Food and Fermentation Industries >

2023 , Vol. 49 >Issue 22: 27 - 32

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034793

Biological activity of exopolysaccharides from Pediococcus acidilactici HAO2018

  • JIANG Jiayun ,
  • ZHAO Chongjie ,
  • LI Xuening ,
  • HE Hongpeng ,
  • LI Junxun ,
  • ZHANG Xiaoyuan ,
  • CHEN Mian ,
  • LIU Fei ,
  • WANG Nan
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  • 1(School of Bioengineering, Tianjin University of Science and Technology, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China)
    2(Shandong Taishan Shengliyuan Group Co. Ltd., Taian 271000, China)
    3(Shandong Academy of Pharmaceutical Sciences, Jinan 250101, China)

Received date: 2023-01-03

  Revised date: 2023-02-09

  Online published: 2023-12-25

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Abstract

A strain of Pediococcus acidilactici HAO2018 isolated from fermented grains of Maotai-flavor Baijiu was taken as the research object to explore its acid tolerance, bile salt tolerance, and drug resistance. Exopolysaccharides (EPS) of this strain were extracted by ethanol precipitation method, and their biological activities such as anti-oxidation, anti-bacterial and anti-tumor were evaluated. Results showed that P. acidilactici HAO2018 had good acid and bile salt tolerance, was sensitive to multiple antibiotics such as kanamycin, etc., and was not sensitive to vancomycin and cotrimoxazole. The DPPH free radical, hydroxyl radical scavenging rates and reducing ability of EPS increased in a concentration-dependent manner. When the concentration of EPS was 1.0 mg/mL, the DPPH scavenging rate was (68.46±4.16)%. When the concentration of EPS was 10.0 mg/mL, the reducing ability and hydroxyl radical scavenging rate were (54.10±0.15)% and (83.43±3.87)%, respectively. The EPS of the strain HAO2018 had a significant inhibitory effect on food borne pathogenic bacteria Shigella and Cronobacter sakazakii. The anti-tumor activity of EPS was concentration-dependent. 0.5-2 mg/mL EPS treatment significantly inhibited the proliferation of HT-29 cells at 48 h and 72 h. These studies provide a theoretical basis for the comprehensive development and utilization of the exopolysaccharides of Pediococcus acidilactici.

Key words： Pediococcus acidilactici; exopolysaccharides; antioxidant activity; bacteriostatic activity; antitumor activity

Cite this article

JIANG Jiayun , ZHAO Chongjie , LI Xuening , HE Hongpeng , LI Junxun , ZHANG Xiaoyuan , CHEN Mian , LIU Fei , WANG Nan . Biological activity of exopolysaccharides from Pediococcus acidilactici HAO2018[J]. Food and Fermentation Industries, 2023 , 49(22) : 27 -32 . DOI: 10.13995/j.cnki.11-1802/ts.034793

