Food and Fermentation Industries >

2023 , Vol. 49 >Issue 18: 157 - 164

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034120

Preparation of GOx@ZIF-7/PDA in aqueous phase and its enzymatic properties

  • LIU Siyuan ,
  • LIU Jingxing ,
  • WANG Zefen ,
  • ZHU Xiaolu ,
  • LI Yanxue ,
  • LAN Xiongdiao ,
  • LIU Pengru ,
  • LAN Ping
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  • 1(School of Chemistry and Chemical Engineering, Guangxi Minzu University, Key Laboratory of Polysaccharide Materials and Modification, New Technology Laboratory of Chemical and Biological Transformation Process, Nanning 530006, China)
    2(School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530000, China)
    3(Institute of Biological Manufacturing Technology Co.Ltd., Guangxi Institute of Industrial Technology, Nanning 530000, China)

Received date: 2022-10-27

  Revised date: 2022-11-22

  Online published: 2023-10-25

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Abstract

Due to the mild preparation conditions and simple operation, in situ embedding strategy was widely used in the preparation of enzyme-metal organic frameworks (enzyme-MOFs) biocomposites. However, only part of MOFs was successfully employed to synthesize enzyme-MOFs through in situ embedding in aqueous phase. In this study, glucose oxidase (GOx) was encapsulated in zeolitic imidazolate framework-7 (ZIF-7) via in situ embedding in aqueous phase for the first time. A hydrophilic polydopamine (PDA) layer was synthesized on the GOx@ZIF-7 to obtained GOx@ZIF-7/PDA. The preparation conditions of GOx@ZIF-7/PDA were optimized by single factor experiments. The optimized immobilization conditions for GOx@ZIF-7/PDA were showed as follows: immobilization pH 7.0, enzyme 5 g/L, Bim 50 mmol/L, molar ratio of Zn2+ to Bim 1∶9, immobilization temperature 35 ℃, immobilization time 40 min, dopamine 2 g/L. The enzyme activity recovery of GOx@ZIF-7/PDA reached up to (43.34±2.00)% under the optimal condition. Compared with free enzyme, GOx@ZIF-7/PDA achieved better thermal stability, pH stability, storage stability and excellent reuse performance. After 10 times of reuse, the catalytic activity of GOx@ZIF-7/PDA still maintained (96.53±2.26)% of its initial activity. These results indicated the strategy of preparing immobilized enzyme GOx@ZIF-7/PDA via in situ embedding in aqueous phase was efficient and feasible, and it was expected to achieve low cost and long-term stable application of enzyme catalysis.

Key words： glucose oxidase; metal-organic frames; enzyme immobilization; zeolitic imidazolate framework-7 (ZIF-7); in situ embedding method; dopamine

Cite this article

LIU Siyuan , LIU Jingxing , WANG Zefen , ZHU Xiaolu , LI Yanxue , LAN Xiongdiao , LIU Pengru , LAN Ping . Preparation of GOx@ZIF-7/PDA in aqueous phase and its enzymatic properties[J]. Food and Fermentation Industries, 2023 , 49(18) : 157 -164 . DOI: 10.13995/j.cnki.11-1802/ts.034120

