the current de novo synthesis of salidroside in Escherichia coli suffers from poor yield and high proportion of intermediate tyrosol. Combination of the phenylpyruvate decarboxylase mutant ARO10F138L//D218G obtained in our previous study, and screening of the key enzymes of alcohol dehydrogenase and uridine diphosphate glucose glycosyltransferase in salidroside synthesis pathway, the titer of salidroside reached up to (605.8±16.8) mg/L. Furthermore, the titer of salidroside was increased by 95.0% to (1 156.3±44.2) mg/L with deletion of feaB and overexpression of pgm and galU for enhancing supply of uridine diphosphate glucose. To overcome the problems of high accumulation of intermediate tyrosol and low cell density in salidroside fermentation, a quorum-sensing circuit was used to dynamically control the salidroside synthesis pathway, which alleviated the toxic effect of tyrosol on chassis cells and lowered the proportion of tyrosol successfully. To this end, the titer of salidroside was increased by 36.3% to (1 484.6±21.4) mg/L, the OD600 increased by 31.6 % to 8.18±0.23. In a 2 L fermenter, the production of salidroside in the engineered strain dynamically regulated by quorum-sensing reached 4.38 g/L with an OD600 of 46.74. Compared with those in the engineered strain statically regulated by induced expression, the yield of salidroside was increased by 48.2%, the OD600 was enhanced by 26.6%, and the proportion of tyrosol in fermentation broth was also reduced from 33.7% to 17.9%, which showed great potential for production of salidroside. In the current study, the results revealed that dynamic quorum-sensing regulation could effectively reduce the accumulation of intermediates and alleviate the inhibitory effect of metabolite toxicity on chassis cells. This study has important reference significance and great application value for the biosynthesis of other glycoside natural products or chemicals with high toxicity.

Key words： salidroside; Escherichia coli; quorum sensing; dynamic control; alcohol dehydrogenase; uridine diphosphate glucose glycosyltransferase

Cite this article

SHEN Yuping , HE Xi , ZHOU Ziwei , HE Chunlan , ZHANG Zujiao . Dynamic quorum-sensing regulation to enhance the production of salidroside in Escherichia coli[J]. Food and Fermentation Industries, 2023 , 49(14) : 110 -117 . DOI: 10.13995/j.cnki.11-1802/ts.035417

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