Food and Fermentation Industries >

2023 , Vol. 49 >Issue 19: 160 - 167

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032623

Redictionlocalization and identification of linear epitopes ofegg allergen Gal d 4

  • WANG Tao ,
  • TANG Xinlei ,
  • LI Qian ,
  • JIANG Songsong
Expand
  • (College of Tourism and Culinary, Yangzhou University, Yangzhou 225127, China)

Received date: 2022-06-09

  Revised date: 2022-09-20

  Online published: 2023-11-01

Fold

Abstract

This study aimed to analyze the primary amino acid sequence, secondary structure to predict the B cell linear epitopes and T cell epitopes of Gal d 4 by bioinformatics software (GenBank database, PDB database, Immunomedicine Group, Bepipred-2.0 Server, ABCpred, NetMHCⅡpan 4.0 Serer, SYFPEITHI, NetMHC, and NetCTLonline tools), and the study also positioned the linear epitopes on the spatial model of lysozyme tertiary structure. The linear B cell epitopes of Gal d 4 were identified by serum of patients. esults showed that the same B cell linear epitopes predicted by the three bioinformatics software were 19KVFGRCELAAAMKR32,57NTQA60,65TDG67,87TPGSRNLCNI96, and 132RCKGTDVQAWI142. In addition, 19KVFGRCELAAAMKR32,57NTQA60 and 132RCKGTDVQAWI142,65TDG67 and 87TPGSRNLCNI96 were discontinuous in amino acid sequence, but adjacent in spatial structure, which made them have the potential to form conformational epitopes. The predicted T cell epitopes were 42SLGNWVCAA50 and 100ALLSSDITA108. The linear B cell epitope immunoreactivity was identified as 19KVFGRCELAAAMKR32. This study was the first to predict the linear B cell epitopes and T cell epitopes of Gal d 4. The prediction of linear epitopes of Gal d 4 will be helpful to further understand the function and structure of Gal d 4 and will provide important reference for the identification and detection of egg allergens.

Key words： egg allergen; lysozyme; bioinformatics; B cell linear epitopes; T cell epitopes

Cite this article

WANG Tao , TANG Xinlei , LI Qian , JIANG Songsong . Redictionlocalization and identification of linear epitopes ofegg allergen Gal d 4[J]. Food and Fermentation Industries, 2023 , 49(19) : 160 -167 . DOI: 10.13995/j.cnki.11-1802/ts.032623

