Campylobacter jejuni, an important zoonotic pathogen, can cause bacterial diarrhea in humans. It is urgent to screen phages that can efficiently lyse C. jejuni and enrich the resource library of the bacterial phages due to the increasing drug resistance of C. jejuni. In this study, a phage target C. jejuni was isolated from chicken manure samples and named vB_Cj_QDYZ. Phage vB_Cj_QDYZ had a positive icosahedral head with the diameter of 100.81 nm and a contractile tail with the length of 65.90 nm, belonging to the Caudovirales order, Myoviridae family. Moreover, phage vB_Cj_QDYZ showed good thermal stability (30-60℃) and pH stability (3-12). Its optimal multiplicity of infection was 0.01 and one-step growth curve showed that the latent period was 60 min, the lytic period was 150 min, and the burst size was 45 PFU/cell. The genome of phage vB_Cj_QDYZ was a double-stranded DNA with a total length of 130 627 bp and a G+C content of 26%. The phage genome contained 165 open reading frames (ORFs) and three tRNAs. Comparative genomics showed that phage vB_Cj_QDYZ may be a new member of the genus Fletchervirus from the subfamily Eucampyvirinae. In conclusion, phage vB_Cj_QDYZ is a lytic phage infecting C. jejuni with strong lysis ability, good thermal and pH stability, and has good application prospects and antibacterial potential.
[1] YOUNG K T, DAVIS L M, DIRITA V J.Campylobacter jejuni:Molecular biology and pathogenesis[J].Nature Reviews Microbiology, 2007, 5(9):665-679.
[2] AL HAKEEM W G, FATHIMA S, SHANMUGASUNDARAM R, et al.Campylobacter jejuni in poultry:Pathogenesis and control strategies[J].Microorganisms, 2022, 10(11):2134.
[3] HUMPHREY T, O′BRIEN S, MADSEN M.Campylobacters as zoonotic pathogens:A food production perspective[J].International Journal of Food Microbiology, 2007, 117(3):237-257.
[4] CONNERTON P L, TIMMS A R, CONNERTON I F.Campylobacter bacteriophages and bacteriophage therapy[J].Journal of Applied Microbiology, 2011, 111(2):255-265.
[5] WAGENAAR J A, VAN BERGEN M A P, MUELLER M A, et al.Phage therapy reduces Campylobacter jejuni colonization in broilers[J].Veterinary Microbiology, 2005, 109(3-4):275-283.
[6] SHARMA S, CHATTERJEE S, DATTA S, et al.Bacteriophages and its applications:An overview[J].Folia Microbiologica, 2017, 62(1):17-55.
[7] PÉREZ PULIDO R, GRANDE BURGOS M J, GÁLVEZ A, et al.Application of bacteriophages in post-harvest control of human pathogenic and food spoiling bacteria[J].Critical Reviews in Biotechnology, 2016, 36(5):851-861.
[8] D’ANGELANTONIO D, SCATTOLINI S, BONI A, et al.Bacteriophage therapy to reduce colonization of Campylobacter jejuni in broiler chickens before slaughter[J].Viruses, 2021, 13(8):1428.
[9] RICHARDS P J, CONNERTON P L, CONNERTON I F.Phage biocontrol of Campylobacter jejuni in chickens does not produce collateral effects on the gut microbiota[J].Frontiers in Microbiology, 2019, 10:476.
[10] CARVALHO C M, GANNON B W, HALFHIDE D E, et al.The in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of Campylobacter coli and Campylobacter jejuni in chickens[J].BMC Microbiology, 2010, 10:232.
[11] LOC CARRILLO C, ATTERBURY R J, EL-SHIBINY A, et al.Bacteriophage therapy to reduce Campylobacter jejuni colonization of broiler chickens[J].Applied and Environmental Microbiology, 2005, 71(11):6554-6563.
[12] USHANOV L, LASAREISHVILI B, JANASHIA I, et al.Application of Campylobacter jejuni phages:Challenges and perspectives[J].Animals, 2020, 10(2):279.
[13] 倪娟,潘道东,孙杨赢,等.弯曲菌噬菌体在家禽生产中的防控进展[J].食品安全质量检测学报,2022,13(3):703-709.
Ni J, PAN D D, SUN Y Y, et al.Progress in the prevention and control of Campylobacter phage in poultry production[J].Journal of Food Safety & Quality,2022,13(3):703-709
[14] 英航宁,李凤鸣,李洁等.宿主菌富集空肠弯曲菌噬菌体培养条件的优化[J].微生物学通报,2021,48(9):3176-3183.
YING H N, LI F M, LI J, et al.Optimization of culture conditions for enrichment of Campylobacter jejuni phages by host bacteria[J].Microbiology China[J].2021,48(9):3176-3183.
[15] 宋召军,姜启东,范正杨,等.一株空肠弯曲菌噬菌体的分离及其生物学特性鉴定[J].中国抗生素杂志,2018,43(8):1020-1025.
SONG Z J, JIANG Q D, FAN Z Y, et al.The isolation and characterization of a lytic phage infecting Campylobacter jejuni[J].2018,43(8):1020-1025.
[16] LIU B, ZHENG T, QUAN R, et al.Biological characteristics and genomic analysis of a novel Vibrio parahaemolyticus phage phiTY18 isolated from the coastal water of Xiamen China[J].Frontiers in Cellular and Infection Microbiology, 2022:1610.
[17] WANG L K, ZHANG J Y, LIU X, et al.Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c[J].Virus Research, 2022, 318:198856.
[18] XIANG Y Y, LI W Y, SONG F, et al.Biological characteristics and whole-genome analysis of the Enterococcus faecalis phage PEf771[J].Canadian Journal of Microbiology, 2020, 66(9):505-520.
[19] SØRENSEN M C H, GENCAY Y E, BRØNDSTED L.Methods for initial characterization of Campylobacter jejuni bacteriophages[J]. Campylobacter jejuni:Methods and Protocols, 2017:91-105.
[20] XUAN G H, KONG J N, WANG Y F, et al.Characterization of the newly isolated Pseudomonas phage vB_Pae_LC3I3[J].Virus Research, 2023, 323:198978.
[21] GONZÁLEZ-GÓMEZ J P, LÓPEZ-CUEVAS O, CASTRO-DEL CAMPO N, et al.Genomic and biological characterization of the novel phages vB_VpaP_AL-1 and vB_VpaS_AL-2 infecting Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND)[J].Virus Research, 2022, 312:198719.
[22] HAN K, ZHU Y N, LI F, et al.Genomic analysis of Acinetobacter phage BUCT629, a newly isolated member of the genus Obolenskvirus[J].Archives of Virology, 2022, 167(4):1197-1199.
[23] YUAN Y, XI H Y, DAI J X, et al.The characteristics and genome analysis of the novel Y.pestis phage JC221[J].Virus Research, 2020, 283:197982.
[24] FURUTA M, NASU T, UMEKI K, et al.Characterization and application of lytic bacteriophages against Campylobacter jejuni isolated from poultry in Japan[J].Biocontrol Science, 2017, 22(4):213-221.
[25] ZAMPARA A, AHERN S J, BRIERS Y, et al.Two distinct modes of Lysis regulation in Campylobacter Fletchervirus and Firehammervirus phages[J].Viruses, 2020, 12(11):1247.
