Food and Fermentation Industries >

2023 , Vol. 49 >Issue 14: 132 - 137

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032593

Cysteine alleviates cadmium-induced disorders of hepatic lipid metabolism in mice by inhibiting oxidative stress and inflammation

  • WANG Jingwen ,
  • FANG Zhijia ,
  • GAO Yuan ,
  • GUAN Wenhao ,
  • SUN Lijun ,
  • LIU Ying
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  • (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)

Received date: 2022-06-07

  Revised date: 2022-07-15

  Online published: 2023-08-30

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Abstract

To explore the protective effect and mechanism of cysteine (Cys) on cadmium-induced liver injury in mice. SPF Kunming mice were divided into control group, cadmium (Cd) group (intraperitoneal injection of cadmium chloride), cadmium and low-dose Cys co-treatment group (intraperitoneal injection of cadmium chloride plus oral administration of low-dose Cys), cadmium and high-dose Cys co-treatment group (intraperitoneal injection of cadmium chloride plus oral administration of high-dose Cys). After the treatment, the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum and the contents of total cholesterol (TC) and triacylglycerol (TG) in liver tissue were determined; The pathological changes of liver tissue were observed via HE staining. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase (CAT) in liver tissue were detected by microvolume assay; The levels of IL-6, IL-1β and TNF-α in liver were detected by ELISA. The expression levels of genes related to lipid metabolism in liver were measured by qRT-PCR. The results showed that the activities of serum ALT and AST were significantly increased in Cd-exposed mice compared to the control group (P<0.01). The liver tissue had obvious pathological changes and the contents of TC and TG in the liver were significantly increased (P<0.01). The level of MDA in liver tissue was significantly increased, and the activities of SOD and CAT were significantly decreased (P<0.01). The supplementation of Cys can significantly improve the adverse changes of the indicators above (P<0.05 or P<0.01). In addition, Cys supplementation enhanced anti-inflammatory potential during Cd exposure and normalized pro-inflammatory cytokine levels. Cys also normalized the expression of Cd-disturbed lipid metabolism-related genes to improve lipid accumulation, which helped reduce liver damage. The treatment of Cys reduced oxidative stress and inflammatory responses, which may help to prevent Cd-induced lipid metabolism disorders and liver injury.

Key words： cadmium; liver injury; inflammatory factors; oxidative damage; lipid metabolism

Cite this article

WANG Jingwen , FANG Zhijia , GAO Yuan , GUAN Wenhao , SUN Lijun , LIU Ying . Cysteine alleviates cadmium-induced disorders of hepatic lipid metabolism in mice by inhibiting oxidative stress and inflammation[J]. Food and Fermentation Industries, 2023 , 49(14) : 132 -137 . DOI: 10.13995/j.cnki.11-1802/ts.032593

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