References

[1] MURARO A, LEMANSKE R F Jr, CASTELLS M, et al.Precision medicine in allergic disease-food allergy, drug allergy, and anaphylaxis-PRACTALL document of the European Academy of Allergy and Clinical Immunology and the American Academy of Allergy, Asthma and Immunology[J].Allergy, 2017, 72(7):1006-1021.
[2] DONA D W, SUPHIOGLU C.Egg allergy:Diagnosis and immunotherapy[J].International Journal of Molecular Sciences, 2020, 21(14):5010.
[3] LOH W, TANG M L K.The epidemiology of food allergy in the global context[J].International Journal of Environmental Research and Public Health, 2018, 15(9):2043.
[4] 陈寅. 牛奶、鸡蛋过敏原的鉴定、纯化及应用研究[D].天津:天津医科大学, 2011.
CHEN Y.Identification, purification and application research of allergens from milk and hen's egg[D].Tianjin:Tianjin Medical University, 2011.
[5] MARTORELL A, ALONSO E, BONÉ J, et al.Position document:IgE-mediated allergy to egg protein[J].Allergologia et Immunopathologia, 2013, 41(5):320-336.
[6] MARTOS G, LÓPEZ-FANDIÑO R, MOLINA E.Immunoreactivity of hen egg allergens:Influence on in vitro gastrointestinal digestion of the presence of other egg white proteins and of egg yolk[J].Food Chemistry, 2013, 136(2):775-781.
[7] INFANTE S, LÓPEZ-MATAS M Á, CARNÉS J, et al.Allergy reaction mediated by Gal d 4 (lysozyme) after the induction of tolerance with egg[J].Annals of Allergy, Asthma & Immunology, 2014, 113(4):491-492.
[8] ELBANY C, DE BOISSIEU D, KARILA C, et al.Unusual Lysozyme-induced anaphylaxis in an egg-allergic child[J].Authorea Preprints, 2021.DOI:10.22541/au.163664664.49008784/v1
[9] LIU C Q, SATHE S K.Food allergen epitope mapping[J].Journal of Agricultural and Food Chemistry, 2018, 66(28):7238-7248.
[10] PALOMARES O, AKDIS M, MARTÍN-FONTECHA M, et al.Mechanisms of immune regulation in allergic diseases:The role of regulatory T and B cells[J].Immunological Reviews, 2017, 278(1):219-236.
[11] 李欣, 马鑫, 谭宏凯, 等.T细胞及其表位在食物过敏中的研究进展[J].食品与生物技术学报, 2020, 39(10):18-25.
LI X, MA X, TAN H K, et al.Research progress in T cells and their epitopes in food allergy[J].Journal of Food Science and Biotechnology, 2020, 39(10):18-25.
[12] 刘阳星月, 张迪, 姚亚亚, 等.大豆过敏原11S球蛋白G2中A2链结合表位的预测[J].食品科学, 2018, 39(18):152-158.
LIU Y, ZHANG D, YAO Y Y, et al.Mapping of ig G-binding epitopes on the A2 chain of the major soybean allergen 11S globulin G2[J].Food Science, 2018, 39(18):152-158.
[13] EL-MANZALAWY Y, HONAVAR V.Recent advances in B-cell epitope prediction methods[J].Immunome Research, 2010, 6(2):S2.
[14] SUN P P, JU H X, LIU Z B, et al.Bioinformatics resources and tools for conformational B-cell epitope prediction[J].Computational and Mathematical Methods in Medicine, 2013, 2013:943636.
[15] RUBINSTEIN N D, MAYROSE I, HALPERIN D, et al.Computational characterization of B-cell epitopes[J].Molecular Immunology, 2008, 45(12):3477-3489.
[16] RAHIMPOUR E, SAMAD-SOLTANI T.Immunoinformatics:A basic bibliometric analysis[J].ImmunoAnalysis, 2021, 1(1):2.
[17] HOPP T P, WOODS K R.Prediction of protein antigenic determinants from amino acid sequences[J].Proceedings of the National Academy of Sciences of the United States of America, 1981, 78(6):3824-3828.
[18] EMINI E A, HUGHES J V, PERLOW D S, et al.Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide[J].Journal of Virology, 1985, 55(3):836-839.
[19] KARPLUS P A, SCHULZ G E.Prediction of chain flexibility in proteins[J].Naturwissenschaften, 1985, 72(4):212-213.
[20] JAMESON B A, WOLF H.The antigenic index:A novel algorithm for predicting antigenic determinants[J].Bioinformatics, 1988, 4(1):181-186.
[21] FU L L, WANG J B, NI S Q, et al.Identification of allergenic epitopes and critical amino acids of major allergens in Chinese shrimp (Penaeus chinensis) by immunoinformatics coupled with competitive-binding strategy[J].Journal of Agricultural and Food Chemistry, 2018, 66(11):2944-2953.
