A scientific, accurate, and efficient method for the determination of five prostaglandins (PG) in onions by liquid chromatography-tandem mass spectrometry was established. After ethanol extraction, the sample was absorbed with magnesium sulfate anhydrous, centrifuged on a high-speed refrigerated centrifuge and evaporated to dryness on a rotary evaporator. Finally, it was redissolved with methanol + acetonitrile, 5 mmol/L ammonium formate solution + acetonitrile + methanol as the mobile phase, gradient eluted, the flow rate was 0.2 mL/min, the column temperature was 35 ℃, and the injection volume was 2 μL. The extract was separated by a chromatographic column, and multi-reaction analysis was carried out by a four-pole mass spectrometer in the mode of electrospray spray anion. Within the range of experimental concentration, the standard curves of five PG were linear, and the correlation coefficient (R2) was greater than 0.999. At the concentration levels of 10, 20, and 40 ng/mL, the recoveries of the five PG were 96%-109%, the relative standard deviation was 0.26%-1.81%, and the detection limit and quantitative limit were 1.0 and 2.5 μg/kg. This method and the method reported in the literature were used to extract and analyze the samples and samples after spiking. The comparison of test data showed that this method had a better extraction effect, and the recovery rate of spiking was far higher than the method reported in the literature, which further proved the feasibility of this method. The results showed that the method was scientific, accurate, and easy to operate, and it could completely meet the analysis and determination of five PG in onion.
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