5-Hydroxytryptamine (5-HT) is a neurotransmitter produced by enterochromaffin cells (EC) that make up less than 1% of the number of intestinal epithelial cells, tryptophan hydroxylase (TPH1) is the rate-limiting enzyme in 5-HT synthesis pathway. Specific probiotics can regulate the expression of the Tph1 gene in intestinal chromaffin cells to maintain normal intestinal 5-HT synthesis. However, the mechanism of action remains unclear. In this study, metabolomics technology was used to analyze the main active substances in probiotic fermentation products. The interaction dose-effect relationship between the metabolites and the expression of the Tph1 gene was established based on the small intestinal organoids. We identified L-lactic acid and acetic acid as the essential effector substances in regulating intestinal 5-HT synthesis by probiotics. The results of this study clarified the material basis of the physiological function of probiotics and provided theoretical support for the targeted screening of similar functional probiotics.
WANG Zheng
,
TIAN Peijun
,
ZHU Ran
,
WANG Gang
,
CHEN Wei
. Unravelling the effective substances of a probiotic strain regulating 5-HT synthesis in enterochromaffin cells using intestinal organoids[J]. Food and Fermentation Industries, 2023
, 49(19)
: 75
-82
.
DOI: 10.13995/j.cnki.11-1802/ts.034490
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