Food and Fermentation Industries >

2023 , Vol. 49 >Issue 17: 31 - 36

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035749

Identification and genome shuffling breeding of Saccharomyces cerevisiae CLNJ1 producing γ-aminobutyric acid

  • CHI Yanping ,
  • LU Yawen ,
  • WANG Jinghui ,
  • LI Da ,
  • SU Ying ,
  • NIU Honghong ,
  • MIAO Xinyu ,
  • SUN Mubai ,
  • HUA Mei
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  • (Institute of Argo-food Technology, Jilin Academy of Agricultural of Science, Changchun 130033, China)

Received date: 2023-04-07

  Revised date: 2023-05-04

  Online published: 2023-09-27

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Abstract

The breeding of Saccharomyces cerevisiae CLNJ1 producing high γ-aminobutyric acid(GABA) will provide theoretical basis and technical support for applying to food and pharmaceutical industries. The CLNJ1 was bred by genome shuffling to improve yield of GABA, and then the safety of the fusion strain was evaluated. The CLNJ1 was identified as Saccharomyces cerevisiae, and its GABA yield was 1.72 g/L. The GABA yield of CLNJ1-Y breeding by LiCl2 mutagenesis with CLNJ1 was 5.96 g/L, which was 247% as much as CLNJ1. The GABA yield of CLNJ1-YC breeding by genome shuffling with CLNJ1-Y was 12.28 g/L, which was 614% as much as CLNJ1. Hemolysis and antibiotic sensitivity tests were studied for CLNJ1-YC, and the result showed that CLNJ1-YC had no hemolytic activity and was sensitive to the most antibiotics. The S. cerevisiae CLNJ1 can improve the yield of GABA after genome shuffling, and which has good application prospects.

Key words： Saccharomyces cerevisiae CLNJ1; γ-aminobutyric acid; identification; mutagenesis; genome shuffling

Cite this article

CHI Yanping , LU Yawen , WANG Jinghui , LI Da , SU Ying , NIU Honghong , MIAO Xinyu , SUN Mubai , HUA Mei . Identification and genome shuffling breeding of Saccharomyces cerevisiae CLNJ1 producing γ-aminobutyric acid[J]. Food and Fermentation Industries, 2023 , 49(17) : 31 -36 . DOI: 10.13995/j.cnki.11-1802/ts.035749

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