Escherichia coli O157∶H7 is a common foodborne pathogen, which can spread through fecal-oral transmission. Humans can be infected by ingesting foods and water contaminated with E. coli O157∶H7, which can cause various symptoms. Therefore, in order to detect E. coli O157∶H7 in the early stage, AuNPs and AuNFs were prepared as labeled probes by chemical reduction method and mediated growth method, and two immunochromatographic test strips for rapid detection of E. coli O157∶H7 were established. The sensitivity, specificity, and accuracy of two test strips were tested. The results showed that E. coli O157: H7 in food could be detected within 15 min by both methods. The lowest detection limits of AuNP and AuNFs were 3.2×105 and 3.2×104 CFU/mL, respectively. There was no cross reaction with common foodborne pathogens such as Listeria monocytogenes, Salmonella typhimurium, and Enterobacter sakazakii. AuNPs and AuNFs were detected in the samples of artificially contaminated jelly, milk, and beef after 5, 6 and 5 h, respectively, which were not affected by the complex mechanism of food. The results showed that both strips had high sensitivity, specificity, and simple operation, which were suitable for rapid detection of E. coli O157∶H7 on-site. This research provides a new idea for the detection of other bacteria and viruses.
WANG Yinglin
,
WU Yafang
,
LIU Cheng
,
HUANG Zhiqiang
,
LIU Kun
,
LIU Qing
. Establishment of gold nanoparticles and gold nanoflowers labeled immunochromatographic assay and comparison of rapid detection of Escherichia coli O157∶H7[J]. Food and Fermentation Industries, 2023
, 49(22)
: 287
-294
.
DOI: 10.13995/j.cnki.11-1802/ts.034746
[1] LUPINDU A M. Epidemiology of shiga toxin-producing Escherichia coli O157∶H7 in Africa in review[J]. Southern African Journal of Infectious Diseases, 2018, 33(1):24-30.
[2] CHA P I, GURLAND B, FORRESTER J D. First reported case of intussusception caused by Escherichia coli O157∶H7 in an adult: Literature review and case report[J]. Surgical Infections, 2019, 20(1):95-99.
[3] SHAN S, LIU D F, GUO Q, et al. Sensitive detection of Escherichia coli O157∶H7 based on cascade signal amplification in ELISA[J]. Journal of Dairy Science, 2016, 99(9):7025-7032.
[4] DINH A, ANATHASAYANAN A, RUBIN L M. Safe and effective use of eculizumab in the treatment of severe Shiga toxinEscherichia coli-associated hemolytic uremic syndrome[J]. American Journal of Health-System Pharmacy, 2015, 72(2):117-120.
[5] MOSSORO C, GLAZIOU P, YASSIBANDA S, et al. Chronic diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome associated with HEp-2 adherent Escherichia coli in adults infected with human immunodeficiency virus in Bangui, Central African Republic[J]. Journal of Clinical Microbiology, 2002, 40(8):3086-3088.
[6] GWAVAVA C, CHIHOTA V N, GANGAIDZO I T, et al. Dysentery in patients infected with human immunodeficiency virus in Zimbabwe: An emerging role forSchistosoma mansoni and Escherichia coli O157?[J]. Annals of Tropical Medicine & Parasitology, 2001, 95(5):509-513.
[7] ABONG′O B O, MOMBA M N B, MALAKATE V K, et al. Prevalence of Escherichia coli O157∶H7 among diarrhoeic HIV/AIDS patients in the eastern cape province-south Africa[J]. Pakistan Journal of Biological Sciences, 2008, 11(8):1066-1075.
[8] GRIFFIN P M, TAUXE R V. The epidemiology of infections caused by Escherichia coli O157∶H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome[J]. Epidemiologic Reviews, 1991, 13(1):60-98.
[9] MOEZI P, KARGAR M, DOOSTI A, et al. Multiplex touchdown PCR assay to enhance specificity and sensitivity for concurrent detection of four foodborne pathogens in raw milk[J]. Journal of Applied Microbiology, 2019, 127(1):262-273.
[10] CHEN H, LI Y K, ZHANG T T, et al. A novel real-time loop-mediated isothermal amplification combined with immunomagnetic beads separation and ethidium bromide monoazide treatment for rapid and ultrasensitive detection of viable Escherichia coli O157∶H7 in milk[J]. Food Analytical Methods, 2021, 14(5):944-956.
[11] BARAKETI A, D′AURIA S, SHANKAR S, et al. Novel spider web trap approach based on chitosan/cellulose nanocrystals/glycerol membrane for the detection of Escherichia coli O157∶H7 on food surfaces[J]. International Journal of Biological Macromolecules, 2020, 146:1009-1014.
[12] XING K Y, PENG J, LIU D F, et al. Novel immunochromatographic assay based on Eu (III)-doped polystyrene nanoparticle-linker-monoclonal antibody for sensitive detection of Escherichia coli O157∶H7[J]. Analytica Chimica Acta, 2018, 998:52-59.
[13] LIU C, FANG S Q, TIAN Y C, et al. Rapid detection of Escherichia coli O157∶H7 in milk, bread, and jelly by lac dye coloration-based bidirectional lateral flow immunoassay strip[J]. Journal of Food Safety, 2021, 41(1): e12862.
[14] LI Y Y, WANG Q, BERGMANN S M, et al. Preparation of monoclonal antibodies against KHV and establishment of an antigen sandwich ELISA for KHV detection[J]. Microbial Pathogenesis, 2019, 128:36-40.
[15] HUANG X Y, HUANG T, LI X J, et al. Flower-like gold nanoparticles-based immunochromatographic test strip for rapid simultaneous detection of fumonisin B1 and deoxynivalenol in Chinese traditional medicine[J]. Journal of Pharmaceutical and Biomedical Analysis, 2020, 177:112895.
[16] PENG T, SUI Z W, HUANG Z H, et al. Point-of-care test system for detection of immunoglobulin-G and-M against nucleocapsid protein and spike glycoprotein of SARS-CoV-2[J]. Sensors and Actuators B: Chemical, 2021, 331: 129415.
[17] JI Y W, REN M L, LI Y P, et al. Detection of aflatoxin B1 with immunochromatographic test strips: Enhanced signal sensitivity using gold nanoflowers[J]. Talanta, 2015, 142:206-212.
[18] ZHANG A M, GUO W W, KE H, et al. Sandwich-format ECL immunosensor based on Au star@BSA-Luminol nanocomposites for determination of human chorionic gonadotropin[J]. Biosensors and Bioelectronics, 2018, 101:219-226.
[19] ZHANG L, HUANG Y J, WANG J Y, et al. Hierarchical flowerlike gold nanoparticles labeled immunochromatography test strip for highly sensitive detection of Escherichia coli O157∶H7[J]. Langmuir, 2015, 31(19): 5537-5544.
[20] WU Y X, WU M J, LIU C, et al. Colloidal gold immunochromatographic test strips for broad-spectrum detection of Salmonella[J]. Food Control, 2021, 126:108052.
[21] WU M J, WU Y X, LIU C, et al. Development and comparison of immunochromatographic strips with four nanomaterial labels: Colloidal gold, new colloidal gold, multi-branched gold nanoflowers and Luminol-reduced Au nanoparticles for visual detection of Vibrio parahaemolyticus in seafood[J]. Aquaculture, 2021, 539:736563.
[22] WANG J H, HE K X, WU Z J, et al. Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo[J]. Foods, 2022, 11(5):756.
[23] PULIGUNDLA P, LIM S. Biocontrol approaches against Escherichia coli O157∶H7 in foods[J]. Foods, 2022, 11(5):756.
