Food and Fermentation Industries >

2023 , Vol. 49 >Issue 20: 17 - 22

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034124

Metabolomic study of methylated transferase mutants of Lacticaseibacillus paracasei in response to osmotic stress

  • WU Meiling ,
  • ZHANG Wenyi
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  • 1(Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China)
    2(Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China)

Received date: 2022-10-27

  Revised date: 2022-12-20

  Online published: 2023-11-20

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Abstract

DNA methylation is an important epigenetic regulatory mechanism, which is closely related to many physiological functions of bacteria. In this study, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry was used to compare and analyze the Lacticaseibacillus paracasei Zhang methyltransferase mutant strain and its wild-type metabolome in an osmotic stress environment, and explore its significantly different metabolites. Through multivariate statistical analysis including principal component analysis and orthogonal partial least squares discriminant analysis, combined with database search and mass spectrometry information matching, a total of 10 significantly different metabolites were screened (P<0.05). These metabolites mainly include cortisol, 18-hydroxycorticosterone, deoxyinosine, betaine aldehyde, and glycochenodeoxycholic acid, which were involved in multiple metabolic pathways such as steroid hormone biosynthesis, purine metabolism, glycine, serine, and threonine metabolism, and primary bile acid metabolism. These results help to reveal the molecular mechanism of DNA methylation mediating the adaptation of L. paracasei Zhang to osmotic stress environment.

Key words： Lacticaseibacillus paracasei Zhang; DNA methylation; osmotic stress; metabolomics

Cite this article

WU Meiling , ZHANG Wenyi . Metabolomic study of methylated transferase mutants of Lacticaseibacillus paracasei in response to osmotic stress[J]. Food and Fermentation Industries, 2023 , 49(20) : 17 -22 . DOI: 10.13995/j.cnki.11-1802/ts.034124

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