Contamination status and pathogenicity control of Listeria monocytogenes in pork products

  • ZHANG Wenmin ,
  • DONG Qingli ,
  • XIN Bao ,
  • QIAN Wenwen ,
  • REN Xiaomei
Expand
  • 1(School of Public Health, Shaanxi University of Chinese Medicine, Xianyang 712046, China)
    2(School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

Received date: 2022-10-06

  Revised date: 2022-10-17

  Online published: 2023-09-27

Abstract

In recent years, food safety problems caused by the contamination of pork products with Listeria monocytogenes have occurred frequently, which poses a serious threat to public health. The virulence of L. monocytogenes in pork products should be controlled to curb the occurrence of social public health problem. First, this paper summarizes the pollution status of L. monocytogenes in pork in recent years. Then, the pathogenicity of L. monocytogenes is reviewed subsequently, and analyzed the feasibility of controlling the pathogenicity of L. monocytogenes by intervened the growth, resistance and virulence of L. monocytogenes. Finally, hurdle technology is proposed to inhibit L. monocytogenes from different aspects and to enhance the microbiological safety of pork.

Cite this article

ZHANG Wenmin , DONG Qingli , XIN Bao , QIAN Wenwen , REN Xiaomei . Contamination status and pathogenicity control of Listeria monocytogenes in pork products[J]. Food and Fermentation Industries, 2023 , 49(17) : 299 -307 . DOI: 10.13995/j.cnki.11-1802/ts.033859

