Food and Fermentation Industries >

2023 , Vol. 49 >Issue 19: 67 - 74

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034801

Strain identification of Bifidobacterium longum subsp. infantis YLGB-1496 isolated from breast milk

  • LIU Chong ,
  • MA Xia ,
  • YU Xuejian ,
  • LIU Yiru ,
  • LIU Wei-hsien ,
  • LIU Rui ,
  • XIN Di ,
  • TANG Tengfei ,
  • LIU Hongqiang ,
  • GE Yuanyuan ,
  • SUN Ting ,
  • JIANG Qiuyue ,
  • HUNG Wei-Lian ,
  • YAO Su
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  • 1(China National Research Institute of Food and Fermentation Industries Co.LTD., China Center of Industrial Culture Collection, Beijing 100015, China)
    2(Inner Mongolia Dairy Technology Research Institute Co.Ltd., Hohhot 010110, China)
    3(Inner Mongolia Yili Industrial Group Co.Ltd., Hohhot 010110, China)

Received date: 2023-01-03

  Revised date: 2023-02-07

  Online published: 2023-11-01

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Abstract

Bifidobacterium longum subsp. infantis YLGB-1496 isolated from breast milk was studied in this paper. Five strains of YLGB-1496 from different batches were collected as target strains, and eight strains of B. longum were collected as the references, and other 28 complete genomes of B. longum subsp. infantis were collected as references. Microbial identification technologies including morphology, physiological and biochemical analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and whole genome sequencing were applied to establish the strain-level identification method suitable for the identification of strain YLGB-1496. The results showed that the average nucleotide identity values of five strains of YLGB-1496 were greater than 98%, and the digital DDH values were all greater than 85% comparing with the type strain B. longum subsp. infantis ATCC 15697T. Five strains of YLGB-1496 from different sources were identified as B. longum subsp. infantis. Complete genome multilocus sequence typing (cgMLST) analysis was carried out based on the 1 059 core genes of the strains of YLGB-1496 and other reference strains, the results showed that the strains YLGB-1496 could be effectively distinguished from other reference strains. Single nucleotide polymorphism (SNP) analysis was carried out based on the whole genome sequence of the strain YLGB-1496-1, and results showed that the difference in SNP between different sources of YLGB-1496 was less than 30, and greater than 18 000 between reference strains. Phylogenetic analysis further demonstrated that the SNP analysis can effectively distinguish YLGB-1496 from other reference strains. The safety and function of probiotics were specific at the strain level. The strain-level identification method that combines phenotype and genotype established in this study could provide a technical basis for the establishment of strain identification relative standards and promote the safer and wider application of probiotics in the food industry.

Key words： Bifidobacterium longum subsp. infantis; strain identification; whole genome sequencing; complete genome multilocus sequence typing (cgMLST); single nucleotide polymorphism (SNP)

Cite this article

LIU Chong , MA Xia , YU Xuejian , LIU Yiru , LIU Wei-hsien , LIU Rui , XIN Di , TANG Tengfei , LIU Hongqiang , GE Yuanyuan , SUN Ting , JIANG Qiuyue , HUNG Wei-Lian , YAO Su . Strain identification of Bifidobacterium longum subsp. infantis YLGB-1496 isolated from breast milk[J]. Food and Fermentation Industries, 2023 , 49(19) : 67 -74 . DOI: 10.13995/j.cnki.11-1802/ts.034801

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