Acne is an inflammatory skin condition of the sebaceous units of the hair follicles, with a prevalence of up to 85% in adolescents, which is associated with the colonization of Cutibacterium acnes. To obtain microorganisms that can efficiently antagonize C. acnes and enrich the resource base of C. acnes antagonists. Agar diffusion method was used to screen the strains with strong antibacterial activity against C. acnes from sauerkraut. The strain was identified by colony morphology, biochemical identification, and 16S rDNA gene sequence comparison. The antibacterial activity of the antagonistic bacteria was enhanced by optimizing the culture medium and fermentation conditions. In vitro safety evaluation methods were used to assess the safety of antagonistic bacteria. A strain HA2 was screened for its ability to efficiently antagonize C. acnes and was initially identified as Bacillus haynesii. By optimizing the fermentation conditions of strain HA2, the diameter of the fermentation supernatant inhibition circle was increased from 38 mm to 53 mm. According to the conversion of the tetracycline potency curve, the potency increased from 20 308.6 U to 112 927.6 U, resulting in a 5.56-fold increase. After in vitro safety evaluation, strain HA2 was initially judged to be a safe strain. The Bacillus haynesii HA2 is a strain that can efficiently antagonize C. acnes and has successfully passed the in vitro safety evaluation, which is valuable for further research.
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