References

[1] MURRAY E G D, WEBB R A, SWANN M B R.A disease of rabbits characterised by a large mononuclear leucocytosis, caused by a hitherto undescribed bacillus Bacterium monocytogenes (n.sp.)[J].The Journal of Pathology and Bacteriology, 1926, 29(4):407-439.
[2] 中华人民共和国国家统计局. 中国统计年鉴[M].北京:中国统计出版社, 2021.
[3] LI H, WANG P F, LAN R T, et al.Risk factors and level of Listeria monocytogenes contamination of raw pork in retail markets in China[J].Frontiers in Microbiology, 2018, 9:1090.
[4] MORALES-PARTERA A M, CARDOSO-TOSET F, LUQUE I, et al.Prevalence and diversity of Salmonella spp., Campylobacter spp., and Listeria monocytogenes in two free-range pig slaughterhouses[J].Food Control, 2018, 92:208-215.
[5] 邱燕, 温贵兰, 张升波.定点屠宰场(点)猪肉中三种主要食源性致病菌污染情况调查[J].中国动物检疫, 2018, 35(8):23-28.
QIU Y, WEN G L, ZHANG S B.Investigation on pork contamination of three kinds of main food-borne pathogens in pork of fixed-point slaughterhouses[J].China Animal Health Inspection, 2018, 35(8):23-28.
[6] WANG K, YE K P, ZHU Y P, et al.Prevalence, antimicrobial resistance and genetic diversity of Listeria monocytogenes isolated from chilled pork in Nanjing, China[J].LWT - Food Science and Technology, 2015, 64(2):905-910.
[7] EFSA.The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013[J].EFSA Journal, 2015, 13(1):3991.
[8] KANUGANTI S R, WESLEY I V, REDDY P G, et al.Detection of Listeria monocytogenes in pigs and pork[J].Journal of Food Protection, 2002, 65(9):1470-1474.
[9] YU T, JIANG X J.Prevalence and characterization of Listeria monocytogenes isolated from retail food in Henan, China[J].Food Control, 2014, 37:228-231.
[10] WU S, WU Q P, ZHANG J M, et al.Listeria monocytogenes prevalence and characteristics in retail raw foods in China[J].PLoS One, 2015, 10(8):e0136682.
[11] 吴斌, 胡元玮, 方琼楼, 等.金华市生肉食品中致病菌污染状况分析[J].中国卫生检验杂志, 2017, 27(13):1931-1933.
WU B, HU Y W, FANG Q L, et al.Analysis of the contamination by pathogenic bacteria in raw meat in Jinhua[J].China Industrial Economics, 2017, 27(13):1931-1933.
[12] LUO L J, ZHANG Z D, WANG H, et al.A 12-month longitudinal study of Listeria monocytogenes contamination and persistence in pork retail markets in China[J].Food Control, 2017, 76:66-73.
[13] 钱静. 安徽省食品中食源性致病菌监测结果研究[D].合肥:安徽医科大学, 2015.
QIAN J.Study on monitoring results of food-borne pathogens in Anhui Province[D].Hefei:Anhui Medical University, 2015.
[14] 权玉玲, 苏诚玉, 张元国, 等.甘肃省部分地区各类食品中单增李斯特菌污染情况调查分析[J].中国卫生检验杂志, 2009, 19(9):2150-2151.
QUAN Y L, SU C Y, ZHANG Y G, et al.Analysis on Listeria monocytogenes contamination status for most kinds of food in part re-gions of Gansu[J].China Industrial Economics, 2009, 19(9):2150-2151.
[15] 谭海芳, 丁丽娜.2004—2007年肇庆市食品中食源性致病菌监测与分析[J].中国食品卫生杂志, 2009, 21(3):267-270.
TAN H F, DING L N.Surveillance on food-borne pathogens in food in Zhaoqing city from 2004 to 2007[J].Chinese Journal of Food Hygiene, 2009, 21(3):267-270.
[16] 郑秀清, 刘小娟, 陈克功, 等.邯郸市食品中单核细胞增生李斯特菌污染状况调查[J].职业与健康, 2010, 26(12):1359-1360.
ZHENG X Q, LIU X J, CHEN K G, et al.Investigation on food contamination by Listeria monocytogenes in Handan city[J].Occupation and Health, 2010, 26(12):1359-1360.
[17] 张春艳, 阎学燕, 许姣, 等.开封市市售生肉与生肉制品致病菌污染状况分析[J].河南预防医学杂志, 2016, 27(12):955-958.
ZHANG C Y, YAN X Y, XU J, et al. Analysis on contamination by pathogenic bacteria in underessed meay and its processing in Kaifeng[J]. Henan Journal of Preventive Medicine, 2016, 27(12): 955-958.
[18] 郭芳, 黄晓敏, 彭斌, 等.湖北省随州市2009年-2011年食源性致病菌污染状况分析[J].中国卫生检验杂志, 2012, 22(11):2717-2719.
GUO F, HUANG X M, PENG B, et al.Analysis of contamination status of food-borne pathogens in Suizhou from 2009 to 2011[J].Chinese Journal of Health Laboratory Technology, 2012, 22(11):2717-2719.
[19] 唐靓, 李跃中, 徐水祥, 等.我国食品食源性致病菌监测结果分析[J].中国卫生检验杂志, 2016, 26(21):3049-3052.
TANG L, LI Y Z, XU S X, et al.Monitoring results and analysis of food borne pathogen in Chinese food from China[J].Chinese Journal of Health Laboratory Technology, 2016, 26(21):3049-3052.
[20] 文涛, 王文思, 孙葳, 等.辽宁省食品中单增李斯特菌监测分析[J].中国公共卫生, 2015, 31(11):1475-1477.
WEN T, WANG W S, SUN W, et al.Contamination of Listeria monocytogenes in foods in Liaoning Province, 2010-2014[J].Chinese Journal of Public Health, 2015, 31(11):1475-1477.
[21] 王安礼, 马国柱, 李雪梅, 等.陕西省市售肉与肉制食品中食源性致病菌污染监测与分析[J].中国卫生检验杂志, 2010, 20(9):2278-2280.
