Growth characteristics and stress tolerance of plasmid-mediated ampicillin-resistant Salmonella Enteritidis

  • HONG Yi ,
  • XIE Yani ,
  • WU Yufan ,
  • QIN Xiaojie ,
  • DONG Qingli ,
  • WANG Xiang
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  • 1(School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
    2(School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China)

Received date: 2023-02-13

  Revised date: 2023-03-18

  Online published: 2024-01-02

Abstract

Salmonella Enteritidis is an important foodborne pathogen, its antibiotic resistance is a global concern. The widespread dissemination of antibiotic-resistant plasmids has increased the antibiotic resistance of S. Enteritidis. The acquisition of antibiotic-resistant plasmids may affect bacterial growth and stress tolerance, which poses new challenges for controlling antibiotic-resistant foodborne pathogens. This study aimed to examine the impact of plasmid-mediated ampicillin resistance on the growth and stress tolerance of S. Enteritidis. The pKD46 plasmid containing the ampicillin resistance gene was introduced into antibiotic-sensitive S. Enteritidis strains. The growth characteristics, acid and heat resistance of the antibiotic-resistant S. Enteritidis were then evaluated. The results indicated that the resistance of transformed S. Enteritidis to other antibiotics remained unchanged; the maximum specific growth rate (μmax) showed nonsignificant difference and its lag phase duration (λ) was prolonged. The heat resistance of the transformed S. Enteritidis reduced significantly at 57.5 ℃ and 60 ℃, but its acid resistance to pH 3.0 remained stable. In conclusion, this study found that plasmid-mediated ampicillin resistance can reduce the growth and heat resistance of S. Enteritidis, which can provide a reference for the control and risk assessment of antibiotic-resistant foodborne pathogens.

Cite this article

HONG Yi , XIE Yani , WU Yufan , QIN Xiaojie , DONG Qingli , WANG Xiang . Growth characteristics and stress tolerance of plasmid-mediated ampicillin-resistant Salmonella Enteritidis[J]. Food and Fermentation Industries, 2023 , 49(23) : 70 -75 . DOI: 10.13995/j.cnki.11-1802/ts.035114

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