High-gravity brewing technology is a common production technology in the beer industry. It is of great production significance to breed high-gravity brewing yeast with excellent fermentation performance. A brewer's yeast G03 (Saccharomyces pastorianus) was improved by genome shuffling. Three rounds of screening were carried out to obtain brewer's yeast with efficient fermentation ability. They were Durham's tube gas production test, strain tolerance test, and triangular flask fermentation test. G03 was subjected to atmospheric room temperature plasma mutagenesis and two rounds of recursive protoplast fusion, and finally, the strain F2-123 with increased fermentation rate and fermentation attenuation was obtained, and the content of the main flavor components was not significantly affected. The fermentation performance of F2-123 was stable during the passaged culture. The beer fermentation test of 24 °P wort at 11℃ showed that: the fermentation period of F2-123 was 11 d, which was 1 d shorter than that of the starting strain G03; the alcohol content increased by 8.9%; the real attenuation increased by 6.5%, and there was no significant change in other flavor components. The strain of this study has some potential for application in actual beer production.
ZHANG Yameng
,
WANG Jinjing
,
NIU Chengtuo
,
ZHENG Feiyun
,
LIU Chunfeng
,
LI Qi
. Breeding brewing yeast with improved fermentation performance in high-gravity beer fermentation by genome shuffling[J]. Food and Fermentation Industries, 2023
, 49(19)
: 97
-104
.
DOI: 10.13995/j.cnki.11-1802/ts.034864
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