WANG A L, MA G Z, LI X M, et al.Shaanxi commercial meat and meat Food borne pathogens in food of pollution monitoring and analysis[J].Chinese Journal of Health Laboratory Technology, 2010, 20(9):2278-2280.
[22] 王支兰, 陈伟鑫, 陈培超, 等.上海市嘉定区生禽畜肉及相关食品中致病菌污染状况分析[J].上海预防医学, 2014(12):681-682.
WANG Z L, CHEN W X, CHEN P C, et al. Analysis of pathogenic bacteria pollution in raw livestock meat and related foods in Jiading District, Shanghai[J]. Shanghai Journal of Preventive Medicine, 2014, 26(12):681-682.
[23] 杨修军, 赵薇, 刘桂华, 等.2011—2015年吉林省食品中单增李斯特菌的监测数据分析[J].食品安全质量检测学报, 2017, 8(1):105-110.
YANG X J, ZHAO W, LIU G H, et al.Analysis on the monitoring data of Listeria monocytogenes in food of Jilin Province in 2011-2015[J].Journal of Food Safety & Quality, 2017, 8(1):105-110.
[24] ORSI R H, DEN BAKKER H C, WIEDMANN M.Listeria monocytogenes lineages:Genomics, evolution, ecology, and phenotypic characteristics[J].International Journal of Medical Microbiology, 2011, 301(2):79-96.
[25] PIFFARETTI J C, KRESSEBUCH H, AESCHBACHER M, et al.Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease[J].Proceedings of the National Academy of Sciences of the United States of America, 1989, 86(10):3818-3822.
[26] RASMUSSEN O F, BECK T, OLSEN J E, et al. Listeria monocytogees isolates can be classified into two major types according to the sequence of the listeriolysin gene[J]. Infection And Immunity, 1991, 59: 3945-3951.
[27] WARD T J, DUCEY T F, USGAARD T, et al. Multilocus geno-typing assays for single nucleotide polymorphism-based subtyping of Listeria monocytogenes isolates[J]. Applied and Environmental Microbiology, 2008, 74: 7629-7642.
[28] The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017[J]. EFSA Journal, 2017: 16-18.
[29] 孙照琨, 吴璇, 陈蕊, 等.李斯特菌病既往中国文献报告病例分析[J].中国微生态学杂志, 2016, 28(11):1323-1326.
SUN Z K, WU X, CHEN R, et al.Previous cases of Listeria disease reported in China[J].Chinese Journal of Microecology, 2016, 28(11):1323-1326.
[30] 潘伟, 赵呈元.新生儿感染单核细胞增生李斯特菌1例[J].中国感染控制杂志, 2017, 16(8):771-772.
PAN W, ZHAO C.Listeria monocytogenes infection in newborn:One case report[J].Chinese Journal of Infection Control, 2017, 16(8):771-772.
[31] BUCHANAN R L, GORRIS L G M, HAYMAN M M, et al.A review of Listeria monocytogenes:An update on outbreaks, virulence, dose-response, ecology, and risk assessments[J].Food Control, 2017, 75:1-13.
[32] OMORI Y, MIAKE K, NAKAMURA H, et al.Influence of lactic acid and post-treatment recovery time on the heat resistance of Listeria monocytogenes[J].International Journal of Food Microbiology, 2017, 257:10-18.
[33] COLÁS-MEDÀ P, ABADIAS M, OLIVEIRA M, et al.Influence of fruit matrix and storage temperature on the survival of Listeria monocytogenes in a gastrointestinal simulation[J].Food Control, 2017, 73:1045-1052.
[34] BIERNE H, SABET C, PERSONNIC N, et al.Internalins:A complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes[J].Microbes and Infection, 2007, 9(10):1156-1166.
[35] SWINNEN I A M, BERNAERTS K, DENS E J J, et al.Predictive modelling of the microbial lag phase:A review[J].International Journal of Food Microbiology, 2004, 94(2):137-159.
[36] BRACKETT R E, SCHUMAN J D, BALL H R, et al.Thermal inactivation kinetics of Salmonella spp.within intact eggs heated using humidity-controlled air[J].Journal of Food Protection, 2001, 64(7):934-938.
[37] JUNEJA V K, ALTUNTAŞ E G, AYHAN K, et al.Predictive model for the reduction of heat resistance of Listeria monocytogenes in ground beef by the combined effect of sodium chloride and apple polyphenols[J].International Journal of Food Microbiology, 2013, 164(1):54-59.
[38] EARNSHAW R G, APPLEYARD J, HURST R M.Understanding physical inactivation processes:Combined preservation opportunities using heat, ultrasound and pressure[J].International Journal of Food Microbiology, 1995, 28(2):197-219.
[39] SCHÖFFL F, PRÄNDL R, REINDL A.Regulation of the heat-shock response[J].Plant Physiology, 1998, 117(4):1135-1141.
[40] HARTKE A, FRÉRE J, BOUTIBONNES P, et al.Differential induction of the chaperonin GroEL and the co-chaperonin GroES by heat, acid, and UV-irradiation in Lactococcus lactis subsp.lactis[J].Current Microbiology, 1997, 34(1):23-26.
[41] DUSSURGET O, PIZARRO-CERDA J, COSSART P.Molecular determinants of Listeria monocytogenes Virulence[J].Annual Review of Microbiology, 2004, 58:587-610.