References

[1] SHENG S X, FU Y B, PAN N, et al.Novel exopolysaccharide derived from probiotic Lactobacillus pantheris TCP102 strain with immune-enhancing and anticancer activities[J].Frontiers in Microbiology, 2022, 13:1015270.
[2] STAGE M, WICHMANN A, JØRGENSEN M, et al.Lactobacillus rhamnosus GG genomic and phenotypic stability in an industrial production process[J].Applied and Environmental Microbiology, 2020, 86(6): e02780-19.
[3] ZHOU X T, QI W C, HONG T, et al.Exopolysaccharides from Lactobacillus plantarum NCU116 regulate intestinal epithelial barrier function via STAT3 signaling pathway[J].Journal of Agricultural and Food Chemistry, 2018, 66(37):9719-9727.
[4] LI S J, CHEN T T, XU F, et al.The beneficial effect of exopolysaccharides from Bifidobacterium bifidum WBIN03 on microbial diversity in mouse intestine[J].Journal of the Science of Food and Agriculture, 2014, 94(2):256-264.
[5] AI L Z, ZHANG H, GUO B H, et al.Preparation, partial characterization and bioactivity of exopolysaccharides from Lactobacillus casei LC2 W[J].Carbohydrate Polymers, 2008, 74(3):353-357.
[6] DILNA S V, SURYA H, ASWATHY R G, et al.Characterization of an exopolysaccharide with potential health-benefit properties from a probiotic Lactobacillus plantarum RJF 4[J].LWT-Food Science and Technology, 2015, 64(2):1179-1186.
[7] LIU C F, TSENG K C, CHIANG S S, et al.Immunomodulatory and antioxidant potential of Lactobacillus exopolysaccharides[J].Journal of the Science of Food and Agriculture, 2011, 91(12):2284-2291.
[8] DI W, ZHANG L W, YI H X, et al.Exopolysaccharides produced by Lactobacillus strains suppress HT-29 cell growth via induction of G0/G1 cell cycle arrest and apoptosis[J].Oncology Letters, 2018, 16(3):3577-3586.
[9] LI B L, DU P, SMITH E E, et al.In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress[J].Food & Function, 2019, 10(3):1707-1717.
[10] XIAO L Y, GE X J, YANG L, et al.Anticancer potential of an exopolysaccharide from Lactobacillus helveticus MB2-1 on human colon cancer HT-29 cells via apoptosis induction[J].Food & Function, 2020, 11(11):10170-10181.
[11] RIAZ RAJOKA M S, JIN M L, ZHAO H B, et al.Functional characterization and biotechnological potential of exopolysaccharide produced by Lactobacillus rhamnosus strains isolated from human breast milk[J].LWT, 2018, 89:638-647.
[12] 何红鹏, 张同存, 王楠, 等.一种具有抗宫颈癌作用的乳酸片球菌及应用:中国, CN111849805B[P].2022-04-22.
HE H P, ZHANG T C, WANG N, et al.A kind of Lactobacillus with anti-cervical cancer effect and its application:China, CN111849805B[P].2022-04-22.
[13] DE OLIVEIRA COELHO B, FIORDA-MELLO F, DE MELO PEREIRA G, et al.In vitro probiotic properties and DNA protection activity of yeast and lactic acid bacteria isolated from a honey-based kefir beverage[J].Foods, 2019, 8(10):485.
[14] GAO X J, WEI J H, HAO T Y, et al.Dysgonomonas mossii strain ShenZhen WH 0221, a new member of the genus dysgonomonas isolated from the blood of a patient with diabetic nephropathy, exhibits multiple antibiotic resistance[J].Microbiology Spectrum, 2022, 10(4):e02381-21.
[15] TAYLAN O, YILMAZ M T, DERTLI E.Partial characterization of a levan type exopolysaccharide (EPS) produced by leuconostoc mesenteroides showing immunostimulatory and antioxidant activities[J].International Journal of Biological Macromolecules, 2019, 136:436-444.
[16] TABIBZADEH F, ALVANDI H, HATAMIAN-ZARMI A, et al.Antioxidant activity and cytotoxicity of exopolysaccharide from mushroom Hericium coralloides in submerged fermentation[J].Biomass Conversion and Biorefinery, 2022:1-11.
[17] LIU Y W, GLOAG E S, HILL P J, et al.Interbacterial antagonism mediated by a released polysaccharide[J].Journal of Bacteriology, 2022, 204(5):e00076-22.
[18] SUN N X, LIU H P, LIU S J, et al.Purification, preliminary structure and antitumor activity of exopolysaccharide produced by Streptococcus thermophilus CH9[J].Molecules, 2018, 23(11):2898.
[19] 李尧, 卢承蓉, 刘丹, 等.乳酸片球菌胞外多糖的分离纯化、结构分析及抗氧化活性研究[J].食品与发酵工业, 2021, 47(19):35-42.
LI Y, LU C R, LIU D, et al.Structure and antioxidant activity of Pediococcus lactis extracellular polysaccharide[J].Food and Fermentation Industries, 2021, 47(19):35-42.
[20] SONG Y R.Exopolysaccharide produced by Pediococcus acidilactici M76 isolated from the Korean traditional rice wine, Makgeolli[J].Journal of Microbiology and Biotechnology, 2013, 23(5):681-688.
[21] KUMAR R, BANSAL P, SINGH J, et al.Purification partial structural characterization and health benefits of exopolysaccharides from potential probiotic Pediococcus acidilactici NCDC 252[J].Process Biochemistry, 2020, 99:79-86.
[22] 方伟, 李佳佳, 耿伟涛, 等.发酵乳杆菌CECT 5716产胞外多糖培养基成分优化及抗氧化活性研究[J].中国酿造, 2022, 41(11):187-192.
FANG W, LI J J, GENG W T, et al.Optimization for medium components of exopolysaccharide production by Lactobacillus fermentum CECT 5716 and antioxidant activity[J].China Brewing, 2022, 41(11):187-192.
[23] 谢莹, 蔡国林, 刘逸凡, 等.双歧杆菌BB12胞外多糖发酵条件优化及抗氧化活性研究[J].食品与发酵工业, 2019, 45(23):55-59.
XIE Y, CAI G L, LIU Y F, et al.Optimization of fermentation conditions and antioxidant activities of exopolysaccharide from Bifidobacterium lactis Bb12[J].Food and Fermentation Industries, 2019, 45(23):55-59.
[24] 丁涛, 靳宝林, 杨晓宇, 等.一株高产胞外多糖的乳酸芽孢杆菌的筛选及功能鉴定[J].四川大学学报(自然科学版), 2021, 58(6):159-166.
DING T, JIN B L, YANG X Y, et al.Screening and functional identification of a Lactobacillus strain with high exopolysaccharide production[J].Journal of Sichuan University (Natural Science Edition), 2021, 58(6):159-166.
[25] RANI R P, ANANDHARAJ M, DAVID RAVINDRAN A.Characterization of a novel exopolysaccharide produced by Lactobacillus gasseri FR4 and demonstration of its in vitro biological properties[J].International Journal of Biological Macromolecules, 2018, 109:772-783.
[26] SUN M Y, LIU W W, SONG Y L, et al.The effects of Lactobacillus plantarum-12 crude exopolysaccharides on the cell proliferation and apoptosis of human colon cancer (HT-29) cells[J].Probiotics and Antimicrobial Proteins, 2021, 13(2):413-421.
[27] SONG W, HU P, GUO S L, et al.Oxidative stress and endoplasmic reticulum stress contribute to L.paracasei subsp.paracasei M5L exopolysaccharide-induced apoptosis in HT-29 cells[J].Food Science & Nutrition, 2021, 9(3):1676-1687.
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