In this study, the Mytilus edulis shell was treated with NaOH-lactic acid. The effects of the pH and immersion time of NaOH and lactic acid solution on the removal of cuticles on Mytilus edulis shell were investigated by using the mass loss rate and the brightness of the shell powder as the indicators. It was found that the optimal treatment conditions were as follows: immersing in NaOH solution (pH 13) for 36 h, and then immersing in lactic acid solution (pH 2) for 4 h. The results of scanning electron microscope, Fourier transform infrared spectroscopy, and inductively coupled plasma mass spectrometry analysis showed that NaOH-lactic acid treatment effectively removed the melanin and the impurified CaCO3 adjoining with the cuticles on the shell, and also the elements adsorbed on the cuticles, such as Br, Zn, and Pb. The mass ratio of Ca of M. edulis shell surface was increased from 0.39% in control to 37.58% after NaOH-lactic acid treatment, which was approximately equal to the theoretical value in CaCO3. This study provides a reference for the preparation of high-quality calcium supplements from the M. edulis shell.
KE Zhigang
,
CHEN Jinyu
,
XIANG Xingwei
,
CHEN Hui
,
ZHOU Xuxia
,
JIN Youding
,
DAI Yangzhang
,
DENG Shanggui
,
ZHOU Xiaomin
,
DING Yuting
,
LIU Shulai
. Removal of cuticle on Mytilus edulis shell by sodium hydroxide-lactic acid treatment[J]. Food and Fermentation Industries, 2023
, 49(15)
: 208
-214
.
DOI: 10.13995/j.cnki.11-1802/ts.032382
[1] GRIENKE U, SILKE J, TASDEMIR D.Bioactive compounds from marine mussels and their effects on human health[J].Food Chemistry, 2014, 142:48-60.
[2] ZHAN J X, LU J S, WANG D.Review of shell waste reutilization to promote sustainable shellfish aquaculture[J].Reviews in Aquaculture, 2022, 14(1):477-488.
[3] 叶剑, 徐仰丽, 张井, 等.海洋生物钙的开发利用研究进展[J].中国海洋药物, 2021, 40(5):71-78.
YE J, XU Y L, ZHANG J, et al.Research progress on exploitation and utilization of marine biological calcium[J].Chinese Journal of Marine Drugs, 2021, 40(5):71-78.
[4] LEE G W, YOON I S, LEE H J, et al.Properties of calcium lactate prepared from calcined littleneck clam Ruditapes philippinarum shell powder[J].Korean Journal of Fisheries and Aquatic Sciences, 2016, 49(4):436-444.
[5] 赵桂丰, 杨林, 陈雷, 等.微波高温加热法分解贝壳制取葡萄糖酸钙[J].大连工业大学学报, 2009, 28(6):438-440.
ZHAO G F, YANG L, CHEN L, et al.Production of calcium gluconate from shell by microwave high-temperature refining[J].Journal of Dalian Polytechnic University, 2009, 28(6):438-440.
[6] WANG Z, WANG K Y, FENG Y N, et al.Preparation, characterization of L-aspartic acid chelated calcium from oyster shell source and its calcium supplementation effect in rats[J].Journal of Functional Foods, 2020, 75:104249.
[7] YARRA T, RAMESH K, BLAXTER M, et al.Transcriptomic analysis of shell repair and biomineralization in the blue mussel, Mytilus edulis[J].BMC Genomics, 2021, 22(1):437.
[8] YAO Z T, XIA M S, LI H Y, et al.Bivalve shell:Not an abundant useless waste but a functional and versatile biomaterial[J].Critical Reviews in Environmental Science and Technology, 2014, 44(22):2502-2530.
[9] EPPLE M.Book review:Biomineralization principles and concepts in bioinorganic materials chemistry.by Stephen Mann[J].Angewandte Chemie International Edition, 2003, 42(4):381-382.
[10] 刘雅玫. 基于贻贝壳黑色素光热效应的抗菌活性对耐药性金黄色葡萄球菌感染伤口愈合的影响[D].青岛:青岛大学, 2020.
LIU Y M.Effect of antibacterial activity based on the photothermal effect of mussel shell melanin on wound healing of drug-resistant Staphylococcus aureus infection[D].Qingdao:Qingdao University, 2020.