References

[1] NASIR Z, ALI A, ALAM M F, et al.Immobilization of GOx enzyme on SiO2-coated Ni-Co ferrite nanocomposites as magnetic support and their antimicrobial and photocatalytic activities[J].ACS Omega, 2021, 6(49):33554-33567.
[2] WONG C M, WONG K H, CHEN X D.Glucose oxidase:Natural occurrence, function, properties and industrial applications[J].Applied Microbiology and Biotechnology, 2008, 78(6):927-938.
[3] BANKAR S B, BULE M V, SINGHAL R S, et al.Glucose oxidase—An overview[J].Biotechnology Advances, 2009, 27(4):489-501.
[4] BAUER J A, ZÁMOCKÁ M, MAJTÁN J, et al.Glucose oxidase, an enzyme "Ferrari":Its structure, function, production and properties in the light of various industrial and biotechnological applications[J].Biomolecules, 2022, 12(3):472.
[5] DROUT R J, ROBISON L, FARHA O K.Catalytic applications of enzymes encapsulated in metal-organic frameworks[J].Coordination Chemistry Reviews, 2019, 381:151-160.
[6] HUANG S M, KOU X X, SHEN J, et al.“Armor-plating” enzymes with metal-organic frameworks (MOFs)[J].Angewandte Chemie International Edition, 2020, 59(23):8786-8798.
[7] HAN J, HUANG W R, ZHAO M, et al.A novel enhanced enrichment glucose oxidase@ZIF-8 biomimetic strategy with 3-mercaptophenylboronic acid for highly efficient catalysis of glucose[J].Colloids and Surfaces.B, Biointerfaces, 2021, 208:112034.
[8] SAKALAUSKIENE L, POPOV A, KAUSAITE-MINKSTIMIENE A, et al.The impact of glucose oxidase immobilization on dendritic gold nanostructures on the performance of glucose biosensors[J].Biosensors, 2022, 12(5):320.
[9] WU X L, YANG C, GE J, et al.Polydopamine tethered enzyme/metal-organic framework composites with high stability and reusability[J].Nanoscale, 2015, 7(45):18883-18886.
[10] CUI J D, FENG Y X, LIN T, et al.Mesoporous metal-organic framework with well-defined cruciate flower-like morphology for enzyme immobilization[J].ACS Applied Materials & Interfaces, 2017, 9(12):10587-10594.
[11] PISKLAK T J, MACÍAS M, COUTINHO D H, et al.Hybrid materials for immobilization of MP-11 catalyst[J].Topics in Catalysis, 2006, 38(4):269-278.
[12] SHIH Y H, LO S H, YANG N S, et al.Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis[J].ChemPlusChem, 2012, 77(11):982-986.
[13] DU Y J, JIA X T, ZHONG L, et al.Metal-organic frameworks with different dimensionalities:An ideal host platform for enzyme@MOF composites[J].Coordination Chemistry Reviews, 2022, 454:214327.
[14] HSU P H, CHANG C C, WANG T H, et al.Rapid fabrication of biocomposites by encapsulating enzymes into Zn-MOF-74 via a mild water-based approach[J].ACS Applied Materials & Interfaces, 2021, 13(44):52014-52022.
[15] LIU D M, CHEN J, SHI Y P.Advances on methods and easy separated support materials for enzymes immobilization[J].TrAC Trends in Analytical Chemistry, 2018, 102:332-342.
[16] HU C, BAI Y X, HOU M A, et al.Defect-induced activity enhancement of enzyme-encapsulated metal-organic frameworks revealed in microfluidic gradient mixing synthesis[J].Science Advances, 2020, 6(5):eaax5785.
[17] ZHUANG J, KUO C H, CHOU L Y, et al.Optimized metal-organic-framework nanospheres for drug delivery:Evaluation of small-molecule encapsulation[J].ACS Nano, 2014, 8(3):2812-2819.
[18] GAO Y A, DOHERTY C M, MULET X.A systematic study of the stability of enzyme/zeolitic imidazolate framework-8 composites in various biologically relevant solutions[J].ChemistrySelect, 2020, 5(43):13766-13774.
[19] XIAO T, LIU D X.Progress in the synthesis, properties and applications of ZIF-7 and its derivatives[J].Materials Today Energy, 2019, 14:100357.
[20] ADHIKARI C, DAS A, CHAKRABORTY A.Zeolitic imidazole framework (ZIF) nanospheres for easy encapsulation and controlled release of an anticancer drug doxorubicin under different external stimuli:A way toward smart drug delivery system[J].Molecular Pharmaceutics, 2015, 12(9):3158-3166.
[21] ZHANG X, TIAN L, WU K X, et al.High sensitivity electrochemiluminescence sensor based on the synergy of ZIF-7 and CdTe for determination of glucose[J].Microchemical Journal, 2022, 177:107254.
[22] LI Z X, DING Y, LI S M, et al.Highly active, stable and self-antimicrobial enzyme catalysts prepared by biomimetic mineralization of copper hydroxysulfate[J].Nanoscale, 2016, 8(40):17440-17445.
[23] WAN G Z, MA X H, JIN L, et al.α-glucosidase immobilization on magnetic core-shell metal-organic frameworks for inhibitor screening from traditional Chinese medicines[J].Colloids and Surfaces B:Biointerfaces, 2021, 205:111847.
[24] 梁鑫, 张成楠, 周威, 等.ZIF-8原位自封装固定化脂肪酶的研究[J].食品研究与开发, 2021, 42(5):1-8.
LIANG X, ZHANG C N, ZHOU W, et al.Study on immobilization of lipase by in-situ encapsulation in ZIF-8[J].Food Research and Development, 2021, 42(5):1-8.
[25] ZHAO Z H, LIN T R, LIU W R, et al.Colorimetric detection of blood glucose based on GOx@ZIF-8@Fe-polydopamine cascade reaction[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2019, 219:240-247.
[26] LU Y Q, LI S Y, CHEN F Y, et al.Development of coin-shaped ZIF-7 functionalized superhydrophobic polysulfone composite foams for continuous removal of oily contaminants from water[J].Journal of Hazardous Materials, 2022, 421:126788.
[27] RYDER M R, CIVALLERI B, BENNETT T D, et al.Identifying the role of terahertz vibrations in metal-organic frameworks:From gate-opening phenomenon to shear-driven structural destabilization[J].Physical Review Letters, 2014, 113(21):215502.
[28] WANG X B, ZHAO Y, WU F C, et al.ZIF-7@carbon composites as multifunctional interlayer for rapid and durable Li-S performance[J].Journal of Energy Chemistry, 2021, 57:19-27.
[29] JI Y, GAO W N, ZHANG S L, et al.Confining natural/mimetic enzyme cascade in an amorphous metal-organic framework for the construction of recyclable biomaterials with catalytic activity[J].Langmuir: the ACS Journal of Surfaces and Colloids, 2022, 38(3):927-936.
[30] MOHAMMAD M, RAZMJOU A, LIANG K, et al.Metal-organic-framework-based enzymatic microfluidic biosensor via surface patterning and biomineralization[J].ACS Applied Materials & Interfaces, 2019, 11(2):1807-1820.
[31] KRIKSTOLAITYTE V, KULIESIUS J, RAMANAVICIENE A, et al.Enzymatic polymerization of polythiophene by immobilized glucose oxidase[J].Polymer, 2014, 55(7):1613-1620.
[32] 高霞, 潘会宾, 乔成芳, 等.基于多级孔金属有机骨架构筑HRP固定化酶反应器及其染料降解应用[J].高等学校化学学报, 2020, 41(7):1591-1599.
GAO X, PAN H B, QIAO C F, et al.Construction of HRP immobilized enzyme reactor based on hierarchically porous metal-organic framework and its dye degradation application[J].Chemical Journal of Chinese Universities, 2020, 41(7):1591-1599.
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