Brassica rapa L. is an edible and medicinal plant that grows on the Tibetan plateau. It has anti-hypoxia and anti-fatigue effects. To study the functional activity and the protective effect on muscle homeostasis of B. rapa extract, its antioxidant activity of it was determined by in vitro free radical scavenging assay first. Then the long-term exercise-induced fatigue mice were used, and the blank group, exercise group, and low and high-dose groups were set up respectively. Firstly, the protective effect on muscle injury was determined by histological observation. Then, the related biomarkers assays and real-time fluorescence quantitative PCR experiments were utilized to analyze the skeletal muscle. It was found that the B. rapa extract could increase the NAD+/NADH ratio and promote ATP synthesis. It also could modulate the SIRT1/PGC-1α/TFAM pathway to enhance mitochondrial function. Then, the Nrf2/HO-1 pathway was regulated and the anti-oxidative stress defense system was enhanced. Finally, it could alleviate muscle injury and promote muscle regeneration by regulating the acta1 and titin-related genes. In conclusion, B. rapa could enhance energy metabolism, maintain the stability of mitochondrial function, improve the ability of antioxidant defense, relieve muscle injury, and promote muscle regeneration, thus playing a role in protecting muscle homeostasis.
WANG Cheng
,
YU Zhongru
,
LI Anyi
,
ZHU Hongkang
,
GUO Yahui
,
CHENG Yuliang
,
QIAN He
. Protective effect of Brassica rapa L. extract on skeletal muscle homeostasis in long-term exercise-induced fatigue mice[J]. Food and Fermentation Industries, 2023
, 49(17)
: 44
-52
.
DOI: 10.13995/j.cnki.11-1802/ts.035969
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