[22] POSCH A, WEISS W, WHEELER C, et al.Sequence analysis of wheat grain allergens separated by two-dimensional electrophoresis with immobilized pH gradients[J].Electrophoresis, 1995, 16(1):1115-1119.
[23] LI J Y, BAI X J, LIANG Y, et al.Prediction of epitopes of Rv1410c Mycobacterium tuberculosis protein using DNAStar software[J].Chinese Journal of Cellular and Molecular Immunology, 2015, 31(4):474-477.
[24] KOLASKAR A S, TONGAONKAR P C.A semi-empirical method for prediction of antigenic determinants on protein antigens[J].FEBS Letters, 1990, 276(1-2):172-174.
[25] JESPERSEN M C, PETERS B, NIELSEN M, et al.BepiPred-2.0:Improving sequence-based B-cell epitope prediction using conformational epitopes[J].Nucleic Acids Research, 2017, 45(W1):W24-W29.
[26] ISEA R.Quantitative prediction of linear B-cell epitopes[EB/OL].2017:arXiv:1703.02453.https://arxiv.org/abs/1703.02453
[27] GORGAN L, PETRESCU-MAG V.The small heat shock proteins structure in Carassius gibelio and Oncorhynchus mykiss[J].Journal of Experimental and Molecular Biology, 2009, 10(4):19-26.
[28] 龙伟, 李欣, 高金燕, 等.生物信息学技术在食物过敏原表位预测中的应用[J].食品科学, 2014, 35(3):259-263.
LONG W, LI X, GAO J Y, et al.Application of bioinformatics technology in prediction of food allergen epitopes[J].Food Science, 2014, 35(3):259-263.
[29] LI X, CHEN H B, TONG P, et al.Epitope mapping of buffalo beta-lactoglobulin against rabbit polyclonal antibody following phage display technique[J].Journal of Food Biochemistry, 2012, 36(1):56-65.
[30] KARIMI M, IGNASIAK M T, CHAN B, et al.Reactivity of disulfide bonds is markedly affected by structure and environment:Implications for protein modification and stability[J].Scientific Reports, 2016, 6:38572.
[31] SHADDEL M, EBRAHIMI M, TABANDEH M R.Bioinformatics analysis of single and multi-hybrid epitopes of GRA-1, GRA-4, GRA-6 and GRA-7 proteins to improve DNA vaccine design against Toxoplasma gondii[J].Journal of Parasitic Diseases, 2018, 42(2):269-276.
[32] 马秀丽, 张九凯, 孙劲旅, 等.芝麻过敏原Ses i 3同源建模及B细胞线性抗原表位预测[J].中国食品学报, 2021, 21(4):9-18.
MA X L, ZHANG J K, SUN J L, et al.Homology modeling and prediction on B cell liner antigenic epitopes of Ses i 3 from Sesamum indicum[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(4):9-18.
[33] 李雪娇, 李欣芮, 范卓妍, 等.小麦主要过敏原CM16线性B细胞表位的预测及初步鉴定[J].食品科学, 2019, 40(24):136-141.
LI X J, LI X R, FAN Z Y, et al.Predicting and identifying linear B-cell epitopes of wheat allergen CM16[J].Food Science, 2019, 40(24):136-141.
[34] GUO X Y, JIANG S S, LI X R, et al.Sequence analysis of digestion-resistant peptides may be an efficient strategy for studying the linear epitopes of Jug r 1, the major walnut allergen[J].Food Chemistry, 2020, 322:126711.
[35] CREECH A L, TING Y S, GOULDING S P, et al.The role of mass spectrometry and proteogenomics in the advancement of HLA epitope prediction[J].PROTEOMICS, 2018, 18(12):1700259.
[36] PASCAL M, KONSTANTINOU G N, MASILAMANI M, et al.In silico prediction of Ara h 2 T cell epitopes in peanut-allergic children[J].Clinical & Experimental Allergy, 2013, 43(1):116-127.
[37] 张春华. 基于信息融合和计算智能的构象性B细胞表位预测方法研究[D].长春:东北师范大学, 2016.
ZHANG C H.The research of conformational B-cell epitope prediction method based on information fusion and computational intelligence[D].Changchun:Northeast Normal University, 2016.
[38] GOEDHALS D, PAWESKA J T, BURT F J.Identification of human linear B-cell epitope sites on the envelope glycoproteins of Crimean-Congo haemorrhagic fever virus[J].Epidemiology and Infection, 2015, 143(7):1451-1456.
[39] GEYSEN H M, RODDA S J, MASON T J, et al.Strategies for epitope analysis using peptide synthesis[J].Journal of Immunological Methods, 1987, 102(2):259-274.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.