Food and Fermentation Industries >

2023 , Vol. 49 >Issue 22: 49 - 56

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033101

The immunomodulatory effects of oyster peptide on mouse RAW264.7 cells

  • LI Jinhong ,
  • ZHENG Huizhen ,
  • CHEN Hui ,
  • LIU Shulai ,
  • GU Saiqi ,
  • WANG Rui ,
  • XIANG Xingwei
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  • 1(College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China)
    2(Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Hangzhou 310014, China)
    3(National R&D Branch Center for Pelagic Aquatic Products Processing, Hangzhou 310014, China)
    4(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University,Dalian 116034, China)

Received date: 2022-07-26

  Revised date: 2022-09-06

  Online published: 2023-12-25

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Abstract

To study the effect of oyster peptides on the immune function of mouse macrophage RAW264.7 and its mechanism, the effects of different concentrations of oyster peptides on the proliferation of RAW264.7 macrophages were investigated by cell counting kit-8 in this study. The phagocytosis of macrophages, secretion of NO, TNF-α, IL-1β, IL-6, and IL-10 and the relative mRNA expression levels of these cytokines were detected by neutral red method, nitrate reductase method, enzyme-linked immunosorbent assay, and real-time fluorescence quantitative PCR. The expression of protein p-p65/p65 and p-IκBα/IκBα in the NF-κB pathway of macrophages was detected by Western-blot. Results showed that OP concentration within 1 000 μg/mL had no significant effect on cell proliferation (P>0.05). OP could also enhance the phagocytosis of macrophages, and significantly increase the secretion of NO, TNF-α, IL-1β, IL-6 and IL-10 and the relative mRNA expression levels in RAW264.7 cells (P<0.05), while the regulating effect was dose-dependent. Western-blot results showed that OP significantly decreased the levels of IκBα and p65 phosphorylation in RAW264.7 cells compared with the lipopolysaccharide-induced group (P<0.05). These results suggested that OP had an immunomodulatory effect on mouse macrophage RAW264.7, and the mechanism may be related to the activation of the NF-κB pathway.

Key words： oyster peptide; RAW264.7 cells; immunosuppression

Cite this article

LI Jinhong , ZHENG Huizhen , CHEN Hui , LIU Shulai , GU Saiqi , WANG Rui , XIANG Xingwei . The immunomodulatory effects of oyster peptide on mouse RAW264.7 cells[J]. Food and Fermentation Industries, 2023 , 49(22) : 49 -56 . DOI: 10.13995/j.cnki.11-1802/ts.033101

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