[42] DORTU C, HUCH M, HOLZAPFEL W H, et al.Anti-listerial activity of bacteriocin-producing Lactobacillus curvatus CWBI-B28 and Lactobacillus sakei CWBI-B1365 on raw beef and poultry meat[J].Letters in Applied Microbiology, 2008, 47(6):581-586.
[43] SAÁ IBUSQUIZA P, NIEROP GROOT M, DEBÁN-VALLES A, et al.Impact of growth conditions and role of sigB on Listeria monocytogenes fitness in single and mixed biofilms cultured with Lactobacillus plantarum[J].Food Research International, 2015, 71:140-145.
[44] IGLESIAS M B, VIÑAS I, COLÁS-MEDÀ P, et al.Adhesion and invasion of Listeria monocytogenes and interaction with Lactobacillus rhamnosus GG after habituation on fresh-cut pear[J].Journal of Functional Foods, 2017, 34:453-460.
[45] PETTERSEN K S, SKJERDAL T, WASTESON Y, et al.Survival of Listeria monocytogenes during in vitro gastrointestinal digestion after exposure to 5 and 0.5% sodium chloride[J].Food Microbiology, 2019, 77:78-84.
[46] ARYANI D C, DEN BESTEN H M W, HAZELEGER W C, et al.Quantifying variability on thermal resistance of Listeria monocytogenes[J].International Journal of Food Microbiology, 2015, 193:130-138.
[47] PIZARRO-CERDÁ J, KÜHBACHER A, COSSART P.Entry of Listeria monocytogenes in mammalian epithelial cells:An updated view[J].Cold Spring Harbor Perspectives in Medicine, 2012, 2(11):a010009.
[48] COSSART P.Illuminating the landscape of host-pathogen interactions with the bacterium Listeria monocytogenes[J].Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(49):19484-19491.
[49] BAKARDJIEV A I, STACY B A, PORTNOY D A.Growth of Listeria monocytogenes in the Guinea pig placenta and role of cell-to-cell spread in fetal infection[J].The Journal of Infectious Diseases, 2005, 191(11):1889-1897.
[50] ANDERSEN J B, ROLDGAARD B B, CHRISTENSEN B B, et al.Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs[J].BMC Microbiology, 2007, 7(1):55-60.
[51] RODRIGUES I, TRINDADE M A, PALU A F, et al.Modified atmosphere packaging for lamb meat:Evaluation of gas composition in the extension of shelf life and consumer acceptance[J].Journal of Food Science and Technology, 2018, 55(9):3547-3555.
[52] ZENG J M, ZHANG N, HUANG B, et al.Mechanism of azithromycin inhibition of HSL synthesis in Pseudomonas aeruginosa[J].Scientific Reports, 2016, 6:24299.
[53] CASTELLANO P, BELFIORE C, FADDA S, et al.A review of bacteriocinogenic lactic acid bacteria used as bioprotective cultures in fresh meat produced in Argentina[J].Meat Science, 2008, 79(3):483-499.
[54] MELERO B, VINUESA R, DIEZ A M, et al.Application of protective cultures against Listeria monocytogenes and Campylobacter jejuni in chicken products packaged under modified atmosphere[J].Poultry Science, 2013, 92(4):1108-1116.
[55] HOSSAIN M I, SADEKUZZAMAN M, HA S D.Probiotics as potential alternative biocontrol agents in the agriculture and food industries:A review[J].Food Research International, 2017, 100:63-73.
[56] CALO-MATA P, ARLINDO S, BOEHME K, et al.Current applications and future trends of lactic acid bacteria and their bacteriocins for the biopreservation of aquatic food products[J].Food and Bioprocess Technology, 2008, 1(1):43-63.
[57] WORAPRAYOTE W, MALILA Y, SORAPUKDEE S, et al.Bacteriocins from lactic acid bacteria and their applications in meat and meat products[J].Meat Science, 2016, 120:118-132.
[58] IRKIN R, ESMER O K.Control of Listeria monocytogenes in ground chicken breast meat under aerobic, vacuum and modified atmosphere packaging conditions with or without the presence of bay essential oil at 4.DEG.C[J].Food Science and Technology Research, 2010, 16(4):285-290.
[59] CHOULIARA E, KARATAPANIS A, SAVVAIDIS I N, et al.Combined effect of oregano essential oil and modified atmosphere packaging on shelf-life extension of fresh chicken breast meat, stored at 4 ℃[J].Food Microbiology, 2007, 24(6):607-617.
[60] HUGAS M, PAGÉS F, GARRIGA M, et al.Application of the bacteriocinogenicLactobacillus sakei CTC494 to prevent growth of Listeria in fresh and cooked meat products packed with different atmospheres[J].Food Microbiology, 1998, 15(6):639-650.
[61] LÓPEZ-MENDOZA M C, RUIZ P, MATA C M.Combined effects of nisin, lactic acid and modified atmosphere packaging on the survival of Listeria monocytogenes in raw ground pork[J].International Journal of Food Science & Technology, 2007, 42(5):562-566.
Outlines

/

〈 〉