Food and Fermentation Industries >

2023 , Vol. 49 >Issue 15: 145 - 152

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032423

Characteristics of collagens from body wall of Stichopus horrens prepared by different extraction methods

  • ZHENG Qingyao ,
  • CAO Wenhong ,
  • HAN Yuliang ,
  • ZHANG Fengning ,
  • CHEN Zhongqin ,
  • LIN Haisheng ,
  • ZHENG Huina
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  • 1(College of Food Science and Technology, Guangdong Ocean University, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2022-05-23

  Revised date: 2022-06-17

  Online published: 2023-08-31

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Abstract

To investigate the effects of different extraction methods on the molecular structure and physicochemical properties of collagens from the body wall of Stichopus horrens, acid-soluble collagen (ASC), pepsin-solubilized collagen (PSC), ultrasound-assisted extraction of acid-soluble collagen (UASC), and ultrasound-assisted extraction of pepsin-solubilized collagen (UPSC) were extracted from the body wall of S. horrens, the extraction rate, SDS-PAGE, ultraviolet spectrum(UV), Fourier transform infrared spectrum (FTIR), thermal stability and circular dichroism spectrum (CD) of different extraction methods were compared and analyzed. Results showed that the extraction rate of UPSC was the highest (18.70%) and that of ASC was only 0.15%, which was not used in subsequent studies. SDS-PAGE showed that the subunit composition of PSC, UPSC, and UASC was (α1)2α2. FTIR spectrum showed that there were amide A, B, Ⅰ, Ⅱ, and Ⅲ bands in all three kinds of collagens, which maintained the integrity of the triple helix structure of collagens, after ultrasonic treatment, the contents of α-helix decreased, while the contents of β-pleated sheet and β-turn increased. The thermal denaturation temperatures of PSC, UPSC, and UASC were 21.3 ℃, 25.9 ℃, and 27.0 ℃, respectively. CD and UV spectrum showed that three kinds of collagens were consistent with the typical structural characteristics of type Ⅰ collagen. In conclusion, it could preliminarily be judged that the collagens from the body wall of S. horrens were type Ⅰ collagen, ultrasonic-assisted extraction did not destroy the triple helix structure of collagens, it could promote the extraction of collagens. This study could provide basic data for the further development and utilization of collagens from the body wall of S. horrens.

Key words： Stichopus horrens; collagen; extraction methods; structure characteristics; physicochemical properties

Cite this article

ZHENG Qingyao , CAO Wenhong , HAN Yuliang , ZHANG Fengning , CHEN Zhongqin , LIN Haisheng , ZHENG Huina . Characteristics of collagens from body wall of Stichopus horrens prepared by different extraction methods[J]. Food and Fermentation Industries, 2023 , 49(15) : 145 -152 . DOI: 10.13995/j.cnki.11-1802/ts.032423

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