L-serine is one of the 30 most important skeletal compounds in chemistry and materials, but the industrial production of L-serine by microbial fermentation has not yet been realized. Aminodeoxychorismate(ADC) synthase is a key enzyme related to the catabolism of L-serine. Previous study revealed that the gene pabAB encoding ADC synthase was mutated in the high-yielding L-serine mutant strain A36, but the effect of mutation of ADC synthase gene on enzyme activity, strain growth, and L-serine production has not been investigated. In this paper, the pK18mobsacB plasmid was used for the side mutation of pabAB (T426I) on the parent strain SSAAI, and the results showed that L-serine production increased by 15.9% while the biomass of the strain with pabAB 426 side mutation, decreased by 29.3% compared with the parent strain SSAAI. The enzyme activity of ADC synthase was also measured, and it was found that the T426I mutation at position 426 of ADC synthase resulted in a decrease in specific enzyme activity. The recombinant strains A36-Pkan and A36-Dap-e were constructed by using promoters of different strengths to regulate the activity of ADC synthase. The specific enzyme activities of the recombinant ADC synthase increased compared with the parent strain, and the biomass increased by 8.3% and 16%, respectively, while the L-serine production decreased by 18.6% and 22.6% compared with the parent strain. This indicates that increasing the enzymatic activity of ADC synthase is beneficial to the growth of Corynebacterium glutamicum but not to the production of L-serine.
LIU Anqian
,
YAN Wenbin
,
XIAO Wenhan ZHANG Xiaomei
,
SHI Jinsong
,
XU Zhenghong
. Effect of aminodeoxychorismate synthase on the cell growth and L-serine production in Corynebacterium glutamicum[J]. Food and Fermentation Industries, 2023
, 49(19)
: 15
-21
.
DOI: 10.13995/j.cnki.11-1802/ts.034927
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