D-allulose has been a hot research topic in recent years, which is a food additive with multiple physiological functions. To investigate the bioconversion of D-allulose, three different microbial sources of D-allulose 3-epimerases (DAEase) were selected for heterologous expression in E. coli BL21(DE3). Through the exploration of the whole-cell bioconversion and the analysis of the catalytic capacity of different recombinant strains, it was concluded that DAEase from Dorea sp. CAG317 was shown to have a more promising industrial potential with a conversion rate of 32.60%. Based on this, we carried out chromatographic separation of the reaction solution to obtain a high-purity D-allulose stock solution. We took the cooling crystallization method for D-allulose stock solution to precipitate crystals, and the results showed that the optimized cooling crystallization processes achieved a 77.43% crystallization rate and a 98.70% purity of the product. This study provides an important reference for the upstream and downstream processes for the industrial production of D-allulose.
SUN Zhen
,
CHENG Qianqian
,
JI Qinyi
,
ZHU Mulan
,
XIA Yu
. Optimization of bioconversion and cooling crystallization processes for D-allulose[J]. Food and Fermentation Industries, 2023
, 49(21)
: 16
-22
.
DOI: 10.13995/j.cnki.11-1802/ts.034200
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