Research progress of short-chain primary alcohol oxidase

  • WANG Shoufeng ,
  • CONG Wenjie ,
  • CAO Xinchun ,
  • LU Ting ,
  • WANG Mingxuan ,
  • ZHOU Hualan ,
  • ZHANG Jianguo
Expand
  • (School of Health Science and Enginering, University of Shanghai for Science and Technology, Shanghai 200093, China)

Received date: 2022-07-29

  Revised date: 2022-09-08

  Online published: 2023-08-31

Abstract

Short-chain primary alcohol oxidase from eukaryotic methylotroph is a flavin adenine dinucleotide (FAD)-dependent enzyme that oxidizes short-chain primary alcohols to their corresponding aldehydes. SPAOX locates in peroxisomes of eukaryotes, which is a key enzyme involved in alcohol metabolism. SPAOX has been widely used in detection and biotransformation production due to its wide range of substrate and the recycle of FAD during the reaction. The multimer structure of SPAOX makes it vulnerable to environment and chemicals during the progress of reaction and storage, which limits its application. This review outlines the fundamental characteristics of SPAOX from the perspectives of source, catalytic mechanism and structure, substrate, and stability, puts a foundation for application and development, provides an outlook for future research based on molecular level, and built base for research related to SPAOX.

Cite this article

WANG Shoufeng , CONG Wenjie , CAO Xinchun , LU Ting , WANG Mingxuan , ZHOU Hualan , ZHANG Jianguo . Research progress of short-chain primary alcohol oxidase[J]. Food and Fermentation Industries, 2023 , 49(15) : 296 -304 . DOI: 10.13995/j.cnki.11-1802/ts.033144

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