[11] STEWART B D, JENKINS S R, BOIG C, et al.Metal pollution as a potential threat to shell strength and survival in marine bivalves[J].Science of the Total Environment, 2021, 755:143019.
[12] SONG J L, LIU G F, SONG Y H, et al.Positive effect of compound amino acid chelated calcium from the shell and skirt of scallop in an ovariectomized rat model of postmenopausal osteoporosis[J].Journal of the Science of Food and Agriculture, 2022, 102(4):1363-1371.
[13] 邵江娟, 王唯, 陈斌寅, 等.以牡蛎壳为钙源的L-天冬氨酸螯合钙的合成及其生物利用度研究[J].南京中医药大学学报, 2012, 28(4):371-373.
SHAO J J, WANG W, CHEN B Y, et al.Synthesis of L-aspartate chelating calcium with oysters as calcium source and investigation of its bioavailability[J].Journal of Nanjing University of Traditional Chinese Medicine, 2012, 28(4):371-373.
[14] XIE J W, LI H Y, CHE H X, et al.Extraction, physicochemical characterisation, and bioactive properties of ink melanin from cuttlefish (Sepia esculenta)[J].International Journal of Food Science & Technology, 2021, 56(7):3 627-3 640.
[15] MITITELU M, MOROŞAN E, NICOARĂ A C, et al.Development of immediate release tablets containing calcium lactate synthetized from black sea mussel shells[J].Marine Drugs, 2022, 20(1):45.
[16] VOICA C, DEHELEAN A, KOVACS M H, et al.The use of inductively coupled plasma mass spectrometry (ICP-MS) for the determination of toxic and essential elements in different types of food samples[C]//AIP Conference Proceedings.AIP, 2012.
[17] 邹德靓, 陈庆国, 杨平, 等.改性贻贝壳对亚甲基蓝的吸附去除研究[J].环境科学与技术, 2014, 37(12):214-217;252.
ZOU D L, CHEN Q G, YANG P, et al.Study on adsorption removal of methylene blue by modified mussel shell[J].Environmental Science & Technology, 2014, 37(12):214-217;252.
[18] FENG Q L, LI H B, PU G, et al.Crystallographic alignment of calcite prisms in the oblique prismatic layer of Mytilus edulis shell[J].Journal of Materials Science, 2000, 35(13):3337-3340.
[19] YANG X F.A self-constraint strengthening mechanism and its application to seashells[J].Journal of Materials Research, 1995, 10(6):1485-1490.
[20] 王芬, 余军霞, 肖春桥, 等.CO2碳化法制备微米级球霰石型食品碳酸钙的研究[J].硅酸盐通报, 2017, 36(1):43-50;56.
WANG F, YU J X, XIAO C Q, et al.Preparation of micro-size food-grade vaterite CaCO3 by CO2 carbonization method[J].Bulletin of the Chinese Ceramic Society, 2017, 36(1):43-50;56.
[21] CENTENO S A, SHAMIR J.Surface enhanced Raman scattering (SERS) and FTIR characterization of the sepia melanin pigment used in works of art[J].Journal of Molecular Structure, 2008, 873(1-3):149-159.
[22] TU Y G, SUN Y Z, TIAN Y G, et al.Physicochemical characterisation and antioxidant activity of melanin from the muscles of Taihe Black-bone silky fowl (Gallus Gallus domesticus Brisson)[J].Food Chemistry, 2009, 114(4):1345-1350.
[23] 周佑洲. 海水养殖中壳类废弃物的应用研究[D].杭州:浙江大学, 2018.
ZHOU Y Z.Study on application of shell waste from marine aquaculture[D].Hangzhou:Zhejiang University, 2018.
[24] 孙美玲, 冶育芳, 赵志西, 等.不同有机质对砷在针铁矿上吸附过程的影响[J].生态学杂志,2023,42(5):1115-1122.
SUN M L, YE Y F, ZHAO Z X, et al.Effects of different organic matter on the adsorption process of arsenic on goethite[J].Chinese Journal of Ecology, 2023,42(5):1115-1122.
